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  • Articles  (32)
  • Growth  (16)
  • Health  (16)
  • Iranian Fisheries Science Research Institute  (31)
  • Jinja, Uganda  (1)
  • National Fisheries Resources Research Institute (NaFIRRI)  (1)
  • 2020-2022  (32)
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  • 1
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    Feasibility study on semiintensive polyculture of chainess and Indian carps in I.R.IRAN (Guilan province) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25352 | 18721 | 2018-09-14 06:57:48 | 25352 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: 15000 juvenile Indian majour carps (Labeo Rohita,Cirhinus merigala, Catla catla) imported (200 ) and transferred to the earthen ponds in Astaneh Fisheries Research Station , In order to assess the viability of rearing under the climatic condition of north part of Iran and also achieve some growth parameters from the larval stage to market size.The fry with 300 mg weight, were released in to 12 ponds. Specimens were separated to tree experimental categories: 1= (30%indian carp and 70% chinees carp) , 2= (50%indian carp and 50% chinees carp) , 3= (70%indian carp and 30% chinees carp) .single chinees carp production were used as control . fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured. Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings. Also the average weight of growing phase (second year of production ) was 647g for Labeo Rohita ,420 g. for Cirhinus merigala and 734g for Catla catla. The special growth rate (SGR)in Roho was 4.51 %.and 3.9 % for merigal and6.2 % for Catla while for Chinees carps SGR was 4.9, 6.7, 7.6 and 5.8 for silver carp,grass carp, common carp and big head respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility were remind for futhre studies.
    Keywords: Aquaculture ; Iran ; Guilan Province ; Astaneh ; Indian carp ; Growth ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Polyculture ; Juvenile ; Earthen ponds ; Fisheries ; Rearing ; Larval stage ; Fry ; Specimens ; Artificial food ; Fertilizers ; pH ; Turbidity ; Temperature
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  • 2
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    Study on viral nervouse necrosis (isolation, characterisation and pathogenesis) in golden grey mullet in the Caspian Sea and study of pathogenecity and possibility of transmission to the other fish species (Sturgeon fishes, Rutilus frisii kutum and reared Rainbow trout and Carp) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25435 | 18721 | 2018-09-23 16:47:09 | 25435 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Study on Viral Nervouse necrosis (isolation, characterisation and pathogenesis) in Golden grey mullet in the Caspian Sea and study of pathogenecity and possibility of transmission to the other fish species (Sturgeon fishes, Rutilus frisii kutum and reared Rainbow trout and Carp) Viral Nervous Necrosis (VNN) is a worldwide disease affecting several species of cultured marine fish. For the past two decades, betanodavirus infections that cause Viral Nervous Necrosis (VNN) have emerged as major constraints on the culture and sea ranching of marine fish in almost all parts of the world. More than forty species mainly of marine origin have been so far affected and this number is likely to rise in future following the introduction of new species and the increase of aquaculture trade. Unknown acute mortality occurred in wild golden grey mullet Lisa auratus and Liza saliens in Iranian waters of Caspian Sea in recent years. In order to isolation and confirmation of causative agents of golden grey mullet mortality in the Caspian Sea, a complementary research investigation project was designed in 2005 and approved immediately in Iranian Fisheries Research Organization (IFRO). Many diagnostic aspects such as Virology (Cell culture and Elctereone Microscopy), Hemathology, Bacteriology, Histopathology, Molecular biology (NestedRT-PCR), Heavy metals measuerment and Serology (IFAT and IHC) were employed in mentioned multidiciplinary project. About 322 moribund fish samples which revealed skin darkening, erratic swimming behavior such as spiral and belly-up at rest and high distention of swimming bladder. Suspected samples were collected from coastal capture sites in iranian north proviences in 2006 till 2009. Targets tissue such as brain and eye were removed in strile condition and then kept in -80oC frezzer for cell culture and Nested-RT-PCR. Other tissue samples from liver, kidney, intestine, stomach, gill, skin and muscle, gall bladder and gonads were taken and fixed in 10% buffer formalin and same parts fixed in glutaraldehyde 3% for histopathology, IHC and EM respectively. Cytopathic effect (CPE) was observed in those cell cultures just six days after inoculation with the dilutions of the tested 312 homogenate supernatants. CPE in monolayers of cells cultured (SSN-1 cell line) was characterised by thin or rounded, refractile, granular cells with vacuoles. Nine samples were positive in virology assay. Nested- RT-PCR was done on suspected tissue samples and supernatant of CPE positive samples and 21 tissue samples and all CPE positive samples were positive. IFAT was selected as a confirmatory method for identifying viral