Abstract
A minimal-salts medium has been optimised to support the growth of the acidophilic thermophilic bacterium Bacillus acidocaldarius. This medium was used during a study of the effect of temperature and pH on the growth rate and growth yield of this organism in batch cultures; a statistical method was used to design the experimental points, and the data were subjected to a response surface analysis which allowed the growth rate and growth yield to be predicted over the entire temperature and pH range from a minimum number of experimental points. The results indicate different responses for growth rate (optimum, 60°C, pH 4.1) and growth yield (optimum tending towards low temperature and neutral pH).
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Abbreviations
- NTA:
-
nitrilotriacetic acid
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Farrand, S.G., Linton, J.D., Stephenson, R.J. et al. The use of response surface analysis to study the growth of Bacillus acidocaldarius throughout the growth range of temperature and pH. Arch. Microbiol. 135, 272–275 (1983). https://doi.org/10.1007/BF00413480
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DOI: https://doi.org/10.1007/BF00413480