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  • Image analysis  (5)
  • Springer  (5)
  • 2005-2009
  • 1990-1994  (5)
  • 1
    Electronic Resource
    Electronic Resource
    High resolution bands in maize chromosomes by G-banding methods (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 265-272 
    Details
    ISSN: 1432-2242
    Keywords: Maize ; Chromosome banding ; G-banding method ; Enzymatic maceration ; Image analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary It was demonstrated that G-bands are unequivocally present in plant chromosomes, in contrast to what had been formerly believed by plant cytologists. Maize chromosomes prepared by an enzymatic maceration method and treated with trypsin or SDS showed clear G-bands spreading along the chromosomes. The most critical point during the G-banding procedures was the post-fixation with glutaraldehyde solution. Banding patterns were processed by using the chromosome image analyzing system and a clearer image was obtained. Gbanding technique and the image manipulation method described here can be applied to many plant species, and would contribute new information in the field of plant cytology and genetics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224397
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  • 2
    Electronic Resource
    Electronic Resource
    Microdissection of plant chromosomes by argon-ion laser beam (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 787-791 
    Details
    ISSN: 1432-2242
    Keywords: Barley chromosomes ; Rice chromosomes ; Laser dissection ; Micromanipulation ; Image analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rice and barley chromosomal samples were prepared both on a polyester membrane and on an ordinary glass slide and subjected to microdissection by an argon-ion laser. The intensity and the position of the laser beam were controlled by a microcomputer. The most suitable intensity to obtain chromosomal fragments was determined experimentally. As a result, specific regions of the centromere, satellite, short arm, or long arm, of the barley and rice chromosomes were dissected out from the chromosomal spreads. Chromosomal fragments were also successfully transferred from the sample into an Eppendorf tube.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227385
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  • 3
    Electronic Resource
    Electronic Resource
    Variability in rDNA loci in the genus Oryza detected through fluorescence in situ hybridization (1994)
    Springer
    Theoretical and applied genetics 87 (1994), S. 893-899 
    Details
    ISSN: 1432-2242
    Keywords: Fluorescence in-situ hybridization (FISH) ; Ribosomal DNA ; Genus Oryza ; Image analysis ; NOR variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 17s-5.8s-25s ribosomal RNA gene (rDNA) loci in Oryza spp. were identified by the fluorescence in-situ hybridization (FISH) method. The rDNA loci were located on one-to-three chromosomes (two-to-six sites) within the eight diploid Oryza spp. One of the rDNA loci gave the weakest hybridization signal. This locus is reported for the first time in the genus Oryza. The chromosomes containing the rDNA loci were determined to be numbers 9, 10 and 11 in descending order of the copy number of rDNA. The application of image analysis methods, after slide preparation treatments (post-treatments), and the use of a thermal cycler, greatly improved the reproducibility of the results. The evolutionary significance of the variability of rDNA loci among the Oryza spp. is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225782
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  • 4
    Electronic Resource
    Electronic Resource
    Somatic chromosome map of rice by imaging methods (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 589-596 
    Details
    ISSN: 1432-2242
    Keywords: Rice ; Chromosome map ; Ideogram ; Image analysis ; Condensation pattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rice somatic chromosomes were completely identified and quantitatively mapped based on an image parameter, condensation pattern (CP), or a chromosomal density profile determined by imaging methods. The CP corresponds to the compactness of the chromatin fibers along the chromatid, which is characteristic in small plant chromosomes such as rice chromosomes at the mitotic pro-metaphase stage. The standard CP for every chromosome was obtained by averaging 60 CPs from 30 chromosome spreads. Each standard CP exhibited a characteristic pattern of the chromosome, which enabled it to be distinguished from the other chromosomes. An ideogram based on the numerical data and the standard CP was established. The chromosomal address was also determined based on the degree of condensation, and the fractional length of each chromosomal address was quantitatively presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226723
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  • 5
    Electronic Resource
    Electronic Resource
    Marker chromosomes commonly observed in the genus Glycine (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 606-612 
    Details
    ISSN: 1432-2242
    Keywords: Genus Glycine ; Soybean ; Karyotype ; Marker chromosome ; Image analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Soybean [Glycine max (L.) Merr.] chromosomes were analyzed using the chromosome image analyzing system, CHIAS, and seven groups, including subgroups, were identified based on morphological characteristics. Two pairs of chromosomes were conspicuous in their morphological traits. One pair of chromosomes, which had the largest arm ratio among all the chromosomes, was commonly observed in the species in all three subgenera of the genus Glycine. These chromosomes also displayed a unique pattern after N-banding and were detected as marker chromosomes. G. soja, which is considered to be the ancestor of G. max, has two types of marker chromosomes. The lines that carry the same type as G. max may be the ancestors of G. max among the lines of G. soja. The morphological differences of the marker chromosomes within the species in the subgenus Soja are discussed in relation to the domestication process of soybean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226725
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