strains replicating on cell cultures and carried out with rabbit anti-betanodavirus serum on suspected tissue samples and some smears of CPE positive samples. Some bright points approved betanodavirus antigen and confirmed cell culture and Nested-RT-PCR findings. In fixed tissue samples widespread and massive vacuolation were observed in brain, spinal cord, retina and optical nerve. In order to confirmation of diagnostic findings , IHC was done with monoclonal antibody antibetanodavirus and some red-brown points were observed. Theses findings revealed expected viral antigens and confirmed previous results. Moreover, virus particles with 25-30 nm in diameter were visualized in infected brain and retina using positive staining in TEM. Also pathogenicity test was employed to confirm the obtained results. So Guppy fish Poecilia reticulata and sturgeon fry were used instead of the experimental host due to ease of handling and susceptibility. After 15 days post infection, guppy bathed in VNN-infected tissue culture with 104 TCID50 showed clinical signs similar to naturally infected Golden grey mullet, and the mortality rate reached up to 100% in 75 dpi. When target organs were examined by cell culture isolation, serology, and histopathology, all revealed the presence of virus in the Guppy. Suspected supernatant injected to sturgeon fry through intravitreous injection and widespread vacuolation were observed in brain and spinal cord buy IHC and Real time PCR were negative. In conclusion, with attntion to obtained results in this investigation such as ecological factors, clinical signs, histopathological, virological and bacteriological results, molecular analysis, (IHC, IFAT, PCR), TEM demonstration, serological and hematological findings, it could be confirmed that VNNV was the main causative agent for disease outbreak in Golden grey mullet in Southern coastline of Caspian Sea.
    Keywords: Aquaculture ; Health ; Iran ; Caspian Sea ; Viral nervous necrosis ; Golden grey mullet ; Liza aurata ; Liza saliens ; Histopathology ; Virology ; Bacteriology ; IHC ; IFAT ; PCR ; TEM ; Species ; Sturgeon ; Rutilus frisii kutum ; Rainbow trout ; Carp
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  • 3
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    Several fish species carp culture with Chinese black carp to increase diversity in warm water fish farms (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25447 | 18721 | 2018-09-25 18:12:04 | 25447 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The objective of this study was to investigate the effects of black carp introduction in chines carp poly culture as well as its effect on the growth, survival rate and production of chines carp species. This study conducted in two consecutive year. The period of culture in first year was during Jun 1 until Des 5 ,2009 and in second year it was from Jun 4 to Dec, 1,2010 .The experiment were started by selecting 9 earth end pond each with 800 m2 area and was performed in triplicate with 2 treatment and one control , each year .The ponds were treated by liming and fertilizer before filling water and then stocked with 3000 chines carp (140 Silvercarp, 42 Grass carp , 42 Common carp and 25 Bighead carp) while the number of Black carp in treatments 1, 2, 3 and 4, respectively, 250, 500, 75 and 125 pieces in Ha, blackcap was added to the control species respectively . The number of fish species harvested at end of culture in treatment 1, 2 and control .The total fish species harvested at the end of first year was 2276 out 1904 species released. The number of fishes catch in treatment 1,2 and control were 637,658and 573 species. the statistical analysis showed that average weight of black carp were 407±150 gr in treatment 1, 234±121 gr in treatment 2 with the minimum and maximum weight of 242 and 780 gr in first treatment as well as 110 and 750 gr in second treatment the production of black carp in first treatment were 42 kilogram per hectare and 60 kilogram in second treatment. The growth rate were higher in second treatment than first one. In second year the investigation repeated in triplicate with treatment3, 4 and one control. The total stocking density in treatment 3,4 and control were 665,633, and 574 pieces. Statistical analysis showed that total weight of black carp in third treatment was2049 ±614gr and in forth treatment was 2361 ±60 gr where the minimum and maximum values were 3115 gr and 1025 gr respectively. The survival rat of black carp in treatment 3 higher than in were treatment 4 while the growth in treatment 4 was higher treatment 3. The total production of black carp in treatment 3 and 4 were 123 and 181 kilogram per hectare respectively. The ANOVA test conducted for grass carp revealed that treatment 4 is significantly difference with treatment 3 and control. While for silver carp the treatment 4 is significantly difference with treatment 1 and control while for Big head carp the treatment 4 was significantly difference with control at 5% level (P〈%5). On second year the black carp reached to acceptable market size which could be compatible with other carp such as common carp and grass carp comparative analysis between different stocking density indicate that the stocking density with 125 pieces showed the highest positive effects.
