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  • ASTROPHYSICS  (805)
  • Animals  (615)
  • Chemical Engineering  (564)
  • SPACE RADIATION  (527)
  • Life and Medical Sciences  (404)
  • 1980-1984  (2,191)
  • 1970-1974  (716)
  • 1890-1899
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  • 1
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    Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine (1981)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1981-12-04
    Description: A DNA sequence coding for the immunogenic capsid protein VP3 of foot-and-mouth disease virus A12, prepared from the virion RNA, was ligated to a plasmid designed to express a chimeric protein from the Escherichia coli tryptophan promoter-operator system. When Escherichia coli transformed with this plasmid was grown in tryptophan-depleted media, approximately 17 percent of the total cellular protein was found to be an insoluble and stable chimeric protein. The purified chimeric protein competed equally on a molar basis with VP3 for specific antibodies to foot-and-mouth disease virus. When inoculated into six cattle and two swine, this protein elicited high levels of neutralizing antibody and protection against challenge with foot-and-mouth disease virus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kleid, D G -- Yansura, D -- Small, B -- Dowbenko, D -- Moore, D M -- Grubman, M J -- McKercher, P D -- Morgan, D O -- Robertson, B H -- Bachrach, H L -- New York, N.Y. -- Science. 1981 Dec 4;214(4525):1125-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6272395" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Antibody Formation ; Base Sequence ; Cattle ; Cattle Diseases/*prevention & control ; *Cloning, Molecular ; DNA Restriction Enzymes ; DNA, Recombinant/metabolism ; Foot-and-Mouth Disease/*prevention & control ; Immunity, Cellular ; Protein Biosynthesis ; Swine ; Swine Diseases/*prevention & control ; Transcription, Genetic ; *Vaccines ; Viral Proteins/genetics/*therapeutic use
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
    Electronic Resource
    Electronic Resource
    Perturbation of the switch-on of transcriptase activity in intermediate subviral particles from reovirus (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 10-18 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch-on of transcriptase function. In such particles the endogenous transcriptase is present in a switched-off form, one step removed from the switched-on state. Switch-on of transcriptase function is an active process in this system and can be triggered by K+ ions. A variety of agents which affect gene expression in cells were tested for an effect on switch-on in ISVP. Marked effects on switch-on in ISVP were observed with a diverse group of test agents, including DMSO and other solvents, BUdR, TdR, caffeine, theophylline, and temperature. The correlation in response between ISVP and cells suggests that the ISVP system may be useful as a model for studying the biochemical mechanisms underlying the perturbative effects of such agents on gene expression in cells.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041120104
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Inhibition of the metabolism of ram spermatozoa by derivatives of α-chlorohydrin (1981)
    New York, NY : Wiley-Blackwell
    Gamete Research 4 (1981), S. 203-217 
    Details
    ISSN: 0148-7280
    Keywords: α-chlorohydrin ; antifertility agent ; ram ; sperm metabolism ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effects of the male antifertility agent, α-chlorohydrin, six of its derivatives, and glycidol were studied on the metabolism of washed ram spermatozoa in vitro with fructose as substrate. The α-chlorohydrin derivatives were the amino, the phosphorylated, and four glycol-bridge (ketal) compounds. All compounds except glycidol, in a concentration between 0.1 and 100 mM, reduced the aerobic glycolsis and/or oxidation of fructose. However, there was not a high correlation between the ability of these compounds to inhibit the metabolism of ram spermatozoa in vitro and their antifertility activity when administered to male rats. Other factors are clearly involved in their antifertility activity, eg, the concentration of the compounds in the epididymis and their conversion of either more or less spermicidal compounds in the body.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120040305
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  • 4
    Electronic Resource
    Electronic Resource
    Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)
    New York, NY : Wiley-Blackwell
    Gamete Research 10 (1984), S. 339-351 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120100402
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  • 5
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    Giant panda paternity (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-03-16
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉O'Brien, S J -- Goldman, D -- Knight, J -- Moore, H D -- Wildt, D E -- Bush, M -- Montali, R J -- Kleiman, D -- New York, N.Y. -- Science. 1984 Mar 16;223(4641):1127-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6701515" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Carnivora ; Male ; *Paternity ; Polymorphism, Genetic ; Proteins/analysis
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 6
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    Pioneer 10 observations of the solar wind interaction with Jupiter (1974)
    In:  Other Sources
    Details
    Publication Date: 2011-08-16
    Description: Detailed analysis of the Pioneer 10 plasma analyzer experiment flight data during the Jupiter flyby in late November and early December 1973 has been performed. The observations show that the interaction of Jupiter's magnetic field with the solar wind is similar in many ways to that at earth, but the scale size is over 100 times larger. Jupiter is found to have a detached standing bow shock wave of high Alfven Mach number. Like the earth, Jupiter has a prominent magnetopause that deflects the magnetosheath plasma and excludes its direct entry into the Jovian magnetosphere. Unlike that of the earth, the sunward hemisphere of Jupiter's outer magnetosphere is found to be highly inflated with thermal plasma and a high-beta region that is highly responsive to changes in solar wind dynamic pressure.
