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  • 1
    Publication Date: 2012-09-14
    Description: Deafness is a condition with a high prevalence worldwide, produced primarily by the loss of the sensory hair cells and their associated spiral ganglion neurons (SGNs). Of all the forms of deafness, auditory neuropathy is of particular concern. This condition, defined primarily by damage to the SGNs with relative preservation of the hair cells, is responsible for a substantial proportion of patients with hearing impairment. Although the loss of hair cells can be circumvented partially by a cochlear implant, no routine treatment is available for sensory neuron loss, as poor innervation limits the prospective performance of an implant. Using stem cells to recover the damaged sensory circuitry is a potential therapeutic strategy. Here we present a protocol to induce differentiation from human embryonic stem cells (hESCs) using signals involved in the initial specification of the otic placode. We obtained two types of otic progenitors able to differentiate in vitro into hair-cell-like cells and auditory neurons that display expected electrophysiological properties. Moreover, when transplanted into an auditory neuropathy model, otic neuroprogenitors engraft, differentiate and significantly improve auditory-evoked response thresholds. These results should stimulate further research into the development of a cell-based therapy for deafness.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480718/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480718/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, Wei -- Jongkamonwiwat, Nopporn -- Abbas, Leila -- Eshtan, Sarah Jacob -- Johnson, Stuart L -- Kuhn, Stephanie -- Milo, Marta -- Thurlow, Johanna K -- Andrews, Peter W -- Marcotti, Walter -- Moore, Harry D -- Rivolta, Marcelo N -- 088719/Wellcome Trust/United Kingdom -- G0700785/Medical Research Council/United Kingdom -- G0801059/Medical Research Council/United Kingdom -- G0900919/Medical Research Council/United Kingdom -- G0900919(92659)/Medical Research Council/United Kingdom -- G20/Action on Hearing Loss/United Kingdom -- G34/Action on Hearing Loss/United Kingdom -- G44/Action on Hearing Loss/United Kingdom -- Medical Research Council/United Kingdom -- England -- Nature. 2012 Oct 11;490(7419):278-82. doi: 10.1038/nature11415. Epub 2012 Sep 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Centre for Stem Cell Biology, University of Sheffield, Sheffield S10 2TN, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22972191" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Auditory Threshold ; *Cell Differentiation ; Cell Line ; Cells, Cultured ; Cochlear Nerve/cytology/physiology ; Deafness/chemically induced/therapy ; Embryonic Stem Cells/*cytology ; *Evoked Potentials, Auditory ; Fibroblast Growth Factor 10/genetics/metabolism ; Fibroblast Growth Factor 3/genetics/metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gerbillinae ; Hair Cells, Auditory/cytology/physiology ; Humans ; Mice ; Patch-Clamp Techniques ; Stem Cell Transplantation ; Stem Cells/*cytology
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 1995-07-07
    Description: A 95-kilodalton mouse sperm protein with characteristics of a protein tyrosine kinase has been identified as a receptor for ZP3, a glycoprotein in the egg's extracellular matrix. The structure of the human homolog was determined by screening an expression library from human testis; a testis-specific complementary DNA was isolated that encodes a protein similar to receptor tyrosine kinases and appears to be expressed only in testicular germ cells. Antibodies against a synthetic peptide from the intracellular domain recognized a 95-kilodalton human sperm protein that contains phosphotyrosine; human ZP3 stimulates the kinase activity of this sperm protein. Synthetic peptides corresponding to regions of the predicted extracellular domain inhibited sperm binding to human zona pellucida. Availability of the primary sequence of a receptor for ZP3 provides a rational starting point for sperm-targeted contraceptive development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Burks, D J -- Carballada, R -- Moore, H D -- Saling, P M -- HD 18201/HD/NICHD NIH HHS/ -- HD 29125/HD/NICHD NIH HHS/ -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 1995 Jul 7;269(5220):83-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC 27710, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7541556" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Egg Proteins/*metabolism ; Female ; Humans ; Male ; Membrane Glycoproteins/*metabolism ; Mice ; Molecular Sequence Data ; Molecular Weight ; Phosphorylation ; Phosphotyrosine ; Proto-Oncogene Proteins ; Receptor Protein-Tyrosine Kinases/chemistry/genetics/*metabolism ; *Receptors, Cell Surface ; Sperm-Ovum Interactions/*physiology ; Spermatozoa/*metabolism ; Tyrosine/analogs & derivatives/metabolism ; Zona Pellucida/*metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1984-03-16
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉O'Brien, S J -- Goldman, D -- Knight, J -- Moore, H D -- Wildt, D E -- Bush, M -- Montali, R J -- Kleiman, D -- New York, N.Y. -- Science. 1984 Mar 16;223(4641):1127-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6701515" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Carnivora ; Male ; *Paternity ; Polymorphism, Genetic ; Proteins/analysis
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    Publication Date: 2016-06-07
    Description: The temporal changes that are recorded by ERTS were evaluated for an area around Bathurst Inlet in the North West Territories. The seasons represented by the images included: early winter, spring, early summer, summer, and fall. Numerous surface characteristics (vegetation, drainage patterns, surface texture, lineament systems and topographic relief, etc.) were used to relate the change in observable features with the different seasons. It was found that the time of year when an observation is made has a strong control over the amount and type of information that can be derived by an experienced interpreter. It was concluded that a detailed study of temporal changes is an important part of any ERTS interpretation for geology.