    Keywords: Aquaculture ; Iran ; Fish ; Species ; Carp culture ; Chinese black carp ; Diversity ; Warm water ; Growth ; Survival rate ; Silver Carp ; Grass carp ; Common carp ; Bighead carp ; ANOVA
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  • 4
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    Investigation on probable viral agents associated with grass carp and silver carp mortality in Guilan province (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25812 | 18721 | 2018-10-13 09:36:14 | 25812 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Warm water aquaculture development along with import of eyed eggs, Broodstocks and ornamental fish also transferring of fingerlings and broodstocks from one province to another one has led to spreading of some unwanted diseases. According to the Gilan Fisheries Department report, mortality rate of silver carp(Hypophthalmichthys molitrix) in the summer of 2010 caused losing of silver carp production more than 1162 tons which was valued over than twenty-three milion rials that was increased to about 40 million rials in 1391. Also grass carp had a less mortality rate in some farms. Observed symptoms and severity of losses increased the probability of viral diseases such as spring viremia of carp, koi herpes virus and grass carp reovirus. In this research, totally 411 silver carp and grass carp with 5 to 2300 gram weight from 83 farms were sampled during 2011-2012. Clinical symptoms was different in various farms and subcutaneous hemorrhages on the flanks, red fin base or total fin and Red belly and operculum, mild exophthalmia, hemorrhaging in the abdominal cavity (yellow, transparent or red fluid) and petechial haemorrhages of skin and viscera and Ascites in abdominal cavity and some silver carps and grass carps were observed with palied liver and gill. In some cases swelling muscle was observed in Silver carp. Several fries with the severe emaciation were observed. Difference between clinical symptoms can indicate the presence of different causes of mortality in different farms. Various water colors from completely transparent to dark green, yellow or slightly brown observed in different pool, which shows the difference in overall culture management including water quality management. The minimum Water turbidity using secchi disk was 40 centimeters to one meter page in the fields. Source semi-deep wells or deep pools that were used as water source. Average water temperature, oxygen and pH in fileds 26/5-31 °C , 4/3-5/7 mg / L and 7/4-9/38. In order to virological surveys, sampling of internal organs, including kidneys, and spleen were done.and Homogeneous target tissues after spending 0/45 micron filter on EPC and BF2 cell line were inoculated to observe cell damage(CPE) in case of confirmation of antibody detection methods brilliant, RT-PCR and PCR done. In cell culture examination, a kind of cell damage (CPE-like changes) was observed after inoculating of 3 samples of Silver carp those were suspected to SVC, but in the second and third passages there were no sign of cell damage. It may be because of toxic effect of tissue filtrate on the cell monolayer. Concerning sample of Grass carp on two EPC and BF2 cells no evidence of cell damage was found. In indirect fluorescent antibody test, 72 hours after inoculation of tissues filtrates of of silver carp and grass carp samples on EPC, no positive reaction was observed. PCR and RT-PCR tests using specific primer pairs were done to test all of the silver carp and grass carp samples for SVC and KHV diseases Also for GCRV in grass carp samples. In addition, simultaneously commercial PCR kits applied for testing of SVC and KHV (IQ2000. Taiwan). The results of PCR and RT-PCR tests showed no evidence of robdovirus Carpio and grass carp reovirus RNA and no sign of koi herpesvirus DNA in tested samples. In conventional PCR and RT-PCR tests Using specific primer pairs none of positive bands related to SVCV(470 bp) and GCRV(292bp, 697bp and 320bp) were not confirmed in the tested samples. Also in electrophoresis of PCR products using IQ2000 kit revealed 471 bp and 640 bp negative bands in all samples and the positive bands were not observed. For confirmation of the results, three suspected samples were sent to Europe Union Reference Laboratory and none of mentioned diseases were confirmed. Simultaneously bacteriolocical examination applied for 26 using blood agar. In bacterial culture and related analyzes 6 isolates of Pseudomonas and 21isolates of Aeromonas hydrophila were detected from 26 farms while no virus was confirmed in the same samples. Based on the results of cell culture, PCR, RT-PCR and IFAT tests no one of SVCV, KHV and GCRV viruses were confirmed in tested samples. Therefore, the etiological reason of the mortalities in the tested farms was not viral diseases and should be prevent and control by biosecurity and health management in the farms.
