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  • Mice  (2)
  • Binding Sites  (1)
  • Superfluidity and superconductivity
  • American Association for the Advancement of Science (AAAS)  (3)
  • 2010-2014  (3)
  • 2013  (3)
  • 1
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    Probing allostery through DNA (2013)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2013-02-16
    Beschreibung: Allostery is well documented for proteins but less recognized for DNA-protein interactions. Here, we report that specific binding of a protein on DNA is substantially stabilized or destabilized by another protein bound nearby. The ternary complex's free energy oscillates as a function of the separation between the two proteins with a periodicity of ~10 base pairs, the helical pitch of B-form DNA, and a decay length of ~15 base pairs. The binding affinity of a protein near a DNA hairpin is similarly dependent on their separation, which-together with molecular dynamics simulations-suggests that deformation of the double-helical structure is the origin of DNA allostery. The physiological relevance of this phenomenon is illustrated by its effect on gene expression in live bacteria and on a transcription factor's affinity near nucleosomes.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586787/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586787/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kim, Sangjin -- Brostromer, Erik -- Xing, Dong -- Jin, Jianshi -- Chong, Shasha -- Ge, Hao -- Wang, Siyuan -- Gu, Chan -- Yang, Lijiang -- Gao, Yi Qin -- Su, Xiao-dong -- Sun, Yujie -- Xie, X Sunney -- DP1 OD000277/OD/NIH HHS/ -- New York, N.Y. -- Science. 2013 Feb 15;339(6121):816-9. doi: 10.1126/science.1229223.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23413354" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Allosteric Regulation ; Base Sequence ; Binding Sites ; DNA, B-Form/*chemistry ; DNA-Binding Proteins/*chemistry ; DNA-Directed RNA Polymerases/chemistry ; Escherichia coli/genetics/metabolism ; Gene Expression ; *Gene Expression Regulation, Bacterial ; Lac Repressors/chemistry ; Molecular Dynamics Simulation ; Nucleosomes/chemistry ; Protein Binding ; Protein Structure, Tertiary ; Receptors, Glucocorticoid/chemistry ; Transcription Factors/*chemistry ; Viral Proteins/chemistry
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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    AKTUELLE ARTIKEL
  • 2
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    CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins (2013)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2013-12-21
    Beschreibung: The duration of a woman's reproductive period is determined by the size and persistence of a dormant oocyte pool. Specific oocyte genes are essential for follicle maintenance and female fertility. The mechanisms that regulate the expression of these genes are poorly understood. We found that a cullin-ring finger ligase-4 (CRL4) complex was crucial in this process. Oocyte-specific deletion of the CRL4 linker protein DDB1 or its substrate adaptor VPRBP (also known as DCAF1) caused rapid oocyte loss, premature ovarian insufficiency, and silencing of fertility maintaining genes. CRL4(VPRBP) activates the TET methylcytosine dioxygenases, which are involved in female germ cell development and zygote genome reprogramming. Hence, CRL4(VPRBP) ubiquitin ligase is a guardian of female reproductive life in germ cells and a maternal reprogramming factor after fertilization.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yu, Chao -- Zhang, Yin-Li -- Pan, Wei-Wei -- Li, Xiao-Meng -- Wang, Zhong-Wei -- Ge, Zhao-Jia -- Zhou, Jian-Jie -- Cang, Yong -- Tong, Chao -- Sun, Qing-Yuan -- Fan, Heng-Yu -- New York, N.Y. -- Science. 2013 Dec 20;342(6165):1518-21. doi: 10.1126/science.1244587.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Life Sciences Institute and Innovation Center for Cell Biology, Zhejiang University, Hangzhou 310058, China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24357321" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Carrier Proteins/genetics/*metabolism ; Cell Survival/genetics/physiology ; Cellular Reprogramming/*genetics ; Cullin Proteins/genetics/metabolism ; DNA-Binding Proteins/genetics/*metabolism ; Dioxygenases/genetics/*metabolism ; Female ; Fertility/*genetics ; Gene Silencing ; Gonadal Dysgenesis/genetics ; HeLa Cells ; Humans ; Mice ; Mice, Knockout ; Oocytes/*physiology ; Ovary/physiopathology ; Proto-Oncogene Proteins/genetics/*metabolism
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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    AKTUELLE ARTIKEL
  • 3
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    Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds (2013)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 2013-07-23
    Beschreibung: Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinical applications. Here, we show that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds. The chemically induced pluripotent stem cells resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. By using small molecules, exogenous "master genes" are dispensable for cell fate reprogramming. This chemical reprogramming strategy has potential use in generating functional desirable cell types for clinical applications.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hou, Pingping -- Li, Yanqin -- Zhang, Xu -- Liu, Chun -- Guan, Jingyang -- Li, Honggang -- Zhao, Ting -- Ye, Junqing -- Yang, Weifeng -- Liu, Kang -- Ge, Jian -- Xu, Jun -- Zhang, Qiang -- Zhao, Yang -- Deng, Hongkui -- New York, N.Y. -- Science. 2013 Aug 9;341(6146):651-4. doi: 10.1126/science.1239278. Epub 2013 Jul 18.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉College of Life Sciences and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23868920" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Cadherins/genetics ; Cell Engineering/*methods ; Cellular Reprogramming/*drug effects/genetics ; Epithelial-Mesenchymal Transition/drug effects/genetics ; Fibroblasts/cytology/*drug effects ; Gene Expression Profiling ; Green Fluorescent Proteins/genetics ; Induced Pluripotent Stem Cells/*cytology/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Octamer Transcription Factor-3/genetics/metabolism ; Promoter Regions, Genetic/drug effects ; Small Molecule Libraries/chemistry/*pharmacology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    AKTUELLE ARTIKEL
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