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  • 1
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    Establishment of gene bank of inland water fish species (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-12-05
    Description: Genetic knowledge helps to protect biodiversity and optimal harvest resources by several ways. This knowledge can help to reduce the risk of extinction to those populations that have high genetic diversity or diminished by detected them. Also, studies of population genetics can present guidelines for improving the structure of the population and understanding the biology of species. One of the most important applications of DNA database is detecting species, fishing offense, diagnose of anemia and genetic classification of animals. DNA bank has helped to identify the species that are hunted and were discovered only parts of their meat and texture. Optimal Storage of aquatic genetic resources and the conservation of biological diversity, as the national capital by using biotechnology methods are the most important goals of gene bank and database reserves of the country creation.Collecting biological specimens of aquatic species, preparation and long-term maintenance of them, genetic registration of endangered and threatened species and the use of biotechnology techniques for the protection, conservation and management of aquatic genetic resources is one of the other functions of the gene bank. Gene bank of inland waters of Iran (Gorgan) is planned and implemented to identify, collect, maintain and protect species at the level of genes, cells, tissues and live fish research and commercial exploitation. Despite the predicted projected funding for this project, unfortunately funding sources have been considered, does not allocated. Therefore some parts of the aims of this project has been organized by Golestan province credits to build molecular laboratory and aquaculture pond in Alang farm and exploitation of potential Gharasou station and Sijoval center restocking and the conditions for the bulk of the project goals is Provided. Some parts like tissue culture laboratory and bioinformatics as well as parts and equipment related to the freezing of sperm and embryo development are the items that should be considered in the development of the this bank.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: GenBank ; Gene Bank ; Fish ; Species ; Genetic ; Biotechnology
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 74pp.
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  • 2
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    Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance (2016)
    Nature Publishing Group (NPG)
    Details
    Publication Date: 2016-04-28
    Description: The Bacillus thuringiensis delta-endotoxins (Bt toxins) are widely used insecticidal proteins in engineered crops that provide agricultural, economic, and environmental benefits. The development of insect resistance to Bt toxins endangers their long-term effectiveness. Here we have developed a phage-assisted continuous evolution selection that rapidly evolves high-affinity protein-protein interactions, and applied this system to evolve variants of the Bt toxin Cry1Ac that bind a cadherin-like receptor from the insect pest Trichoplusia ni (TnCAD) that is not natively bound by wild-type Cry1Ac. The resulting evolved Cry1Ac variants bind TnCAD with high affinity (dissociation constant Kd = 11-41 nM), kill TnCAD-expressing insect cells that are not susceptible to wild-type Cry1Ac, and kill Cry1Ac-resistant T. ni insects up to 335-fold more potently than wild-type Cry1Ac. Our findings establish that the evolution of Bt toxins with novel insect cell receptor affinity can overcome insect Bt toxin resistance and confer lethality approaching that of the wild-type Bt toxin against non-resistant insects.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865400/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865400/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Badran, Ahmed H -- Guzov, Victor M -- Huai, Qing -- Kemp, Melissa M -- Vishwanath, Prashanth -- Kain, Wendy -- Nance, Autumn M -- Evdokimov, Artem -- Moshiri, Farhad -- Turner, Keith H -- Wang, Ping -- Malvar, Thomas -- Liu, David R -- R01 EB022376/EB/NIBIB NIH HHS/ -- R01EB022376/EB/NIBIB NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 2016 Apr 27.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA. ; Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA. ; Monsanto Company, 245 First Street, Suite 200, Cambridge, Massachusetts 02142, USA. ; Department of Entomology, Cornell University, Geneva, New York 14456, USA. ; Monsanto Company, 700 Chesterfield Parkway West, Chesterfield, Missouri 63017, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/27120167" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Bacillus thuringiensis/*genetics ; Bacterial Proteins/*genetics/*metabolism ; Bacteriophages/genetics ; Biotechnology ; Cadherins/metabolism ; Cell Death ; Consensus Sequence ; Crops, Agricultural/genetics/metabolism ; Directed Molecular Evolution/*methods ; Endotoxins/*genetics/*metabolism ; Genetic Variation/*genetics ; Hemolysin Proteins/*genetics/*metabolism ; *Insecticide Resistance ; Insecticides/metabolism ; Molecular Sequence Data ; Moths/cytology/*physiology ; Mutagenesis/genetics ; Pest Control, Biological/*methods ; Plants, Genetically Modified ; Protein Binding/genetics ; Protein Stability ; Selection, Genetic
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Published by Nature Publishing Group (NPG)
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  • 3
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    National roadmap for aquatic organisms biological products (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Specialists in Marine biotechnology using marine biological engineering and scientific principles to develop natural products and regarded services. One of the most important applications of this new branch is produce the natural products such as enzymes, natural colors, vaccines and drugs and etc. which produced from marine living organisms. These products are used in various industries such as pharmaceutical, food or health industry. In the current study, we try to remark the application of marine biological products in the northern (Caspian Sea), southern (Persian Gulf and Oman Sea) or internal waters of Iran into six categories, including aquaculture, pharmaceutical industry, food, healthcare and cosmetic industries. For this purpose, the required information will be collected using investigations which conducted inside and also outside of our country by reviewing and analysis of derived data by attention to our economical facilities for national self-sufficiency in production. Applied research projects will be introduced in three parts including research, technology development and pilot plant as well. Hope that using this strategic document will be effective and practical to production of biological products from marine resources until 2025.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Enzymes ; Marine ; Biological ; Biotechnology ; Aquatic
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 131pp.
