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  • *Solar System  (7)
  • Cell Line  (6)
  • 1995-1999  (13)
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  • 1995  (13)
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  • 1995-1999  (13)
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  • 1995  (13)
  • 1
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    The RNA component of human telomerase (1995)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1995-09-01
    Beschreibung: Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA)-protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5'-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG)n. Germline tissues and tumor cell lines expressed more hTR than normal somatic cells and tissues, which have no detectable telomerase activity. Human cell lines that expressed hTR mutated in the template region generated the predicted mutant telomerase activity. HeLa cells transfected with an antisense hTR lost telomeric DNA and began to die after 23 to 26 doublings. Thus, human telomerase is a critical enzyme for the long-term proliferation of immortal tumor cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Feng, J -- Funk, W D -- Wang, S S -- Weinrich, S L -- Avilion, A A -- Chiu, C P -- Adams, R R -- Chang, E -- Allsopp, R C -- Yu, J -- AG09383/AG/NIA NIH HHS/ -- New York, N.Y. -- Science. 1995 Sep 1;269(5228):1236-41.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Geron Corporation, Menlo Park, CA 94025, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7544491" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Base Sequence ; Cell Death ; *Cell Division ; Cell Line ; Cloning, Molecular ; DNA Nucleotidylexotransferase/antagonists & ; inhibitors/chemistry/genetics/*metabolism ; HeLa Cells ; Humans ; Molecular Sequence Data ; Oligonucleotides, Antisense/pharmacology ; Polymerase Chain Reaction ; RNA/chemistry/genetics/*metabolism ; Templates, Genetic ; Transfection ; Tumor Cells, Cultured
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 2
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    A region of adenylyl cyclase 2 critical for regulation by G protein beta gamma subunits (1995)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1995-05-26
    Beschreibung: Receptor-mediated activation of heterotrimeric guanine nucleotide-binding proteins (G proteins) results in the dissociation of alpha from beta gamma subunits, thereby allowing both to regulate effectors. Little is known about the regions of effectors required for recognition of G beta gamma. A peptide encoding residues 956 to 982 of adenylyl cyclase 2 specifically blocked G beta gamma stimulation of adenylyl cyclase 2, phospholipase C-beta 3, potassium channels, and beta-adrenergic receptor kinase as well as inhibition of calmodulin-stimulated adenylyl cyclases, but had no effect on interactions between G beta gamma and G alpha o. Substitutions in this peptide identified a functionally important motif, Gln-X-X-Glu-Arg, that is also conserved in regions of potassium channels and beta-adrenergic receptor kinases that participate in G beta gamma interactions. Thus, the region defined by residues 956 to 982 of adenylyl cyclase 2 may contain determinants important for receiving signals from G beta gamma.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, J -- DeVivo, M -- Dingus, J -- Harry, A -- Li, J -- Sui, J -- Carty, D J -- Blank, J L -- Exton, J H -- Stoffel, R H -- CA-44998/CA/NCI NIH HHS/ -- DK-37219/DK/NIDDK NIH HHS/ -- DK-38761/DK/NIDDK NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1995 May 26;268(5214):1166-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, Mount Sinai School of Medicine, City University of New York, NY 10029, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7761832" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Adenylyl Cyclase Inhibitors ; Adenylyl Cyclases/*chemistry/metabolism ; Amino Acid Sequence ; Animals ; Cell Line ; Enzyme Activation/physiology ; GTP-Binding Proteins/chemistry/*physiology ; Guanosine Triphosphate/physiology ; In Vitro Techniques ; Molecular Sequence Data ; Peptide Fragments/chemical synthesis/chemistry/physiology ; Potassium Channels/physiology ; Rats ; Receptor Protein-Tyrosine Kinases/metabolism ; Receptors, Adrenergic, beta/metabolism ; Signal Transduction/physiology ; Structure-Activity Relationship ; Type C Phospholipases/metabolism
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
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    The Hubble Space Telescope (HST) observing campaign on comet Shoemaker-Levy 9 (1995)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1995-03-03
