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  • Bacillus acidocaldarius  (2)
  • Invertebrate phototransduction  (2)
  • Key words Wheat  (2)
  • Pollen selection  (2)
  • Springer  (8)
  • Geological Society of America (GSA)
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Verlag/Herausgeber
  • Springer  (8)
  • Geological Society of America (GSA)
Erscheinungszeitraum
  • 1
    Digitale Medien
    Digitale Medien
    The effect of temperature and pH on the growth efficiency of the thermoacidophilic bacterium Bacillus acidocaldarius in continuous culture (1983)
    Springer
    Archives of microbiology 135 (1983), S. 276-283 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Molar growth yields ; Thermoacidophile ; Respiratory chain energy conservation ; Membrane permeability ; Temperature and pH ; Bacillus acidocaldarius
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The molar yield coefficients (Y glucose, Y O 2) of glucose-limited continuous cultures of the thermoacidophile Bacillus acidocaldarius have been measured as a function of dilution rate as well as over a range of temperature and pH (51°C to 64°C, pH 2.8–5.5) at a fixed dilution rate of approximately 0.1 h-1. The highest growth yields were observed at 51°C and pH〉4.3 (Y glucose 54.8 g cells · mol glucose-1, Y O 2 15.0 g cells · mol O 2 -1 ), but were very much lower than those of mesophilic neutrophiles of similar respiratory chain composition to B. acidocaldarius. Even lower growth yields were observed when the temperature was raised or when the pH was lowered, lowest yields occurring at 64°C and pH 2.8 (Y glucose 23.4 g cells · mol glucose-1, Y O 2 5.9 g cells · mol O 2 -1 ). These decreases in growth yield could be correlated with increases in the permeability of the cytoplasmic membrane to protons, i.e. cells needed to catalyse enhanced rates of substrate oxidation in order to avoid a potentially lethal acidification of the cytoplasm. This strategy appears to be successful in that the specific death rates in situ were very low for all cultures except those growing under the most extreme conditions (64°C, pH 2.8).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00413481
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    The use of response surface analysis to study the growth of Bacillus acidocaldarius throughout the growth range of temperature and pH (1983)
    Springer
    Archives of microbiology 135 (1983), S. 272-275 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Bacillus acidocaldarius ; Response surface analysis ; Temperature and pH
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A minimal-salts medium has been optimised to support the growth of the acidophilic thermophilic bacterium Bacillus acidocaldarius. This medium was used during a study of the effect of temperature and pH on the growth rate and growth yield of this organism in batch cultures; a statistical method was used to design the experimental points, and the data were subjected to a response surface analysis which allowed the growth rate and growth yield to be predicted over the entire temperature and pH range from a minimum number of experimental points. The results indicate different responses for growth rate (optimum, 60°C, pH 4.1) and growth yield (optimum tending towards low temperature and neutral pH).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00413480
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  • 3
    Digitale Medien
    Digitale Medien
    Effects of pollen selection on progeny vigor in a Cucurbita pepo × C. texana hybrid (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 885-890 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words  Microgametophyte selection ; Pollen selection ; Pollen competition ; Cucurbita texana ; Cucurbita pepo
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract   We examined the effects of pollen selection for rapid pollen-tube growth on progeny vigor. First, we crossed a wild gourd (Cucurbita texana) to a cultivated zucchini (Cucurbita pepo cv `Black Beauty') to produce an F1 and then an F2 generation. Half of the F1 seeds were produced by depositing small loads of C. texana pollen onto the stigmas of C. pepo. These small pollen loads were insufficient to produce a full complement of seeds and, consequently, both the fast- and the slow-growing pollen tubes were permitted to achieve fertilization. An F2 generation was then produced by depositing small loads of F1 pollen onto stigmas of F1 plants. The F2 seeds resulting from two generations of small pollen loads are termed 'the non-selected line' because there was little or no selection for pollen-tube growth rate on these plants. The other half of the F1 and F2 seeds were produced by depositing large pollen loads (〉10000 pollen grains) onto stigmas and then allowing only the first 1% or so of the pollen tubes that entered the ovary to fertilize the ovules. We did this by excising the styles at the ovary at 12–15 h after pollination. The resulting F2 seeds are termed `the selected line' because they were produced by two generations of selection for only the fastest growing pollen tubes. Small pollen loads from the F2 plants, both the selected and the non-selected lines, were then deposited onto stigmas of different C. pepo flowers, and the vigor of the resulting seeds was compared under greenhouse and field conditions. The results showed that the seeds fertilized by pollen from the selected line had greater vegetative vigor as seedlings and greater flower and fruit production as mature plants than the seeds fertilized by pollen from the non-selected line. This study demonstrates that selection for fast pollen-tube growth (selection on the microgametophyte) leads to a correlated increase in sporophyte (progeny) vigor.