Summary
Week old seedlings of indica rice variety Jaya obtained on basal MS medium and further sub-cultured on agar solidified MS medium supplemented with cytokinins, sucrose (3% w/v) and mannitol (1% w/v) lead to development of multiple shoot buds. Shoot cultures were maintained and multiplied in liquid medium containing BAP 5 mg l-1, sucrose (3% w/v) and mannitol (1% w/v). Profuse rooting was obtained on transfer to MS liquid medium containing IBA 1 mg l-1 and sucrose (3% w/v). Complete plants were successfully transferred to soil and grown to maturity.
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Sandhu, J.S., Gosal, S.S., Gill, M.S. et al. Micropropagation of Indica rice through proliferation of axillary shoots. Euphytica 81, 139–142 (1995). https://doi.org/10.1007/BF00025425
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DOI: https://doi.org/10.1007/BF00025425