    Beschreibung: The Hubble Space Telescope made systematic observations of the split comet P/Shoemaker-Levy 9 (SL9) (P designates a periodic comet) starting in July 1993 and continuing through mid-July 1994 when the fragments plunged into Jupiter's atmosphere. Deconvolutions of Wide Field Planetary Camera images indicate that the diameters of some fragments may have been as large as approximately 2 to 4 kilometers, assuming a geometric albedo of 4 percent, but significantly smaller values (that is, 〈 1 kilometer) cannot be ruled out. Most of the fragments (or nuclei) were embedded in circularly symmetric inner comae from July 1993 until late June 1994, implying that there was continuous, but weak, cometary activity. At least a few nuclei fragmented into separate, condensed objects well after the breakup of the SL9 parent body, which argues against the hypothesis that the SL9 fragments were swarms of debris with no dominant, central bodies. Spectroscopic observations taken on 14 July 1994 showed an outburst in magnesium ion emission that was followed closely by a threefold increase in continuum emission, which may have been caused by the electrostatic charging and subsequent explosion of dust as the comet passed from interplanetary space into the jovian magnetosphere. No OH emission was detected, but the derived upper limit on the H2O production rate of approximately 10(27) molecules per second does not necessarily imply that the object was water-poor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Weaver, H A -- A'Hearn, M F -- Arpigny, C -- Boice, D C -- Feldman, P D -- Larson, S M -- Lamy, P -- Levy, D H -- Marsden, B G -- Meech, K J -- New York, N.Y. -- Science. 1995 Mar 3;267(5202):1282-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Space Telescope Science Institute, Baltimore, MD 21218.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7871424" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Atmosphere ; *Extraterrestrial Environment ; Hydroxyl Radical/analysis ; *Jupiter ; Magnesium/analysis ; *Solar System ; Spectrum Analysis ; Water/analysis
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
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    Inactivation of the mouse Huntington's disease gene homolog Hdh (1995)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1995-07-21
    Beschreibung: Huntington's disease (HD) is a dominant neurodegenerative disorder caused by expansion of a CAG repeat in the gene encoding huntingtin, a protein of unknown function. To distinguish between "loss of function" and "gain of function" models of HD, the murine HD homolog Hdh was inactivated by gene targeting. Mice heterozygous for Hdh inactivation were phenotypically normal, whereas homozygosity resulted in embryonic death. Homozygotes displayed abnormal gastrulation at embryonic day 7.5 and were resorbing by day 8.5. Thus, huntingtin is critical early in embryonic development, before the emergence of the nervous system. That Hdh inactivation does not mimic adult HD neuropathology suggests that the human disease involves a gain of function.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Duyao, M P -- Auerbach, A B -- Ryan, A -- Persichetti, F -- Barnes, G T -- McNeil, S M -- Ge, P -- Vonsattel, J P -- Gusella, J F -- Joyner, A L -- NS16367/NS/NINDS NIH HHS/ -- NS32765/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1995 Jul 21;269(5222):407-10.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown 02129, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7618107" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Animals ; Base Sequence ; Cell Line ; Ectoderm/cytology ; Embryonic and Fetal Development ; Female ; Gene Targeting ; Genotype ; Heterozygote ; Homozygote ; Humans ; Huntington Disease/*genetics ; Male ; Mesoderm/cytology ; Mice ; Mice, Inbred C57BL ; Molecular Sequence Data ; Nerve Tissue Proteins/*genetics/physiology ; Nuclear Proteins/*genetics/physiology ; Phenotype ; Stem Cells/metabolism
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
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    HST imaging of atmospheric phenomena created by the impact of comet Shoemaker-Levy 9 (1995)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1995-03-03
