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  • Oryza sativa  (34)
  • Springer  (34)
  • Blackwell Publishing Ltd
  • International Union of Crystallography
  • 1995-1999  (34)
  • 1960-1964
  • 1925-1929
Collection
Keywords
Publisher
  • Springer  (34)
  • Blackwell Publishing Ltd
  • International Union of Crystallography
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Micropropagation of Indica rice through proliferation of axillary shoots (1995)
    Springer
    Euphytica 81 (1995), S. 139-142 
    Details
    ISSN: 1573-5060
    Keywords: Oryza sativa ; indica rice ; shoot proliferation ; micropropagation ; cytokinins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Week old seedlings of indica rice variety Jaya obtained on basal MS medium and further sub-cultured on agar solidified MS medium supplemented with cytokinins, sucrose (3% w/v) and mannitol (1% w/v) lead to development of multiple shoot buds. Shoot cultures were maintained and multiplied in liquid medium containing BAP 5 mg l-1, sucrose (3% w/v) and mannitol (1% w/v). Profuse rooting was obtained on transfer to MS liquid medium containing IBA 1 mg l-1 and sucrose (3% w/v). Complete plants were successfully transferred to soil and grown to maturity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025425
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  • 2
    Electronic Resource
    Electronic Resource
    Chromosomal location of fertility restoring genes for ‘wild abortive’ cytoplasmic male sterility using primary trisomics in rice (1995)
    Springer
    Euphytica 83 (1995), S. 169-173 
    Details
    ISSN: 1573-5060
    Keywords: rice ; Oryza sativa ; cytoplasmic male sterility ; fertility restoration ; chromosomal gene location ; primary trisomics ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Identification and location of fertility restoring genes facilitates their deployment in a hybrid breeding program involving cytoplasmic male sterility (CMS) system. The study aimed to locate fertility restorer genes of CMSWA system on specific chromosomes of rice using primary trisomics of IR36 (restorer), CMS (IR58025A) and maintainer (IR58025B) lines. Primary trisomic series (Triplo 1 to 12) was crossed as maternal parent with the maintainer line IR58025B. The selected trisomic and disomic F1 plants were testcrossed as male parents with the CMS line IR58025A. Plants in testcross families derived from disomic F1 plants (Group I crosses) were all diploid; however, in the testcross families derived from trisomic F1 plants (Group II crosses), some trisomic plants were observed. Diploid plants in all testcross families were analyzed for pollen fertility using 1% IKI stain. All testeross families from Group I crosses segregated in the ratio of 2 fertile: 1 partially fertile+partially sterile: 1 sterile plants indicating that fertility restoration was controlled by two independent dominant genes: one of the genes was stronger than the other. Testcross families from Group II crosses segregated in 2 fertile: 1 partially fertile+ partially sterile: 1 sterile plants in crosses involving Triplo 1, 4, 5, 6, 8, 9, 11 and 12, but families involving triplo 7 and triplo 10 showed significantly higher X2 values, indicating that the two fertility restorer genes were located on chromosome 7 and 10. Stronger restorer gene (Rf-WA-1) was located on chromosome 7 and weaker restorer gene (Rf-WA-2) was located on chromosome 10. These findings should facilitate tagging of these genes with molecular markers with the ultimate aim to practice marker-aided selection for fertility restoration ability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01678126
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  • 3
    Electronic Resource
    Electronic Resource
    Classification of rice germplasm: III. High-resolution fingerprinting of cytoplasmic genetic male-sterile (CMS) lines with AFLP (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 941-949 
    Details
    ISSN: 1432-2242
    Keywords: Key words AFLP ; Fingerprinting ; Genetic diversity ; Hybrid rice ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The cytoplasmic genetic male-sterile (CMS) lines developed at the International Rice Research Institute are valuable in producing tropical rice hybrids. Efficient use of CMS lines in hybrid rice production will depend on their level of genetic diversity. Aside from morphological characterization, molecular analysis based on DNA markers can provide information on the genetic diversity of the germplasm. The Amplified Fragment Length Polymorphism (AFLP) technique was used to fingerprint 71 CMS lines and four rice cultivars, ‘IR64’, ‘Azucena’, ‘IR74’, and ‘FR13A’. Eleven primer pair combinations specific to the enzymes PstI and MseI were used to generate 530 AFLP markers, 176 of which were polymorphic. Each CMS line revealed a distinct fingerprint. The AFLP marker-based dendrogram depicted genetic variation among the CMS lines. The CMS lines developed in japonica background grouped with ‘Azucena’, a japonica cultivar. None of the CMS lines clustered with ‘FR13A’, a flood-tolerant traditional indica variety. ‘IR64’ was found to be distinct from the other indica CMS lines and clustered with lines developed in its background. The grouping of CMS lines into a few groups is useful for breeders in selecting genetically diverse CMS lines for hybrid rice production and in avoiding test crossing every CMS line empirically. This study demonstrated that AFLP is a powerful and reliable tool in determining the genetic relationships and in producing distinct fingerprints of rice cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050824
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  • 4
    Electronic Resource
