Publication Date:
2021-07-08
Description:
Regarding low fertilization rate of Barbus sharpeyi, one of the main Barbus species in south of Iran, saline activator solutions (SAS 1 and 2) were used for increasing of sperm activation and its sperm quality indices were assessed by using Image J software as a fish sperm adapted tools. SAS1 (45mM NaCl, 5mM KCl, 30mM Tris, 188±2.3 mOsmol/kg) and SAS2 (50mM NaCl, 30mM KCl, 30mM Tris, 189±1.7 mOsmol.kg) were added to a mixture of 12 male semen comparing with distilled and tap water and their effects on VCL, VSL, ALH, Linearity, %motility, spermatozoa path, % A, B and C spermatozoa type, fertilization, hatching and deformity rate were analyzed in triplicate. Results showed that SAS2 had the highest VCL (21.37±0.36 µm/s), VSL (11.6±0.3 µm/s) and motility percentage (85.75±1.56 %) among treatments. The highest A type spermatozoa percentage was recorded for SAS1 (47.16±3.15 %) and SAS2 (43.89±4.54%). The lowest C type spermatozoa percentage was recorded using SAS2 (14.24±1.56 %). Also, the highest fertilization rate was achieved from SAS2 (79.5±0.37%) and SAS1 (77.74±4%) respectively. Furthermore the lowest larval deformity rate (13.77±1.03%) was shown due to the use of SAS2. Final conclusion confirmed that SAS1 and SAS2 have positive and significant effects (P 〈 0.05) on extending the time length of sperms movement, VCL and VSL of B. sharpeyi which consequently resulted in better efficiency of propagation of this species in south of Iran.
Keywords:
Biology
;
Fisheries
;
Sperm
;
Saline activator solution
;
Fertilization
;
Barbus sharpeyi
;
Iran
Repository Name:
AquaDocs
Type:
article
,
TRUE
Format:
application/pdf
Format:
application/pdf
Format:
357-377
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