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050207
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  • 4
    Digitale Medien
    Digitale Medien
    Effects of pollen selection on progeny vigor in a Cucurbita pepo x C. texana hybrid (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 885-890 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Microgametophyte selection ; Pollen selection ; Pollen competition ; Cucurbita texana ; Cucurbita pepo
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We examined the effects of pollen selection for rapid pollen-tube growth on progeny vigor. First, we crossed a wild gourd (Cucurbita texana) to a cultivated zucchini (Cucurbita pepo cv ‘Black Beauty’) to produce an F1 and then an F2 generation. Half of the F1 seeds were produced by depositing small loads of C. texana pollen onto the stigmas of C. pepo. These small pollen loads were insufficient to produce a full complement of seeds and, consequently, both the fast- and the slow-growing pollen tubes were permitted to achieve fertilization. An F2 generation was then produced by depositing small loads of F1 pollen onto stigmas of F1 plants. The F2 seeds resulting from two generations of small pollen loads are termed the non-selected line because there was little or no selection for pollen-tube growth rate on these plants. The other half of the F1 and F2 seeds were produced by depositing large pollen loads (〉10 000 pollen grains) onto stigmas and then allowing only the first 1% or so of the pollen tubes that entered the ovary to fertilize the ovules. We did this by excising the styles at the ovary at 12–15 h after pollination. The resulting F2 seeds are termed ‘the selected line’ because they were produced by two generations of selection for only the fastest growing pollen tubes. Small pollen loads from the F2plants, both the selected and the non-selected lines, were then deposited onto stigmas of different C. pepo flowers, and the vigor of the resulting seeds was compared under greenhouse and field conditions. The results showed that the seeds fertilized by pollen from the selected line had greater vegetative vigor as seedlings and greater flower and fruit production as mature plants than the seeds fertilized by pollen from the non-selected line. This study demonstrates that selection for fast pollen-tube growth (selection on the microgametophyte) leads to a correlated increase in sporophyte (progeny) vigor.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221902
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  • 5
    Digitale Medien
    Digitale Medien
    Isolation and characterisation of microsatellites from hexaploid bread wheat (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 557-563 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Wheat ; PCR ; Microsatellite ; Simple sequence repeats
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The development of large panels of simple-to-analyse genetic markers for tagging agronomically important genes and diversity studies in hexaploid bread wheat is an important goal in applied cereal genetic research. We have isolated and sequenced over 200 clones containing microsatellites from the wheat genome and have tested 153 primer pairs for genetic polymorphism using a panel of ten wheat varieties, including the parents of our main mapping cross. A subset comprising 49 primer pairs detects 76 loci, of which 74 can be unequivocably allocated to one of the wheat chromosomes. A relatively low frequency of the loci detected are from the D genome, and these loci show less polymorphism than those from the A and B genomes. Generally, the microsatellites show high levels of genetic polymorphism and an average of 3.5 alleles per locus with an average polymorphism information content (PIC), value of 0.51. The observed levels of polymorphism are positively correlated with the length of the microsatellite repeats. A high proportion, approximately two-thirds, of primer pairs designed to detect simple sequence repeat (SSR) variation in wheat do not generate the expected amplification products and, more significantly, often generate unresolvable PCR products. In general, our results agree closely with those obtained from other recent studies using microsatellites in plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050451
