Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine

Science. 1981 Dec 4;214(4525):1125-9. doi: 10.1126/science.6272395.

Abstract

A DNA sequence coding for the immunogenic capsid protein VP3 of foot-and-mouth disease virus A12, prepared from the virion RNA, was ligated to a plasmid designed to express a chimeric protein from the Escherichia coli tryptophan promoter-operator system. When Escherichia coli transformed with this plasmid was grown in tryptophan-depleted media, approximately 17 percent of the total cellular protein was found to be an insoluble and stable chimeric protein. The purified chimeric protein competed equally on a molar basis with VP3 for specific antibodies to foot-and-mouth disease virus. When inoculated into six cattle and two swine, this protein elicited high levels of neutralizing antibody and protection against challenge with foot-and-mouth disease virus.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Formation
  • Base Sequence
  • Cattle
  • Cattle Diseases / prevention & control*
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • DNA, Recombinant / metabolism
  • Foot-and-Mouth Disease / prevention & control*
  • Immunity, Cellular
  • Protein Biosynthesis
  • Swine
  • Swine Diseases / prevention & control*
  • Transcription, Genetic
  • Vaccines*
  • Viral Proteins / genetics
  • Viral Proteins / therapeutic use*

Substances

  • DNA, Recombinant
  • Vaccines
  • Viral Proteins
  • DNA Restriction Enzymes

Associated data

  • GENBANK/J02187