    Keywords: SPACE RADIATION
    Type: Journal of Geophysical Research; 79; Sept. 1
    Format: text
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  • 7
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    Arcsecond Positions for Milliarcsecond VLBI Nuclei of Extragalactic Radio Sources, Part 2: 207 Sources (1984)
    In:  CASI
    Details
    Publication Date: 2014-09-11
    Description: Very long base interferometry measurements of time delay and fringe frequency at 2.29 GHz on baselines of 10,000 km between Deep Space Network stations were used to determine the positions of the milliarcsecond nuclei in 207 extragalactic radio sources. Estimated accuracies generally range from approximately 0.1 to approximately 1.0, in both right ascension and declination, with all sources having uncertainties 4" in both coordinates. The observed sources are part of an all-sky VLBI catalog of milliarcsecond radio sources. Arcsecond positions are now determined for 752 of these sources. Arcsecond positions serve as a useful starting point in the construction of high-precision VLBI reference frames and are also important for unambiguous determination of optical counterparts to compact radio sources.
    Keywords: SPACE RADIATION
    Type: The Telecommun. and Data Acquisition Rept.; p 1-11
    Format: text
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    NASA TECHNICAL REPORTS
  • 8
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    Pioneer 10 observations of the solar wind interation with Jupiter (1974)
    In:  CASI
    Details
    Publication Date: 2019-06-27
    Description: Pioneer 10 Plasma Analyzer experiment flight data during the Jupiter flyby are presented. The observations show that the interaction of Jupiter's magnetic field with the solar wind is similar in many ways to that at earth, but the scale size is over 100 times larger. Jupiter is found to have a detached standing bow shock wave of high Alfven Mach number. Jupiter has a prominent magnetopause which deflects the magnetosheath plasma and excludes its direct entry into the Jovian magnetosphere. The sunward hemisphere of Jupiter's outer magnetosphere is found to be highly inflated with thermal plasma and a high beta region which is highly responsive to changes in solar wind dynamic pressure. Observational arguments are presented which tend to discount a thin disklike magnetosphere but, rather, favor a Jovian magnetosphere, albeit probabily considerably flattened as compared to the earth's magnetosphere, yet still with reasonable thickness. Results concerning the shock jump conditions, the magnetosheath flow field and inferred internal magnetospheric plasma are presented.
    Keywords: SPACE RADIATION
    Type: NASA-TM-X-62357
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 9
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    VLBI observations of the nucleus of Centaurus A (1982)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Keywords: ASTROPHYSICS
    Type: Extragalactic radio sources; Aug 03, 1981 - Aug 07, 1981; Albuquerque, NM
    Format: text
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  • 10
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    The thymus-adrenal connection: thymosin has corticotropin-releasing activity in primates (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-12-23
    Description: Endotoxin-free thymosin fraction 5 elevated corticotropin, beta-endorphin, and cortisol in a dose- and time-dependent fashion when administered intravenously to prepubertal cynomolgus monkeys. Two synthetic component peptides of thymosin fraction 5 had no acute effects on pituitary function, suggesting that some other peptides in thymosin fraction 5 were responsible for its corticotropin-releasing activity. In agreement with these observations, total thymectomy of juvenile macaques was associated with decreases in plasma cortisol, corticotropin, and beta-endorphin. These findings indicate that the prepubertal primate thymus contains corticotropin-releasing activity that may contribute to a physiological immunoregulatory circuit between the developing immunological and pituitary-adrenal systems.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Healy, D L -- Hodgen, G D -- Schulte, H M -- Chrousos, G P -- Loriaux, D L -- Hall, N R -- Goldstein, A L -- CA 24974/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1983 Dec 23;222(4630):1353-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6318312" target="_blank"〉PubMed〈/a〉
    Keywords: Adrenocorticotropic Hormone/*blood ; Animals ; Dose-Response Relationship, Drug ; Endorphins/blood ; Female ; Hydrocortisone/blood ; Kinetics ; Macaca fascicularis ; Thymectomy ; Thymosin/analogs & derivatives/*pharmacology ; Thymus Gland/*physiology ; beta-Endorphin
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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