    Keywords: Aquaculture ; Health ; Iran ; Gilan province ; SVCV ; KHV ; GCRV ; Silver Carp ; Grass carp ; Mortality
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  • 5
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    Epidemiological study on some environmental and management parameters affecting on WSD occurrence in Fenneropenaeus indicus and Penaeus vannamei (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25652 | 18721 | 2018-10-08 08:43:54 | 25652 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: For the first time white spot disease (WSD) was reported in shrimp farms of khoozestan province, in southwest of IRAN in 2002. Then in 2005 the neighbor province, boushehr, was contaminated. In 2008 WSD outbreak reported in sistan-bloochestan province in southeast of Iran. In 2015 all of southern shrimp farms of country except Hormozgan, the middle southern province, which has remained free of WSD, are being contaminated. White Spot disease suspended shrimp culture in thousands hectares of shrimp farms. Considering that white spot disease has not been observed in Hormozgan province yet, the question is; to what extent environmental and management factors participated in preventing WSD outbreak or cause WSD outbreak. In this study (20102012), the effects of environmental factors and management, stressors that decrease immune system function of shrimp are discussed. In addition, the role of pathogen as the main factor of outbreak is discussed. The goal of this study is to define environmental parameters and management practices associates with outbreak of white spot disease in affected provinces and discover reasons of being Hormozgan province free of this disease. In this study the role of the local environmental factors and management practice stressors in susceptibility to WSD was determine. Both the effects of environmental factors in water of ponds including total ammonia, nitrogen, dissolved oxygen, pH, salinity, transparency, and temperature and management issues related to biosecurity are studied. There were overlaps on physical and chemical parameter values obtained in clear areas with contaminated areas .Results of the data analysis suggest that lack of association with WSD incidence was 7 times greater than WSD incidence despite of disease outbreak in sistan-bloochestan province, so other sources of white spot disease virus incidence was suspected in affected areas. Histopathological examinations and polymerase chain reaction (PCR) tests during project performance did not reveal white spot disease virus evidences in post larvae examined from khoozestan province stocked in farms but disease outbreak was happened in that farms , so we suspected to management practice include feed , pond preparation and carrier of disease. Recorded values of temperature and salinity in some months during inspection in Hormozgan province specified stressful condition that may lead to WSD outbreak, however the disease did not appear. Therefore the hypothesis that the water physical and chemical conditions are reasons to prevent disease outbreak in Hormozgan province is being rejected. The policy of Hormozgan’s fishery authorities, to replaced Fenneropenaeus indicus with specific pathogen free Litopenaeus vannamei, that is more resistant to some of diseases, before incidence of WSD in farms and to before being endemic in the Hormozgan province, made an advantage compare to affected southern provinces that introduced Litopenaeus vannamei after WSD prevalence to their farms. However it does not guarantee to maintain current trend of being Hormozgan province farms free of white spot disease. Therefore establishing the principals of biosecurity are strongly emphasized. Strategies taken by the proficient authorities in preparation of SPF shrimp broodstock can be the most important factor in preventing WSD. Regarding biosecurity principals purchased feed must be free of shrimp head powder. Construction the new shrimp farms should be as far as it could be away from contaminated areas.
