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  • Iranian Fisheries Science Research Institute  (18)
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Keywords
Publisher
  • Iranian Fisheries Science Research Institute  (18)
  • Tehran, Iran  (18)
Years
  • 11
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    Effect of Echinacea purpurea and Astragalus glycyphyllos on immune responses of rainbow trout (O. mykiss) and resistance to sterptococcosis (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25451 | 18721 | 2018-09-23 17:53:51 | 25451 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Nowadays, medical plants extract is used in aquaculture industry to enhance growth, immune system and fish resistance to different pathogens. In this study, some non-specific immune responses, hematology and biochemical parameters in rainbow trout juveniles (16 gr mean weight) and their resistance to Streptococcus iniae were evaluated following oral administration of Echinacea purpurea and Astragalus sp. extract. Three concentrations (0.5, 1 and 1.5 gr/kg of feed) of Echinacea purpurea and three concentrations (2, 3 and 5 gr/kg of feed) of Astragalus sp extract and control group were used for 60 days. Parameters evaluated included: levels of C4, C3 (complement components), free oxygen radicals (respiratory burst), Lysozyme, numbers of lymphocytes, monocytes and neutrophils. In the end, the relative survival rate (RSR) of fish was evaluated against S. iniae. The results showed that the levels of C3, lysozyme, oxygen free radicals, the number of neutrophils and lymphocytes in the experiment groups (the highest dose) compared to control group were increased significantly (p 〈0/05). While, the values of C4 and number of monocytes compared to the control group were not significantly different (p〉 0.05). The relative survival rates of fish following challenge with Streptococcus iniae, were 91/11, 93/33 and 44/44 percent in experiments (Echinacea purpurea and Astragalus sp.) and control groups, respectively. In conclusion, it may be state that Echinacea purpurea and Astragalus sp. extracts enhance the non-specific immune system and fish resistance against Streptococcusis, and it seems it can be used as immunostimulant in feed.
    Keywords: Aquaculture ; Iran ; Echinacea purpurea ; Astragalus sp ; Rainbow trout ; Streptococcusis ; Immune system ; Astragalus glycyphyllos ; O.mykiss ; Aquaculture ; Growth ; Juvenile ; Lysozyme ; Survival rate
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 106
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  • 12
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    Production of bacterial probiotic from trout (Oncorhynchus mykiss) for improvement of immune system and challenge to streptococcosis (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25649 | 18721 | 2018-10-08 08:09:48 | 25649 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study was conducted for the first time and the effects of probiotic bacteria isolated from the gut of Rainbow trout (Oncorhynchus mykiss). In the first step, after the purification of the isolated bacteria, probiotic properties in vitro was assessed with injection to healthy fish and challenge with three species of pathogenic bacteria in culture media. The second phase of the bacteria isolated in 5 treatments (log 7, 8 and 9 of the lactic bacteria (LAB), Vibrio sp and Pseudomonas sp) and a control treatment on growth and survival, hematological parameters (RBC and WBC cells), immunology and physiology parameters tested during the 60 days of the above parameters after 30 and 60 days and finally at the end, the final assessment was Streptococcosis resistance in fish. The results showed that the isolated bacteria were able to enhance the growth parameters (weight, feed conversion, feed fat, protein efficiency ratio and specific growth rate) and survival. The results showed that log 8 LAB was significant difference with other treatments and control. When using log 8 LAB and Vibrio, the MCV, MCH and MCHC were decreased with no significant difference. The log 8 has the greatest effect on the amount of liver enzyme (AST), IgM and complement component C3, and had significant difference with other treatments. The challenge examination to Streptococcus iniae showed the highest survival in treatments with log 8 (96.66%), and Vibrio (93.33%) and then subjected to other treatments and control are also the least survival (25.38%). The conclusion of that study is the first probiotic properties of bacteria isolated from trout to changes in quality indicators are in particular the LAB bacteria and the second log 8 of LAB had significant positive of changes development and safety, and the fish are resistanted against Streptococcosis.