    Electronic Resource
    Drought-induced male sterility in rice: Changes in carbohydrate levels and enzyme activities associated with the inhibition of starch accumulation in pollen (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 161-169 
    Details
    ISSN: 1432-2145
    Keywords: Oryza sativa ; Pollen sterility ; Water stress ; Starch biosynthesis ; Sugar metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Male reproductive development of rice (Oryza sativa L.) is very sensitive to drought. A brief, transitory episode of water stress during meiosis in pollen mother cells of rice grown under controlled environmental conditions induced pollen sterility. Anthers containing sterile pollen were smaller, thinner, and often deformed compared to normal anthers of well-watered plants. Only about 20% of the fully developed florets in stressed plants produced grains, compared to 90% in well-watered controls. Water stress treatments after meiosis were progressively less damaging. Levels of starch and sugars and activities of key enzymes involved in sucrose cleavage and starch synthesis were analyzed in anthers collected at various developmental stages from plants briefly stressed during meiosis and then re-watered. Normal starch accumulation during pollen development was strongly inhibited in stress-affected anthers. During the period of stress, both reducing and non-reducing sugars accumulated in anthers. After the relief of stress, reducing sugar levels fell somewhat below those in controls, but levels of non-reducing sugars remained higher than in controls. Activities of acid invertase and soluble starch synthase in stressed anthers were lower than in controls at comparable stages throughout development, during as well as after stress. Stress had no immediate effect on ADP-glucose pyrophosphorylase activity, but had an inhibitory aftereffect throughout post-stress development. Sucrose synthase activity, which was, relatively speaking, much lower than acid invertase activity, was only slightly suppressed by stress. The results show that it is unlikely that pollen sterility, or the attendant inhibition of starch accumulation, in water-stressed rice plants are caused by carbohydrate starvation per se. Instead, an impairment of enzymes of sugar metabolism and starch synthesis may be among the potential causes of this failure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221396
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  • 5
    Electronic Resource
    Electronic Resource
    DNA markers tightly linked to a gall midge resistance gene (Gm2) are potentially useful for marker-aided selection in rice breeding (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 68-73 
    Details
    ISSN: 1432-2242
    Keywords: PCR ; RAPDs ; Oryza sativa ; Insect resistance ; Marker-aided selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed a polymerase chain reaction (PCR)-based assay that could effectively reduce the time period required to screen and select for Gall Midgeresistant rice lines under field conditions. The primers for the assay were designed on the basis of sequence information of two phenotype specific random amplified polymorphic DNA fragments which were found to be tightly linked to Gall Midge biotype-1 resistance gene (Gm2). The two RAPD fragments, F81700 in the susceptible parent ‘ARC6650’ and F10600 in the resistant parent ‘Phalguna’, were identified after screening 5450 loci using 520 random primers on genomic DNAs of ‘ARC6650’ and ‘Phalguna’. These primers, when used in a multiplexed PCR, amplified specifically a 1.7-kb and 0.6-kb fragment in the susceptible and resistant parents, respectively. When this assay was performed on genomic DNAs of 44 recombinant inbred lines derived from ‘ARC6650’ x ‘Phalguna’ and 5 lines derived from other crosses where one of the parents was ‘Phalguna’, ‘ARC6650’ or their derivatives, the primers amplified a 1.7-kb fragment in all of the susceptible lines or a 0.6-kb fragment in all of the resistant ones. These markers can be of potential use in the marker-aided selection of Gall Midge biotype-1 resistant phenotypes. As screening for resistance can now be conducted independent of the availability of insects, the breeding of resistant varieties can be hastened.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220860
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  • 6
    Electronic Resource
    Electronic Resource
    Anthocyanin pathway in rice (Oryza sativa L): identification of a mutant showing dominant inhibition of anthocyanins in leaf and accumulation of proanthocyanidins in pericarp (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 301-312 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa ; Inhibitor ; Anthocyanins ; Proanthocyanidins ; Pigmentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The present study has surveyed a collection of indica rice (Oryza sativa) lines for tissue-specific anthocyanin pigmentation pattern, which has also been used for a genetically meaningful classification. This classification helped predict probable genotypes of rice lines and, in the process, a leaf blade-specific dominant inhibitor of pigmentation (Ilb) was predicted and its presence later confirmed in two lines. We ascribe most tissue-specific accumulation of anthocyanins to the presence of a different set of Pl alleles. Cyanidin, as a major pigment, and peonidin, as a minor pigment, were detected in purple-pigmented tissues. Further, the floral organ-derived tissues always contained a higher level of anthocyanins and, correspondingly, a relatively increased proportion of peonidin. One line, N22B, with a brown pericarp was identified and shown to accumulate proanthocyanidins, but with no anthocyanins, in the pericarp. We propose that the accumulation of proanthocyanidins is due to a block in the anthocyanin biosynthetic pathway in rice at the anthocyanidin synthase-mediated conversion of leucoanthocyanidin to anthocyanidin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220892