    Keywords: Aquaculture ; Ecology ; Health ; Iran ; Khouzestan Province ; Boushehr province ; Sistan and Balochestan province ; Hormozgan Province ; Epidemiological study ; WSD ; Fenneropenaeus indicus ; Penaeus vannamei ; Shrimp ; White Spot Disease ; Temperature ; Salinity ; PCR ; Polymerase Chain Reaction ; Ammonia ; Nitrogen ; Dissolved oxygen ; pH ; Transparency
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  • 6
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    Effect of salinity on spawning of yellow fin sea bream (Acanthopagrus latus) in spawning tanks (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25099 | 18721 | 2018-08-22 07:24:00 | 25099 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Salinity effects on brooders spawning, growth and survival rate of yellow fin sea bream (Acanthopagrus latus) fingerlings was studied in Khouzestan Marine Fishes Research Station (Bandar-e Imam) in 2002- 2003. More than 200 brooders were caught in Mahshar creeks using hook, and 6 males and 3 females of brooders were introduced to each 4 tons tank. The experiments were carried out using 3 salinity treatments (30±1, 35±1 and 40±1 ppt) in 3 replications. Survival rate of brooders in 4 tons tanks during late January to 4^th April was estimated more than %90 in all salinities and the maximum rate was observed in 30 ppt treatment. Spawned brooders percentage in 40 ppt was more than the two other salinities, and spawning occurred in all three replications of this treatment. In all treatment, spawning started from early march (late lunar month) at 19~'C and continued at 23°C. Duration and replication of spawnings in 40 ppt were more than other salinities, and last about 20 days. Released eggs (2461046), amount of eggs production per day (66413), average number of eggs per brooder (312914), and average eggs per kg body weight (649460) were found in 40 ppt more than other salinities. Floating eggs ratio was more than %90 in 40 ppt and it was significantly different to the others. Fertilization and hatching rates in 40 ppt treatment (86.7, 67 respectively) were more than 30 and 35 ppt but there was no significant difference. All spawnings cases , released eggs hatched.
    Keywords: Biology ; Iran ; Khouzestan Province ; Bandar-e-Imam ; Mahshar creeks ; Salinity ; Spawning ; Yellow fin ; Bream ; Acanthopagrus latus ; Propagation ; Density ; Survival rate ; Fingerling ; Growth ; Acanthopagrus latus ; Yellow fin sea bream
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  • 7
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    The survey on growth and survival larvae of different generation of Litopenaeus vannameiu (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25791 | 18721 | 2018-10-13 08:11:02 | 25791 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In the present Shrimp industry Has become a huge industry . The shrimp culture in Bushehr province began with Penaeus semisulcatus and Fenneropenaeus indicus and with introducing Litopenaeus vannamei in Iran Was known as a culture species. The shrimp production rate in IRAN is about 23000 metric ton. Outbreak of Various diseases and Biosecurity are most important things to produce Specific Patogen Free (SPF) shrimp. In this regard, production of SPF shrimp is The main requirements of shrimp industry. The survey on growth and srvival larve of different generation of Litopenaeus vannamei project was carried out in 2013 and 2014. Broodstock selecting, propagation, culture and shrimp generation Was performed in 2012 until 2014.In 2014 we produced 4300 pieces of prebroodstock with 22.38 gram and 86 percent survival rate after 120 days of culture.