    Keywords: Aquaculture ; Health ; Iran ; LAB ; Rainbow trout ; Growth ; Immune system ; Streptococcosis ; Bacterial ; Oncorhynchus mykis ; Pathogenic ; Survival ; Hematological parameters
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 70
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  • 13
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    Survey on achieving recombinant DNA vaccine (live delivery) against streptococcosis for Rainbow trout (Oncorhynchus mykiss) immunization (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25730 | 18721 | 2018-10-10 10:17:07 | 25730 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The main purpose of this study was survey on achieving recombinant DNA vaccine (live delivery) against streptococcusis for Rainbow trout (Onchorhynchus mykiss) Immunization. Initially, a total of 515 samples were collected from the head kidney of diseased fish (weigh 50_ 200g) in 72 farms of 8 provinces. Approximately, 40% (206 samples) of specimens were infected to Streptococcus species. Then were isolated 172 DNA samples and consequently, five pathogenic species have been identified, including S. iniae, S. faecium, S. agalactiae, S. dysgalatiae, and S. uberis. The enzyme phosphoglucomutase (PGM) has recently been discovered to play an important role in polysaccharide capsule production and virulence in S. iniae. Therefore, was initially isolated S. iniae and cloning phosphoglucomutase gene. Then, the PGM gene was amplified successfully and cloned in pTZ57R cloning vector. The recombinant plasmid was sub cloned into pETD uet-l expression vector by restriction enzymes and confirmed by PCR. Meanwhile, for amplifying simA and cpsD genes were used universal primers pNZ8148 and special for simA and cpsD genes.The recombinant bacteria Lactococcus lactis (NZ9000) was used for transformation the plasmid into Lactococcus lactis. Vaccination was performed by oral, bath and injection (peritoneal) methods. The efficiency of g2 was better than g1in these three methods and all of groups. The determination of IgM level, or detection of anti S. iniae antibody was carried out by using ELISA. The results revealed that there was a significant (p〈0.05) difference between the level of IgM in all three methods and experiment groups compare to control group. The results of challenge of vaccinated fish with S. iniae showed that fish RPS in all of groups except group 3 (42.63 percent) were more than 50 percent while, in control group was 21.43 percent. The highest fish RPS was belong to group 11(61.25 percent) and statistical analyses revealed that significant (p〈0.05) difference between fish vaccinated RPS, compare to control group.
    Keywords: Biology ; Health ; Iran ; Survey ; DNA ; Live delivery ; Rainbow trout ; Onchorhynchus mykiss ; Immunization
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 82
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  • 14
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    Rearing of common carp (Cyprinus carpio) in fiber glass tanks with pellet feeding (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25317 | 18721 | 2018-09-11 09:06:52 | 25317 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The intensive culture of common carp (Cyprinus carpio) evaluated in fibreglass tanks (2*2m) with feeding on pellet during 8 weeks. The fish (n=201)of common carp selected from three treatments along with three replicates as the average of fish weight was less than 100(g )which divided into 〈50(g), 50-70(g) and 70-100(g).At the first experiment , there is no statistically significant difference between fish weight in relative to replicate treatment (P〉0.05).The common pellet food was chosen for feeding from market as fish were fed by the mean of 4% of their body weight a day. The result indicated that the mean of particular growth ratio at first treatment (0.54) has higher than second treatment (0.34) but in comparison with third treatment (0.46), there was no statistically significant difference (P〈0.05).Also, the conversion ratio at first treatment (5.28) and third treatment (6.09) was lower than second treatment (35.66) and third treatment (20.91) was lower than second treatment (24.24), (P〈0.05). This study showed that monoculture in small scale is feasible by utilizing artificial diet, if the water quality is exposed to change. In this survey, the conversion ratio of the fish was relatively high whereas the growth rate was low.
    Keywords: Aquaculture ; Iran ; Common carp ; Mono culture ; Pellet ; Fiber glass tank ; Cyprinus carpio ; Feeding ; Weight ; Growth Rate ; Artificial diet
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 21
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  • 15
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    Study of indicator bacteria and ovary of nematode at the surface water, underwater and sewage in the basin of Mangol dam (Mazandaran province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25529 | 18721 | 2018-10-05 15:51:43 | 25529 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Indicator of bacteria such total coliforms and fecal coliforms are one of the most important environmental factors that effect on water quality. Haraz river is one of three rivers in north of country and it originate from central Alborz. Overall, discharge of unborn sewage, agriculture pesticides, seasonal raining was caused decreasing of quality of the river. Therefore, quality and quantity of the grandwater, surface water and sewage in different zone of this river such Mangol dam is necessary. In this study, total coliforms and fecal coliforms and nematode ovary in 13 stations of Haraz including Sorkhrood, Klodeh, Amol Esar Town, Helomsar, Mangol dam, Karesang, Jalav bridge, Norroud, Tehran 115, Larijan, Abeask, Lasem, Lar (pleur) were surveyed. ECC Chrome agar was used for counting and isolation of coliforms groups and nematode ovary counting was done as formal – ether sedimentation method. The results showed that maximum and minimum of total coliforms in surface water was observed in Sorkhrood (9.4 CFU/100ml) and Lasem (4.2CFU/100ml) and also maximum and minimum of fecal coliforms in Sorkhrood (3/2 CFU / ml) and Lasem (1/1CFU/100ml) respectively. Maximum and minimum of these bacteria in ground water were Abeask (4 CFU /100ml) and Helomsar (9/1 CFU/100ml) (for total coliforms) and Abeask (7/1 CFU/100ml) and Helomsar, Klodeh and Larijan (2/1 CFU/100ml) (for fecal coliforms) respectively. Maximum and minimum of total and fecal coliforms in sewage were Norroud (1/3 CFU/100ml) and Amole Esar Town (2/2 CFU/100ml) (for total coliforms) and Norroud (6/1 CFU/100ml) and Amole Esar Town and Tehran 115 (2/1 CFU/100ml) (for fecal coliforms) respectively. Ovary of Nematode in sewage was 30 and 124 in Norroud and Amole Esar Town respectively. The conclusion of this study was showed that quality of Haraz is low level and not proper for drinking and swimming.