    Keywords: GEOPHYSICS
    Type: PAPER-G20 , NASA. Goddard Space Flight Center 3d ERTS-1 Symp., Vol. 1, Sect. A; p 845-855
    Format: application/pdf
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  • 5
    ISSN: 1040-452X
    Keywords: Stallion spermatozoa ; Acrosome reaction ; Monoclonal antibody ; Zona-free hamster eggs ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The acrosome of the stallion spermatozoon was visualized by indirect immunofluorescence with monoclonal antibody (18.6) which recognized an integral acrosomal membrane component. Localization was confirmed by electron microscopy using peroxidase labelled antibody. In fresh semen samples (n = 19), 73.9 ± 9.1% of the spermatozoa from five fertile stallions displayed a uniform bright fluorescence over their acrosome region. In two semen samples from an infertile stallion only 28% and 35% of spermatozoa showed the same pattern of fluorescence. Spermatozoa from fertile stallions incubated for up to 12 hours in TALP medium maintained motility and exhibited a significant progressive loss of acrosomes as detected by immunofluorescence. Alternatively, a similar loss of acrosomes could be induced with calcium ionophore A23187 over a 90 minute incubation. Ultrastructural observations and incubation with zona-free hamster eggs indicated that only with ionophore treatment was immunofluorescent acrosome loss correlated with a physiological acrosome reaction, while prolonged sperm incubation led to degenerative membrane changes. It was concluded that, if carefully validated, immunofluorescent localization of the acrosome of stallion sperm with monoclonal antibody could be used to monitor the acrosome reaction. Furthermore, definitive acrosome visualization would be valuable in assessing semen quality.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 41 (1995), S. 348-354 
    ISSN: 1040-452X
    Keywords: Spermatogenesis ; Germ cells ; Immunoselection ; Monoclonal antibody ; RNA ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A method was devised for the isolation of round spermatids from the rat using a positive immunoselection technique (panning). A testis suspension was prepared from adult rats by enzymatic digestion of seminiferous tubules with collagenase. Specific mouse monoclonal antibody (97.25) was indirectly attached to Petri dishes and used in a panning protocol to purify spermatids from the testis cell suspension. The quantity and purity of cells isolated were determined by cell counts and histochemical (periodic acid-Schiff stain) or by immunostaining with acrosome-specific antibodies. A mean yield of 1.38 ± 0.15 × 107 cells per dish was obtained with a purity of more than 90%. The viability of the cells was confirmed by epifluorescent microscopy with propidium iodide/carboxyfluorescein acetate probes. Northern blot analysis of RNA extracted directly from the dish indicated good integrity of a spermatid-specific transcript of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
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  • 7
    ISSN: 0148-7280
    Keywords: hamster ova ; epi-fluorescence ; electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A monoclonal antibody generated against hamster epididymal spermatozoa and recognizing an antigen within the acrosome was used in conjunction with FITC-antimouse immunoglobulin as a marker of the human acrosome during sperm development, capacitation, and the acrosome reaction. The specificity of binding of the monoclonal antibody was assessed using immunolocalization by epi-fluorescence and electron microscopy. Immunofluorescence revealed that antibody bound over the entire anterior acrosome in hamster and human spermatozoa. Ultrastructural localization indicated that antigen was predominantly present on the inner face of the outer acrosomal membrane and within the acrosomal content. Qualitative specificity was studied using a highly purified preparation of hamster acrosomes in an enzyme-linked immunosorbent assay. Since the antibody rapidly visualized human acrosomes, it was used to detect abnormal acrosome morphology of mature spermatozoa and to mark spermatids present in the ejaculate. During incubation in capacitating medium, changes in the immunofluorescence of live or methanol fixed spermatozoa were correlated with incubation interval and the ability of spermatozoa to fuse with zona-free hamster oocytes. Spermatozoa bound to zona-free hamster oocytes displayed no fluorescence, confirming that acrosome loss occurred before spermatozoa attached to the vitellus.
    Additional Material: 14 Ill.
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  • 8
    Publication Date: 2008-01-14
    Description: Reversible ring counter using cascaded single silicon controlled rectifier stages
    Keywords: ELECTRONIC EQUIPMENT
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  • 9
    Publication Date: 2019-05-21
    Description: A power logic circuit, with bidirectional capability, is used to drive small loads in planned sequence. This is designed in the form of a shift register, with a reversible ring counter.
    Keywords: ELECTRONIC COMPONENTS AND CIRCUITS
    Type: GSC-101
    Format: application/pdf
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  • 10
    Publication Date: 2018-12-21
    Description: Author(s): B. Bucher, H. Mach, A. Aprahamian, L. M. Robledo, G. S. Simpson, J. Rissanen, D. G. Ghiţă, B. Olaizola, W. Kurcewicz, J. Äystö, T. Eronen, L. M. Fraile, A. Jokinen, P. Karvonen, I. D. Moore, H. Penttilä, M. Reponen, E. Ruchowska, A. Saastamoinen, M. K. Smith, and C. Weber Rapid shape evolutions near A = 100 are now the focus of much attention in nuclear science. Much of the recent work has been centered on isotopes with Z ≤ 40 , where the shapes are observed to transition between near-spherical to highly deformed with only a single pair of neutrons added. At higher Z , the... [Phys. Rev. C 98, 064320] Published Thu Dec 20, 2018
    Keywords: Nuclear Structure
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
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