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  • 4
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    Designer protein delivery: From natural to engineered affinity-controlled release systems (2016)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2016-03-19
    Description: Exploiting binding affinities between molecules is an established practice in many fields, including biochemical separations, diagnostics, and drug development; however, using these affinities to control biomolecule release is a more recent strategy. Affinity-controlled release takes advantage of the reversible nature of noncovalent interactions between a therapeutic protein and a binding partner to slow the diffusive release of the protein from a vehicle. This process, in contrast to degradation-controlled sustained-release formulations such as poly(lactic-co-glycolic acid) microspheres, is controlled through the strength of the binding interaction, the binding kinetics, and the concentration of binding partners. In the context of affinity-controlled release--and specifically the discovery or design of binding partners--we review advances in in vitro selection and directed evolution of proteins, peptides, and oligonucleotides (aptamers), aided by computational design.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pakulska, Malgosia M -- Miersch, Shane -- Shoichet, Molly S -- Canadian Institutes of Health Research/Canada -- New York, N.Y. -- Science. 2016 Mar 18;351(6279):aac4750. doi: 10.1126/science.aac4750.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, and Donnelly Centre, University of Toronto, Toronto, Ontario, Canada. ; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada. ; Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, and Donnelly Centre, University of Toronto, Toronto, Ontario, Canada. Department of Chemistry, University of Toronto, Toronto, Ontario, Canada.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26989257" target="_blank"〉PubMed〈/a〉
    Keywords: Chemical Engineering ; Combinatorial Chemistry Techniques ; Delayed-Action Preparations/*chemistry ; Directed Molecular Evolution ; *Drug Design ; Humans ; Lactic Acid/*chemistry ; Microspheres ; Polyglycolic Acid/*chemistry ; Proteins/*administration & dosage
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 5
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    Antimicrobial activities of semi polar-nonpolar and polar secondary metabolites of sponge Dysidea pallescens from Hengam Island, Persian Gulf (2018)
    Details
    Publication Date: 2021-05-19
    Description: Sponges are the simplest multicellular animals that lack defense mechanisms and rely on chemical defense that have been used by mankind to develop antimicrobial drugs against diseases. The present study was designed to demonstrate the antibacterial and antifungal activities of marine sponge Dysidea pallescens semipolar and nonpolar extracts. In this study, D. pallescens were collected from Hengam Island in the Persian Gulf. The extracts were produced by Bligh and Dyer method. Broth Dilution Methods were used to check the antimicrobial activity of D. pallescens extracts against Escherichia coli (ATCC 15224), Pseudomonas aeruginosa (ATCC 25619), Staphylococcus aureus aureus (ATCC 1764), Bacillus subtilis pizizenii (ATCC 6633), Candida albicans (ATCC10231) and Aspergillus fumigates (PTCC5009). The results showed diethyl ether extract has bactericidal activity against S. aureus aureus (MBC=10mg/mL) and B. subtilis spizizenii (MBC=20mg/mL). D. pallescens diethyl ether extract showed a very weak antifungal activity but methanol extract showed fungicidal activity against A. fumigates (MFC=5mg/mL) and C. albicans (MFC=1.5 mg/mL). Therefore nonpolar-semipolar secondary metabolites of D. pallescens solutions in diethyl ether have shown significant antibacterial activity and polar- secondary metabolites solutions in methanol have shown significant antifungal activity.
