ISSN:
1573-5060
Keywords:
Oryza sativa
;
indica rice
;
shoot proliferation
;
micropropagation
;
cytokinins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Week old seedlings of indica rice variety Jaya obtained on basal MS medium and further sub-cultured on agar solidified MS medium supplemented with cytokinins, sucrose (3% w/v) and mannitol (1% w/v) lead to development of multiple shoot buds. Shoot cultures were maintained and multiplied in liquid medium containing BAP 5 mg l-1, sucrose (3% w/v) and mannitol (1% w/v). Profuse rooting was obtained on transfer to MS liquid medium containing IBA 1 mg l-1 and sucrose (3% w/v). Complete plants were successfully transferred to soil and grown to maturity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00025425