    Keywords: Aquaculture ; Iran ; Bushehr province ; Litopenaeus vannmei ; Specific Patogen Free ; SPF ; Culture ; Shrimp culture ; Penaeus semisulcatus ; Fenneropenaeus indicus ; Survey ; Growth ; Survival ; Larvae
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  • 8
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    Issues of HIV/AIDS/ health among the fisher communities of Lake Albert and Kyoga (2021)
    National Fisheries Resources Research Institute (NaFIRRI) | Jinja, Uganda
    In:  http://aquaticcommons.org/id/eprint/20451 | 4230 | 2016-04-11 08:16:42 | 20451 | National Fisheries Resources Research Institute, Uganda
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    Publication Date: 2021-07-10
    Description: The fisheries resources of Lakes Albert and Kyoga present a high potential for economic growth, food, employment and foreign earnings. However, livelihoods appear to be compromised with the emergence and rapid spread of HIV/AIDS in the fisher communities of L. Albert and Kyoga. HIV/AIDS is considered a silent epidemic that is unique, posing a great challenge to the fisheries managers, health service providers, development planners and the resource users themselves. Fishers have high HIV prevalence, as well as AIDS-related illnesses and mortality rates. The high HIV prevalence rates among the fishing communities in Uganda is between 10-40% compared to the national rates which lie between 6% and 7%. This indicates that the national programmes have not adequately addressed the plight of the fishing communities of Lakes Albert, and Kyoga and the consequences have been devastating. Men and women living in fishing villages across the world have been found to be between five and ten times more vulnerable to the disease than other communities (Tarzan et al 2005, FAO, 2007). The present prevalence rates among the fishing communities stands at 10 to 40 % (LVFO, 2008). Meanwhile the same fishing communities are the essential labour for the Lakes’ fishery industry which is thriving nationally and internationally. That resource potentially can alleviate poverty and the HIV/AIDS threat. Fishing communities are the hidden victims of the disease, mixing patterns with the general population could act as a reservoir of infection that could spill over into the general population to drive the epidemic. On L. Albert, a quarter of the fisher folk were HIV-positive by 1992 compared to 4% in a nearby Agricultural village. Since then, there have been no targeted studies to address or monitor the prevalence rates eight years later, yet the multiplicity factor is high.HIV/AIDS can be linked to unsustainable fisheries, as the labour force available would not go to deep waters to fish, instead would fish in the shallow waters as a coping mechanism. A further effect is the loss to National and local economies and reduced nutritional security for the wider population. HIV/AIDS remains a significant challenge that has created a mosaic of complexity in the fishery sector. This needs to be addressed. It is, therefore, paramount that a comprehensive study was under taken to address this pandemic and the phenomenon of HIV/AIDS based on the study objectives.1. To determine the trend in HIV/AIDS infection among fishing communities and the factors affecting it2. To assess the impacts of HIV/AIDS on fish production and the implications for fisheries management.
    Keywords: Fisheries ; Health
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  • 9
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    Rearing of Indian craps fries to 1g weight in earthen ponds (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25351 | 18721 | 2018-09-13 12:58:15 | 25351 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: 30000 juvenile Indian majour carps (Labeo Rohita,Cirhinus merigala, Catla catla) imported (2007 and 2008) and transferred to the earthen ponds in Astaneh Fisheries Research Station (Gilan) and Sheiban Resaerch center (Khozestan), In order to assess the viability of rearing fry up to 1 gram fingerlings under the climatic condition of north and south part of Iran . Growth parameters from the larval stage to 1 g were studied also. The fry with 300 mg weight, were released in to 3ponds.specimens were separated to tree experimental categories. fishes were fed with artificial food and also with the natural products of the ponds which were enriched with organic fertilizers, after being equally stocked in the ponds .During the experiment, oxygen level ,PH, turbidity, and temperature were measured . Monthly fry samples were taken, for weight and length biometrical analysis and also determination of the weight gain percentage, average daily growth rate, specific growth rate. Results have indicated adaptations and considerable growth in this species, with an increase from the initial weight of 300 mg to an average of 13.5±1.6 mg in 50 days period until the end of November for fingerlings.Catla was gain 1 g. during 15 day while Roho and Merigal were gain 1 g. during 14 and 10 days respectively. Results revealed that the Indian majour carps compatibility with the condition in Gilan province was succesfull and the combination of species and optimization of their compatibility specialy in other provinces like Sistan and Baluchestan were remind for futhre studies.