    Description: Published
    Keywords: Biotechnology ; Dysidea pallescens ; Pseudomonas aeruginosa ; Escherichia coli ; Bacillus subtilis pizizenii ; Staphylococcus aureus aureus ; Candida albicans ; Aspergillus fumigates ; Antibacterial ; Antifungal ; Marine sponge
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.200-209
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  • 6
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    Isolation and screening of antibacterial and enzyme producing marine actinobacteria to approach probiotics against some pathogenic vibrios in shrimp Litopenaeus vannamei (2018)
    Details
    Publication Date: 2021-05-19
    Description: The application of new probiotics is a good strategy in the biological control of infectious diseases in aquaculture. Approximately 100 marine actinobacteria isolates were obtained from 10 sediment samples of shrimp farms. Heat treatment of sediment samples resulted in a selective reduction of the non actinobacterial heterotrophic microflora. Starch nitrate agar medium exhibited more efficacy than glycerol arginine agar medium for isolation. Twenty seven percent of actinobacterial isolates showed antagonistic activities against pathogenic Vibrio spp. All the antagonistic isolates showed the typical morphology of genus Streptomyces. Exoenzymatic activity screening showed that 44 %, 26%, 37% of antagonistic isolates represented amylase, lipase and protease activities, respectively. MNM-1400 strain exhibited highest antagonistic and exoenzymatic activity. The pathogenicity experiment revealed that MNM-1400 strain did not cause disease in Litopenaeus vannamei larvae. Extraction of produced antibacterial compounds by MNM-1400 strain showed that the active constituent didn’t have non polar property. Morphological, physiological and biochemical identification confirmed that MNM-1400 strain belonged to the genus streptomyces. Phylogenetic analysis by 16S rRNA gene sequencing showed a high similarity between MNM-1400 strain and Streptomyces californicus (similarity: 99%). These results suggest that the MNM-1400 strain can be considered as a potential probiotic in aquaculture
    Description: Published
    Keywords: Biotechnology ; Probiotic ; Marine actinobacteria ; Biological control ; Shrimp ; Litopenaeus vannamei ; Pathogenic
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.630-644
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  • 7
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    Antimicrobial and antioxidant effects of nisin Z and sodium benzoate in vacuum packed Caspian Kutum (Rutilus frisii) fillet stored at 4◦C (2018)
    Details
    Publication Date: 2021-05-19
    Description: This study was done to evaluate the antimicrobial and antioxidant effects of nisin Z (0.02 %) and sodium benzoate (1.5 and 2.5 %) in vacuum packed Caspian Kutum (Rutilus frisii) fillet stored at 4°C. Microbial changes [aerobic plate counts (APC), psychrotrophic counts (PTC) and lactic acid bacteria] and chemical indices [peroxide value and total volatile basic nitrogen (TVB-N)] were determined in days 0, 4, 8, 12 and 16. Results showed that PV and TVB-N in control samples (the sample dipped in prechilled distilled water) were deteriorated after 12 days compared to preserved samples which were acceptable after 16 days. Microbial tests indicated that control samples contained APC and PTC bacteria in day 16 more than standard limit, whereas treatment samples were in the acceptable range. In case of lactic acid bacteria, after 16 days, all samples were in the acceptable range. Results of chemical and microbial analysis showed that simultaneous use of nisin Z and sodium benzoate could increase the shelf life of vacuum packed R. frisii.