    Beschreibung: Hubble Space Telescope (HST) images reveal major atmospheric changes created by the collision of comet Shoemaker-Levy 9 with Jupiter. Plumes rose to 3000 kilometers with ejection velocities on the order of 10 kilometers second-1; some plumes were visible in the shadow of Jupiter before rising into sunlight. During some impacts, the incoming bolide may have been detected. Impact times were on average about 8 minutes later than predicted. Atmospheric waves were seen with a wave front speed of 454 +/- 20 meters second-1. The HST images reveal impact site evolution and record the overall change in Jupiter's appearance as a result of the bombardment.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hammel, H B -- Beebe, R F -- Ingersoll, A P -- Orton, G S -- Mills, J R -- Simon, A A -- Chodas, P -- Clarke, J T -- De Jong, E -- Dowling, T E -- New York, N.Y. -- Science. 1995 Mar 3;267(5202):1288-96.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, Cambridge 02139.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7871425" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Atmosphere ; *Extraterrestrial Environment ; *Jupiter ; *Solar System
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 6
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    HST far-ultraviolet imaging of Jupiter during the impacts of comet Shoemaker-Levy 9 (1995)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1995-03-03
    Beschreibung: Hubble Space Telescope far-ultraviolet images of Jupiter during the Shoemaker-Levy 9 impacts show the impact regions darkening over the 2 to 3 hours after the impact, becoming darker and more extended than at longer wavelengths, which indicates that ultraviolet-absorbing gases or aerosols are more extended, more absorbing, and at higher altitudes than the absorbers of visible light. Transient auroral emissions were observed near the magnetic conjugate point of the K impact site just after that impact. The global auroral activity was fainter than average during the impacts, and a variable auroral emission feature was observed inside the southern auroral oval preceding the impacts of fragments Q1 and Q2.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Clarke, J T -- Prange, R -- Ballester, G E -- Trauger, J -- Evans, R -- Rego, D -- Stapelfeldt, K -- Ip, W -- Gerard, J C -- Hammel, H -- New York, N.Y. -- Science. 1995 Mar 3;267(5202):1302-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Space Physics Research Laboratory, University of Michigan, Ann Arbor 48109.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7871427" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Atmosphere ; *Extraterrestrial Environment ; *Jupiter ; *Solar System
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 7
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    The southern high-speed stream: results from the SWICS instrument on Ulysses (1995)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1995-05-19
    Beschreibung: The high-speed solar wind streaming from the southern coronal hole was remarkably uniform and steady and was confined by a sharp boundary that extended to the corona and chromosphere. Charge state measurements indicate that the electron temperature in this coronal hole reached a maximum of about 1.5 million kelvin within 3 solar radii of the sun. This result, combined with the observed lack of depletion of heavy elements, suggests that an additional source of momentum is required to accelerate the polar wind.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Geiss, J -- Gloeckler, G -- von Steiger, R -- Balsiger, H -- Fisk, L A -- Galvin, A B -- Ipavich, F M -- Livi, S -- McKenzie, J F -- Ogilvie, K W -- New York, N.Y. -- Science. 1995 May 19;268(5213):1033-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Physikalisches Institut, University of Bern, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7754380" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Elements ; *Extraterrestrial Environment ; Ions ; *Solar System ; Spacecraft ; Spectrum Analysis/instrumentation
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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  • 8
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    Collision of comet Shoemaker-Levy 9 with Jupiter observed by the NASA infrared telescope facility (1995)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1995-03-03
    Beschreibung: The National Aeronautics and Space Administration (NASA) Infrared Telescope Facility was used to investigate the collision of comet Shoemaker-Levy 9 with Jupiter from 12 July to 7 August 1994. Strong thermal infrared emission lasting several minutes was observed after the impacts of fragments C, G, and R. All impacts warmed the stratosphere and some the troposphere up to several degrees. The abundance of stratospheric ammonia increased by more than 50 times. Impact-related particles extended up to a level where the atmospheric pressure measured several millibars. The north polar near-infrared aurora brightened by nearly a factor of 5 a week after the impacts.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Orton, G -- A'Hearn, M -- Baines, K -- Deming, D -- Dowling, T -- Goguen, J -- Griffith, C -- Hammel, H -- Hoffmann, W -- Hunten, D -- New York, N.Y. -- Science. 1995 Mar 3;267(5202):1277-82.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Jet Propulsion Laboratory, California Institute of Technology, Pasadena 91109.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7871423" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Ammonia/analysis ; Atmosphere ; Carbon Monoxide/analysis ; *Extraterrestrial Environment ; *Jupiter ; *Solar System ; Temperature ; United States ; United States National Aeronautics and Space Administration
