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  • Lepidoptera  (14)
  • PCR  (9)
  • Scolytidae
  • Springer  (24)
  • 2005-2009
  • 1990-1994  (24)
  • 1980-1984
  • 1965-1969
  • 1993  (24)
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Keywords
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  • Springer  (24)
Years
  • 2005-2009
  • 1990-1994  (24)
  • 1980-1984
  • 1965-1969
Year
  • 1
    Electronic Resource
    Electronic Resource
    Geometrical and optical isomerism of pheromones in two sympatricDryocoetes species (Coleoptera: Scolytidae), mediates species specificity and response level (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 2169-2182 
    Details
    ISSN: 1573-1561
    Keywords: Semiochemicals ; pheromones ; Dryocoetes confusus ; Dryocoetes affaber ; Coleoptera ; Scolytidae ; enantiomers ; diastereoisomers ; exo-brevicomin ; endo-brevicomin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In a field-trapping experiment, western balsam bark beetles,Dryocoetes confusus Swaine, were highly attracted to a 5∶1 mixture of (±)-exo-and (±)-endo-brevicomin. Beetles in the sympatric speciesD. affaber (Mann.), were best attracted to a 1∶1 blend of these semiochemicals [either (±)∶(±) or (±)∶(±)], suggesting that both geometrical isomers are pheromone components in these species. In laboratory bioassays and further field experiments, attraction ofD. confusus was greatest when the (+) enantiomers of both geometrical isomers of brevicomin were presented in a 9∶1 ratio. Responses by maleD. confusus to attractive mixtures were reduced in the presence of (−)-exo-brevicomin. Exploitation of the complete range of variability in pheromone structure (both geometrical and optical isomerism) would allow for optimization and regulation of response levels within a species and also could maintain reproductive isolation among sympatric congeneric species primarily through production and response to species-specific blends.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00979655
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  • 2
    Electronic Resource
    Electronic Resource
    Constituents of wing gland and abdominal hair pencil secretions of male African sugarcane borer,Eldana saccharina walker (Lepidoptera: Pyralidae) (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 2255-2277 
    Details
    ISSN: 1573-1561
    Keywords: Eldana saccharina ; Lepidoptera ; Pyralidae ; exocrine secretions ; sex pheromone ; aggregation pheromone ; electroantennograms ; electroantennographic detection ; NMR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In addition totrans-3,7-dimethyl-6-octen-4-olide (eldanolide), vanillin, and 4-hydroxybenzaldehyde, identified by French workers in the wing gland and abdominal hair pencil secretions of the male African sugarcane borer,Eldana saccharina, we have, in an earlier note, reported the presence of several other terpenoid, aromatic, and unbranched-chain compounds such as, (Z)-3,7-dimethylocta-2,6-dienoic acid, 6,10,14-trimethyl-2-pentadecanol, 4-hydroxy-3-methoxybenzyl alcohol, 1-octadecane thiol, 16-hexadecanolide, and 18-octadecanolide in these secretions. In the present paper experimental details and spectral evidence supporting the identification of these compounds, as well as the identification of (Z)-9-hexadecenal and cw-3,7-di-methyl-6-octen-4-olide (cis-eldanolide), are reported. Using electroantennography it was found that male and female antennae reacted approximately equally strongly to both secretions. This result was confirmed in analyses of the secretions using coupled gas chromatography-electroantennography and it was found that male as well as female antennae responded to eldanolide. Vanillin, substituted phenols related to vanillin, and some oxygenated monoterpenes elicited weak responses in male and female antennae. In some analyses 6,10,14-trimethyl-2-pentadecanol, present in the secretions of the insect, gave a strong antennal response. The results obtained in dynamic and static headspace determinations showed that several of the organic compounds present in the glandular secretions are released in detectable quantities and are present in widely varying quantitative ratios in the effluvia of individual calling male moths.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00979662
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  • 3
    Electronic Resource
    Electronic Resource
    Sugar beet crown borer,Hulstia undulatella (Clemens) 1: Identification and field tests of female sex pheromone gland components (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 433-440 
    Details
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Pyralidae ; Phycitinae ; Hulstia undulatella ; sex pheromone ; (Z)-9-tetradecen-1-ol acetate ; (Z)-9-tetradecen-1-ol ; (Z)-11-hexa-decen-1-ol acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electroantennogram profiles of saturated and monounsaturated 12-, 14-, and 16-carbon acetates, and 12- and 14-carbon alcohols implicated (Z)-9-tetradecen-1-ol acetate (Z9-14: Ac) as a component of the female sex pheromone ofHulstia undulatella (Clemens). Gas chromatography-mass spectrometric analysis of extract of the female sex pheromone glands showed the presence of Z9-14:Ac (8.5 ng/female), (Z)-9-tetradecen-1-ol (Z9-14:OH), and (Z)-11-hexadecen-1-ol acetate (Z11-16:Ac) in a ratio of 100∶4∶21, respectively. In tests in sugar beet fields, Z9-14:Ac alone produced some trap catch. Addition of Z9-14: OH did not increase catch while addition of Z11-16:Ac eliminated catch, but addition of both Z9-14:OH and Z11-16: Ac increased catch sevenfold. A combination of Z9-14: OH and Z11-16: Ac without Z9-14: Ac did not produce trap catch. A lure of 200 μg Z9-14:Ac+16 μg Z9-14:OH+42 μg Z11-16:Ac is suggested for use in monitoring traps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00994316