    Description: Published
    Keywords: Biotechnology ; Caspian Kutum ; Rutilus frisii ; Microbial quality ; Lipid oxidation ; Nisin Z ; Sodium benzoate ; Antimicrobial ; Temperature
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.789-801
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  • 8
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    Chitinase Isolated from Water and Soil Bacteria in Shrimp farming Ponds (2018)
    Details
    Publication Date: 2021-05-19
    Description: Chitinases have received attention because of their wide applications in the medicine, biotechnology, agriculture, waste management and industrial applications such as food quality enhancer and biopesticide. Excessive use of insecticides has led to several problems related to pollution and environmental degradation. In this study, isolation and identification of native bacterial strains with chitin hydrolysis activity, took place from water and soil of shrimp culture ponds in Bushehr and Abadan. To investigate the capacity of our chitinase for using in insecticide, biochemical properties of selected chitinase obtained in this research were compared to that of produced by Bacillus cereus p-1, isolated from an insecticide tablet. In this research, three mesophilic strains containing: Serratia marcescens B4A, Citrobacter freundii B1A and Bacillus cereus B3R were isolated. Results showed a 1600 bp band corresponding to chitinase gene. The similarity between temperature and pH profile and stability of chitinase was extracted from native bacteria and ones was obtained from Bacillus cereus p-1 implied that chitinase extracted from Serratia marcescens B4A has potential application in industry.
    Description: Published
    Keywords: Aquaculture ; Bacillus cereus ; Citrobacter freundii ; Serratia marcescens ; Food ; Chitinase ; Chitin ; Shrimp pond ; Bacteria ; Biotechnology
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.911-925
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  • 9
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    A putative probiotic isolated from hatchery reared juvenile Penaeus monodon (2018)
    Details
    Publication Date: 2021-05-19
    Description: Probiotic is considered as a remedy to solve disease problem in shrimp aquaculture. Shrimp body and environment bacterial flora are recommended as reliable source for extracting of beneficial bacteria. Therefore, 5000 pieces of post larvae of Penaeus monodon were distributed equally in 4 tanks in probiotic and antibiotic free condition up to juvenile. Then microbiological samples from digestive system, muscles, body surface, rearing water and tank sediment were inoculated onto the selective media plates. Incident of total bacteria, Vibrio, Pseudomonas and Entrobacteriaceae were evaluated in different body parts and rearing environment of shrimp. The highest bacteria count for total bacteria, Vibrio, Pseudomonas was occurred in sediment followed by digestive system. No Entrobacteriaceae was observed from all samples. Isolated bacteria were identified and placed in 7 genera consisted of Vibrio, Shewanella, Burkholderia, Clavibacter, Staphylococcus, Brevibacterium, Corynebacterium, while one genus remained unidentified. Genus Shewanella exhibited highest frequent bacteria in digestive system of P. monodon which may be indicated its ability to colonization in digestive system. Shewanella algae was selected from whole isolated bacteria as candidate probiotic via well plate diffusion technique and cross streak antagonistic test against Vibrio harveyi, V. parahaemolyticus and V. alginolyticus. It was able to digest lipid and protein in tributyrin agar and skim milk agar.
    Description: Published
    Keywords: Biotechnology ; Entrobacteriaceae ; Pseudomonas ; Vibrio ; Shewanella ; Burkholderia ; Clavibacter ; Staphylococcus ; Brevibacterium ; Corynebacterium ; Vibrio harveyi ; Probiotic ; Shewanella algae ; Penaeus monodon ; Shrimp ; Juvenile ; Hatchery
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.849-866
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  • 10
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    Effect of frozen storage on texture and color of fish burgers produced from Sarm surimi (2018)
    Details
    Publication Date: 2021-05-19
    Description: Surimi is a high quality myofibrillar protein concentrate that is obtained from cheap and underutilized fish species. In this research, surimi burger was prepared from Sarm (Scomberiodes commersonnianus) surimi (60%) and other ingredients. Some quality attributes of surimi burger were investigated during 3 months of frozen storage at -20°C. Fat value was determined in fresh raw surimi and surimi burger. Physical properties such as color stability (L, a and b values) and textural hardness before and after cooking were determined for surimi burgers during frozen storage at -20°C. Results showed that the hardness of surimi burgers and cooked samples were 768gf and 204gf, respectively at-the beginning of storage, and it was increased at the end of storage (921gf for surimi burger and 462gf for the cooked sample). Hardness showed significant difference through storage (P〈0.05). No major change was observed in the "L" value in surimi burger after 2 months; however it was decreased significantly after 3 months of frozen storage. In addition, no significant changes were observed in the "a" and "b" values in surimi burgers during frozen storage (P〉0.05).
    Description: Published
    Keywords: Scomberiodes commersonnianus ; Surimi ; Processing ; Quality ; Aquatics ; Biotechnology
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.161-166
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