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
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    Response of the Io plasma torus to comet Shoemaker-Levy 9 (1995)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1995-03-03
    Beschreibung: Spectroscopic and imaging observations of the Io plasma torus were made in June and July 1994 in conjunction with the encounter of periodic comet Shoemaker-Levy 9 with Jupiter. Characteristic emissions from sulfur and oxygen ions showed a decline of about 30 percent in the extreme ultraviolet and an increase of about 40 percent in the far ultraviolet relative to preimpact observations. Changes in the extreme ultraviolet may be indicative of small changes in the torus electron temperature as a result of quenching of electrons by dust associated with the comet passage. However, no new emission features indicative of fragment dust within the torus were detected. The characteristic torus morphology seen in ground-based imaging was typical of that observed in the past.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McGrath, M A -- Hall, D T -- Matheson, P L -- Weaver, H A -- Trauger, J T -- Smith, T E -- Thomas, N -- Gladstone, R -- Schneider, N M -- Harris, W M -- New York, N.Y. -- Science. 1995 Mar 3;267(5202):1313-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Space Telescope Science Institute, Baltimore, MD 21218.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7871429" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Atmosphere ; *Extraterrestrial Environment ; *Jupiter ; Magnetics ; *Solar System ; Sulfur/analysis ; Temperature
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
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    CD1-restricted T cell recognition of microbial lipoglycan antigens (1995)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1995-07-14
    Beschreibung: It has long been the paradigm that T cells recognize peptide antigens presented by major histocompatibility complex (MHC) molecules. However, nonpeptide antigens can be presented to T cells by human CD1b molecules, which are not encoded by the MHC. A major class of microbial antigens associated with pathogenicity are lipoglycans. It is shown here that human CD1b presents the defined mycobacterial lipoglycan lipoarabinomannan (LAM) to alpha beta T cell receptor-bearing lymphocytes. Presentation of these lipoglycan antigens required internalization and endosomal acidification. The T cell recognition required mannosides with alpha(1--〉2) linkages and a phosphotidylinositol unit. T cells activated by LAM produced interferon gamma and were cytolytic. Thus, an important class of microbial molecules, the lipoglycans, is a part of the universe of foreign antigens recognized by human T cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sieling, P A -- Chatterjee, D -- Porcelli, S A -- Prigozy, T I -- Mazzaccaro, R J -- Soriano, T -- Bloom, B R -- Brenner, M B -- Kronenberg, M -- Brennan, P J -- AI 07118/AI/NIAID NIH HHS/ -- AI 18357/AI/NIAID NIH HHS/ -- AI 28973/AI/NIAID NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1995 Jul 14;269(5221):227-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Dermatology, University of California at Los Angeles (UCLA) School of Medicine 90095, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7542404" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): *Antigen Presentation ; Antigen-Presenting Cells/immunology ; Antigens, CD/*immunology ; Antigens, CD1 ; Carbohydrate Conformation ; Carbohydrate Sequence ; Cell Line ; Humans ; Interferon-gamma/secretion ; Interleukin-4/secretion ; Leprosy/*immunology ; Lipopolysaccharides/*immunology ; Lymphocyte Activation ; Molecular Sequence Data ; Mycobacterium leprae/immunology ; Phosphatidylinositols/immunology ; Receptors, Antigen, T-Cell, alpha-beta/immunology ; Species Specificity ; T-Lymphocytes, Cytotoxic/*immunology
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
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