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  • 7
    Electronic Resource
    Electronic Resource
    A gene encoding a truncated large subunit of Rubisco is transcribed and salt-inducible in rice (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 361-366 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa ; Rice salt-tolerant mutant ; Chimeric rbcL ; Rubisco ; Salt stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using the rice salt-tolerant mutant 20 as material, a cDNA library was constructed and two salt-inducible clones, SIR5.5 and SIR8.1, were isolated by differential screening. Homology analysis revealed that the two clones together constituted a chimeric rbcL which encoded a truncated large subunit of Rubisco with 337 amino-acids, plus 64 amino-acids of unknown origin. The expressions of both the normal and the chimeric locus appeared to be developmentally regulated and salt-inducible in shoots of the salt-tolerant mutant 20 and its original variety 77–170. In roots, their expressions were salt-inducible in the salt-tolerant mutant 20 whereas no, or only premature, forms were present in the salt-treated original variety 77–170. Higher concentrations of salt reduced the expressions of both normal rbcL and the chimeric locus. ABA showed no effect on their expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220900
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular analysis of the inheritance of the S-5 locus, conferring wide compatibility in Indica/Japonica hybrids of rice (O. sativa L.) (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 182-188 
    Details
    ISSN: 1432-2242
    Keywords: Wide compatibility ; Oryza sativa ; RFLP markers ; Hybrid sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RFLP analysis was conducted on a population derived from a three-way cross to determine the location of the hybrid sterility locus, S-5, in relation to mapped molecular markers and to identify markers that would be useful for selection in breeding. S-5 is of interest to rice breeders because it is associated with spikelet sterility of F1 hybrids in Indica/Japonica crosses. Identification of an S-5 allele which confers fertility in Indica/Japonica hybrids when introgressed into either the Indica or the Japonica parent has been reported. Varieties carrying this S-5 n allele are known as “wide compatibility varieties (WCV)”. Our data suggests that RFLP marker RG213 on chromosome 6 is closely linked to the S-5 locus and can be efficiently used to identify wide compatibility (WC) lines. RG213 is a single-copy genomic clone that detects three bands of different molecular weights in DNA from Japonica (‘Akihikari’) and Indica (‘IR36’) varieties and WC line (‘Nekken 2’). We demonstrate that the three alleles detected by this marker could be used to trace the inheritance of the “wide compatible” phenotype in breeders' material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222200
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  • 9
    Electronic Resource
    Electronic Resource
    Genetics of thermosensitive genic male sterility in rice (1996)
    Springer
    Euphytica 88 (1996), S. 1-7 
    Details
    ISSN: 1573-5060
    Keywords: rice ; Oryza sativa ; hybrid rice ; thermosensitive genic male sterility (TGMS) ; rice genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Inheritance of thermosensitive genic male sterility (TGMS) in Norin PL12 and IR32364TGMS and their allelic relationship were studied from F1, F2 testcross (TC) and F3 generations of the crosses made with the two mutants and several fertile tester parents. F2, TC and F3 segregation behavior for pollen and spikelet fertility indicated that the TGMS trait in the two mutants was controlled by a single recessive gene. Allelic relationship studies indicated that TGMS genes of the two mutants were different. Since TGMS gene in Norin PL12 has been designated as tms 2 , the TGMS gene present in IR32364TGMS is tentatively designated as tms 3 (t) until allelic test is done with another TGMS gene (tms 1 ) reported from China in a line 5460S seeds of which were not available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029259
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  • 10
    Electronic Resource
    Electronic Resource
    Methane emission from two Indian soils planted with different rice cultivars (1997)
    Springer
    Biology and fertility of soils 25 (1997), S. 285-289 
    Details
    ISSN: 1432-0789
    Keywords: Key words Methane emission ; Wetland soils ; Greenhouse gases ; Inceptisol ; Vertisol ; Rice ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a greenhouse study, methane emissions were measured from two diverse Indian rice-growing soils planted to five rice cultivars under similar water regimes, fertilizer applications and environmental conditions. Significant variations were observed in methane emitted from soils growing different cultivars. Total methane emission varied between 8.04 and 20.92gm–2 from IARI soil (Inceptisol) and between 1.47 and 10.91gm–2 from Raipur soil (Vertisol) planted to rice. In all the cultivars, emissions from IARI soil were higher than from Raipur soil. The first methane flux peak was noticed during the reproductive phase and the second peak coincided with the grain-ripening stage of the rice cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050316
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