    Keywords: Health ; Iran ; Mazandaran province ; Haraz River ; Mangal Dam ; Surface water ; Groundwater ; Sewage ; Coliforms ; Nematodes ; Bacteria ; Agriculture ; Sedimentation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 48
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  • 16
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    A survey on some risk factors and evaluation of their impacts on streptococcosis in rainbow trout farms in some provinces in Iran (Mazandaran, Fars) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25613 | 18721 | 2018-10-14 02:35:34 | 25613 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: One of the most important bacterial fish diseases which has caused some outbreaks in rainbow trout farms in Iran is streptococcusis. The farmers were suffering from huge economic losses due to the disease outbreaks in different rainbow trout farms in Iran. The aim of our study was to determine rate of streptococcusis incidence in different stage of growth in farmed rainbow trout in Mazandaran and Fars province. Fish and water samples were randomly collected and measured randomly in selected farms, monthly throughout a year. After clinical observations, Isolation and recognition of strep strains were made using biochemical and molecular tests. Some Environmental factors include Nitrate, Nitrite, Temperature, pH, Ammonia and DO measure during sampling periods. According to our results incidence of disease in juvenile is more than growers. Some samples showed clinical signs of streptococcusis without strep. contamination. Main isolated strain were S. iniae and S. garviea and S. uberis recognized for first time in east of Mazandaran province (Haraz River). Incidence of streptococcusis in rainbow trout affected by fluctuation of Nitrite, temperature and DO. Management of these factors can decrease rate of disease outbreaks.
    Keywords: Health ; Iran ; Mazandaran province ; Fars province ; Streptococcusis ; Incidence ; Rainbow trout ; Environmental factors ; Survey ; Streptococcosis ; Bacterial ; Diseases ; Growth ; Samples ; S.iniae ; S.garviea ; S.uberis
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 156
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  • 17
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    A survey on some risk factors and evaluation of their impacts on streptococcosis in rainbow trout farms in some provinces in IRAN. (Mazandaran, Fars) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: One of the most important bacterial fish diseases which has caused some outbreaks in rainbow trout farms in Iran is streptococcusis .The farmers were suffering from huge economic losses due to the disease outbreaks in different rainbow trout farms in Iran. The aim of our study was to determine rate of streptococcusis incidence in different stage of growth in farmed rainbow trout in Mazandaran and Fars province. Fish and water samples were randomly collected and measured randomly in selected farms, monthly throughout a year. After clinical observations, Isolation and recognition of strep strains were made using biochemical and molecular tests. Some Environmental factors include Nitrate, Nitrite, Temperature, pH, Ammonia and DO measure during sampling periods. According to our results incidence of disease in juvenile is more than growers. Some samples showed clinical singe of streptococcusis without strep. Contamination .Main isolated strain were S.iniae and S.garviea and S.uberis recognized for first time in east of Mazandaran province (Haraz River). Incidence of streptococcusis in rainbow trout affected by fluctuation of Nitrite, temperature and DO. Management of these factors can decrease rate of disease outbreaks.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Clinical ; Biochemical ; Environmental ; Streptococcusis ; Incidence ; Rainbow trout ; Environmental factors ; Survey ; Streptococcosis ; Bacterial ; Diseases ; Growth ; Samples ; S.iniae ; S.garviea ; S.uberis
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 156pp.
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  • 18
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    Effect of Echinacea purpurea and Astragalus glycyphyllos on immune responses of Rainbow trout (O.mykiss) and resistance to sterptococcosis (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Nowadays, medical plants extract is used in aquaculture industry to enhance growth, immune system and fish resistance to different pathogens. In this study, some non-specific immune responses, hematology and biochemical parameters in rainbow trout juveniles (16 gr mean weight) and their resistance to Streptococcus iniae were evaluated following oral administration of Echinacea purpurea and Astragalus sp. extract. Three concentrations (0.5, 1 and 1.5 gr/kg of feed) of Echinacea purpurea and three concentrations (2, 3 and 5 gr/kg of feed) of Astragalus sp extract and control group were used for 60 days. Parameters evaluated included: levels of C4, C3 (complement components), free oxygen radicals (respiratory burst), Lysozyme, numbers of lymphocytes, monocytes and neutrophils. In the end, the relative survival rate (RSR) of fish was evaluated against S. iniae. The results showed that the levels of C3, lysozyme, oxygen free radicals, the number of neutrophils and lymphocytes in the experiment groups (the highest dose) compared to control group were increased significantly (p 〈0/05). While, the values of C4 and number of monocytes compared to the control group were not significantly different (p〉 0.05). The relative survival rates of fish following challenge with Streptococcus iniae, were 91/11, 93/33 and 44/44 percent in experiments (Echinacea purpurea and Astragalus sp.) and control groups, respectively. In conclusion, it may be state that Echinacea purpurea and Astragalus sp. extracts enhance the non-specific immune system and fish resistance against Streptococcusis, and it seems it can be used as immunostimulant in feed.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Hematology ; Biochemical ; Echinacea purpurea ; Astragalus sp ; Rainbow trout ; Streptococcusis ; Immune system ; Astragalus glycyphyllos ; O.mykiss ; Aquaculture ; Growth ; Juvenile ; Lysozyme ; Survival rate
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 106pp.
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