    Keywords: Aquaculture ; Iran ; Guilan Province ; Astaneh ; Indian carp ; Growth ; Labeo Rohita ; Cirhinus merigala ; Catla catla ; Juvenile ; Earthen ponds ; Fisheries ; Fry ; Fingerlings ; Artificial food ; Oxygen level ; Turbidity ; Fertilizers
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  • 10
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    The study on Zataria multiflora essence application in control of fungal contamination of some cultured species of aquatic animals (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25364 | 18721 | 2018-09-14 07:14:44 | 25364 | Iranian Fisheries Science Research Institute
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    Publication Date: 2021-07-16
    Description: In order to do this study, 150 Persian sturgeon having 75g mean weight transferred to tanks with 500L volume. After dividing the fishes into two groups (experimental and control group), the fingerlings fed by concentrate food that contained different levels of Zataria multiflora essential oil (15g/kg, 25g/kg, 50g/kg, 100g/kg) for 8 weeks. Finally, sampling carried out on blood and serum of fishes. After preparing the samples based on standard methods, haematology and serology studies, performed. The results showed significant difference in mean and standard deviation of monocytes between experimental and control groups (P〈0.05). In treatment 25g/kg, monocyte count was more than other treatments. But there are no significant differences in mean and standard deviation of other white blood cells. The results of serologic studies showed no significant differences in serum indices (C3, C4 and total protein) (P〈0.05), but there is significant difference in mean and standard deviation of albumin. So that , the mean and standard deviation in treatments 15g/kg and 25g/kg and control group were more than treatments 50g/kg and 100g/kg of Zataria multiflora essential oil. Also , this is the first record of the anesthetic effect of Zataria multiflora essence on fish. In this research the effect of 50 and 100 ppm concentrations of Zataria multiflora essence on the rainbow trout (Oncorhynchus mykiss) fry with average weight of 15±2 grams and Salmo trutta caspius with average weight of 40±4 grams has been studied. In this research the water temperature was 16-17 ºC, dissolved oxygen 7±0.2 and pH═8. The average time of coming to unconscious in 50 ppm and 100 ppm concentration in both Oncorhyncus mykiss and Salmo trutta caspius was 3 and 2 minutes respectively; and the means of balance and return of anesthesia were 2 and 3 minutes respectively, and the average time of inducing balance and recovering consciousness was 2 and 3 minutes respectively. Moreover, in contrast there is a significant difference between the time of inducing unconsciousness and recovery in all kinds of fish (p 〈 0.0001). The fish anesthetized with 100 ppm concentration after transference to water without Zataria multiflora essence were calm for 30 minutes and showed no reaction to weak stimuli (such as touching). In regard of the time of falling into unconsciousness and the time of recovery by different degrees of concentration of Zataria multiflora essence there is no significant difference between Oncorhyncus mykiss and Salmo trutta caspius (p 〉 0.05). This research also shows that Zataria multiflora essence has an anesthetic effect but because of its unwanted severe irreparable breathing (respiration) side effects and also the deformation of the fish which is against the fisheries’ ethics (death accompanied with extreme pain) it would be better not to be used as anesthetics. One of the advantages of Zataria multiflora essence as anesthetics is its low concentration (25 ppm) for anesthetizing the fish and also its economical cost. In addition, this essence has antimicrobial and disinfecting effects and, therefore, it can remove the infection from the external surface of the body of the fish. One of the most noticeable defects of Zataria multiflora essence is that at the time of anesthetizing it causes a state of excitement and irritability in the fish which manifests itself in the speed of swimming and the possibility of jumping out of water. As a result, although Zataria multiflora essence has anesthetizing effects, it is recommended not to be used for anaesthetizing the fish, because it posses unwanted side effects that may even cause the death of the fish; however, it may be used as a sedative during manipulating the fish.
    Keywords: Aquaculture ; Health ; Iran ; Zataria multiflora ; Essential oils ; Persian Sturgeon ; Trout ; Zataria multiflora
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 222
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