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  • 6
    Digitale Medien
    Digitale Medien
    Fifty new microsatellite loci for the wheat genetic map (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 946-949 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Wheat ; Microsatellite markers ; Genetic map
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Hexaploid bread wheat (Triticum aestivum) has low levels of RFLP. Simple sequence repeats, however, show high levels of polymorphism and are therefore especially useful in intervarietal breeding applications. We present 53 newly mapped microsatellite loci for the wheat genetic map, 41 primary loci and 12 additional loci from these same primer pairs. Markers have been accredited with a quality score on a scale of 1–5 which describes the complexity of the amplification product profile from each primer pair.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050975
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  • 7
    Digitale Medien
    Digitale Medien
    Dissociation of photoreceptors from whole heads of the fruit fly, Drosophila melanogaster (1995)
    Springer
    Cell & tissue research 280 (1995), S. 473-477 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Photoreceptors ; Ommatidia ; Tissue dissociation ; Enzymatic digestion ; Invertebrate phototransduction ; Electrophysiology ; Drosophila melanogaster (Insecta)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Photoreceptor cells that were mostly free of extracellular material and suitable for most electrophysiological study procedures were dissociated from whole heads of the fruit fly, Drosophila melanogaster, by a simple “smash” technique employing gentle chopping by a razor blade through Parafilm sheets. A variety of commonly available proteolytic and glycolytic digestion enzymes were tested as additions to the basic dissociation procedure described. With the aid of Nomarski interference contrast optics, periodic acid-Schiff staining, and fluorescent labeling and microscopy methods, it was determined that proteolytic enzymatic digestion does little to enhance the dissociation procedure, and instead, often damages the cells that one is attempting to recover. Unexpectedly, certain glycolytic enzymes, when added to the basic procedure, appear to enhance the recovery of intact viable Drosophila photoreceptors that are stripped of most extracellular material. Based on these results, a hypothesis concerning the biochemical nature of the extracellular matrix of the Drosophila retina is proposed. Drosophila photoreceptors are an interesting model system for the study of invertebrate phototransduction and photoreceptor cell biology because of their many well-characterized mutant strains. The technique described here should produce clean viable photoreceptors or ommatidia that respond to light, and that are suitable for patch clamping or cell culture.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00307821
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  • 8
    Digitale Medien
    Digitale Medien
    Dissociation of photoreceptors from whole heads of the fruit fly, Drosophila melanogaster (1995)
    Springer
    Cell & tissue research 280 (1995), S. 473-477 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Key words: Photoreceptors ; Ommatidia ; Tissue dissociation ; Enzymatic digestion ; Invertebrate phototransduction ; Electrophysiology ; Drosophila melanogaster (Insecta)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. Photoreceptor cells that were mostly free of extracellular material and suitable for most electrophysiological study procedures were dissociated from whole heads of the fruit fly, Drosophila melanogaster, by a simple ”smash” technique employing gentle chopping by a razor blade through Parafilm sheets. A variety of commonly available proteolytic and glycolytic digestion enzymes were tested as additions to the basic dissociation procedure described. With the aid of Nomarski interference contrast optics, periodic acid-Schiff staining, and fluorescent labeling and microscopy methods, it was determined that proteolytic enzymatic digestion does little to enhance the dissociation procedure, and instead, often damages the cells that one is attempting to recover. Unexpectedly, certain glycolytic enzymes, when added to the basic procedure, appear to enhance the recovery of intact viable Drosophila photoreceptors that are stripped of most extracellular material. Based on these results, a hypothesis concerning the biochemical nature of the extracellular matrix of the Drosophila retina is proposed. Drosophila photoreceptors are an interesting model system for the study of invertebrate phototransduction and photoreceptor cell biology because of their many well-characterized mutant strains. The technique described here should produce clean viable photoreceptors or ommatidia that respond to light, and that are suitable for patch clamping or cell culture.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00307821
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