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  • Protoplast fusion  (2)
  • plant regeneration
  • 1990-1994  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of potato-tomato somatic hybrids using an amylose-free potato mutant as parental genotype (1992)
    Springer
    Theoretical and applied genetics 85 (1992), S. 159-164 
    Details
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; Lycopersicon esculentum ; Protoplast fusion ; Amylose-free potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using different genotypes of tomato and diploid potato, possessing alien selectable markers as well as endogenous markers, very high frequencies of protoplast fusion hybrids were obtained. One endogenous genetic marker, the amylose-free (amf) mutant of potato, was helpful not only for the confirmation of fusion products but also for the study of genetic complementation and the segregation of amylose-free starch in microspores. Cytological analysis of the fusion hybrids indicated that except for one which was hexaploid, all of them had a perfectly balanced chromosome number of allotetraploid constitution (2n = 4x = 48). Despite normal chromosome pairing and a diploid behaviour, the microspores in some of the fusion hybrids segregated for the recessive amf-locus. This anomalous segregation of a recessive character in these hybrids was shown not to be due to chromosome elimination or to the absence of the wild-type tomato Amf gene. Although all fusion hybrids were totally sterile, the hexaploid produced stainable pollen and berries with badly developed seeds. Embryo rescue has so far failed to produce backcross progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222854
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  • 2
    Electronic Resource
    Electronic Resource
    The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 181-186 
    Details
    ISSN: 1432-2242
    Keywords: Protoplast fusion ; Potato ; Tomato ; Post-fertilisation barriers ; Introgression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic fusion hybrids between the diploid potato and tomato were backcrossed to several genotypes of potato. Two ploidy levels of fusion hybrids, 4x and 6x, were used as female parents in backcrosses with five clones of 4x-potato. An estimate of the berry set and “seed set” in immature berries harvested 14–21 days after pollination indicated that crosses between certain combinations of 6x-fusion hybrids and male parents were more successful than others. The culture of over 4000 young seeds from berries harvested 2–2.5 weeks after pollination gave rise to a single seedling, 93.6701, from the cross between the 6x-fusion hybrid C 31-17-1 and the 4x-potato AM 66.42. This seedling was found to possess a pentaploid chromosome number, which was expected of a 6x × 4x cross. Isozyme analysis and DNA hybridisation studies confirmed that the seedling 93.6701 was indeed a backcross (BC1) progeny. Morphologically, this BC1 plant resembled potato with respect to plant habit, leaf shape, stolons and tuber characteristics, while some of the characters, such as floral morphology and the fragrance of the crushed leaves (typical of tomato), were intermediate. It was male sterile but could be successfully hybridized with 4x-potato through in vitro culture of yound seeds; thus, BC2 plants were obtained. The possibilities of backcrossing and the potential use of BC1 and BC2 plants in genetics and breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225895
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  • 3
    Electronic Resource
    Electronic Resource
    Induction, germination and shoot development of somatic embryos in cassava (1993)
    Springer
    Plant cell, tissue and organ culture 33 (1993), S. 151-156 
    Details
    ISSN: 1573-5044
    Keywords: cassava ; plant regeneration ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four Indonesian and two Latin-American cassava genotypes (Manihot esculenta Crantz), were evaluated for their ability to develop somatic embryos from young leaf lobes. All genotypes formed somatic embryos but they differed in the frequency of embryos induced. The best genotypes, M. Col 22 and Tjurug, produced germinating embryos (GE) on 81% (22.1 GE/initial leaf lobe) and 46% (4.3 GE/initial leaf lobe) of the cultured leaf lobes, respectively. Up to 57% of the germinating embryos of M. Col 22 and 12% of Tjurug produced either normal or malformed shoots. Most malformed shoots developed into shoots with normal morphology after prolonged culture. All shoots formed roots after transfer to medium without BAP. Roots of all normal and most malformed regenerants had the original ploidy level (2n=36). Regardless of whether the plants were multipliedin vitro (150 plants) or in the greenhouse (30 plants) there were no morphological differences compared to parent plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01983228
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