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  • 4
    Electronic Resource
    Electronic Resource
    Mating disruption for control of damage by codling moth in Virginia apple orchards (1993)
    Springer
    Entomologia experimentalis et applicata 67 (1993), S. 57-64 
    Details
    ISSN: 1570-7458
    Keywords: Cydia pomonella ; codling moth ; mating disruption ; (E,E)-8,10-dodecadien-1-ol ; pheromones ; apple ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyethylene dispensers (Shin Etsu) containing 172 ml of the sex pheromone, (E, E)-8,10-dodecadien-1-ol (63%), dodecenol (31%) and tetradecenol (6%), of codling moth (CM),Cydia pomonella (L.), were placed in apple orchards in Virginia. Two blocks of about 2 ha each were treated in 1989, and three in 1990. Dispensers were placed in trees at a density of 1000/ha shortly after apple bloom. Male orientation to pheromone traps was almost totally disrupted (a few males were captured at high population densities). In 1989, the Daleville pheromone-treated block had 0.9% and 0.8% CM-injured fruit in the center and edge, respectively; 0% and 39.5% injured fruit were found in the conventional control and abandoned blocks, respectively. The Criglersville orchard (‘organically’ managed, with high CM density) CM harvest injury was 16.0%, 16.5%, 34.5%, and 26.5% in the pheromone-treated center and edge, organic control and abandoned blocks, respectively. In 1990, the Daleville CM harvest injury was 4.7%, 7.3%, 1.1%, 0.3% and 58%, in the pheromone-treated center and edge, control center and edge, and abandoned blocks, respectively (possible reasons for the high injury in this block are discussed). Harvest injury in the Fincastle pheromone-treated and control blocks were 0.7% and 0%, respectively. The Criglersville orchard yielded 17%, 19% and 20% CM-injured fruit at harvest in the pheromone-treated, organic control and abandoned blocks, respectively. Pheromone release rate was calculated as 37 mg/ha/h in 1989.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02382717
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  • 5
    Electronic Resource
    Electronic Resource
    Mating disruption to control damage by leafrollers in virginia apple orchards (1993)
    Springer
    Entomologia experimentalis et applicata 67 (1993), S. 47-56 
    Details
    ISSN: 1570-7458
    Keywords: Platynota flavedana ; variegated leafroller ; Platynota ideausalis ; tufted apple bud moth ; Argyrotaenia velutinana ; redbanded leafroller ; mating disruption ; pheromones ; apple ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mating disruption for control of variegated leafroller (VLR),Platynota flavedana (Clemens), tufted apple bud moth (TBM),P. ideausalis Walker, and redbanded leafroller (RBL),Argyrotaenia velutinana (Walker), was studied in Virginia apple orchards in 1989 and 1990. In 1989, each dispenser (1000/ha) contained 190 mg of 67.2%E11–14:Ac, 28.8%Z11–14:Ac, 1.4%E11–14:OH, 0.6%Z11–14:OH, and 2%Z9–12:Ac (a putative generic leafroller disruption blend). Trap captures of VLR, TBM and RBL were reduced by 97%, 51% and 55%, respectively. Average leafroller injury in the interior and edge of the pheromone block was 3.8% and 2.7%, respectively. The conventional control and abandoned blocks had 0.05 and 27.5% injury, respectively. Dispensers containingE11–14:OH (70%) andZ11–14:OH (30%) (close to the natural blend of VLR), more effectively disrupted orientation to pheromone traps by bothPlatynota spp. than did the generic blend. In 1990, dispensers containing 150 mg ofE11–14:OH (70%) andZ11–14:OH (1000/ha) were placed in two 2-ha blocks and one 4-ha block. One pheromonetreated block was sprayed in August with phosmet for codling moth, not timed for leafrollers. Trap captures of VLR and TBM were reduced by almost 100% and 69%, respectively. RBL captures were not reduced by VLR pheromone permeation. Injury fromPlatynota spp. in pheromone block middles and edges ranged from 0.3–1.7% and 0.3–2.3%, respectively. Injury in conventional blocks ranged from 0–1.1%. RBL injury in pheromone block middles ranged from 0–6.1%, and in edges, 1.7–4.8%. Injury in control blocks ranged from 0–1.1%. Combined leafroller injury in an abandoned block was 18% (s.e. 0.3). Release rates averaged 30 and 32 mg/ha/h for the VLR and generic pheromone dispensers, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02382716
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  • 6
    Electronic Resource
    Electronic Resource
    Inter-specific avoidance of egg-associated semiochemicals in four tortricids (1993)
    Springer
    Cellular and molecular life sciences 49 (1993), S. 998-1001 
    Details
    ISSN: 1420-9071
    Keywords: Lepidoptera ; Tortricidae ; synomone ; pheromone ; behavior ; oviposition ; Lobesia botrana ; Cydia pomonella ; Cydia molesta ; Eupoecilia ambiguella ; fatty acids ; esters of fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Oviposition of four tortricid pests of vineyards (the European grapevine moth and the grape berry moth) and fruit orchards (the codling moth and the oriental fruit moth) is deterred by a blend of straight chain fatty acids and esters of fatty acids that have been identified in the eggs of one of them: the European grapevine moth (EGVM)Lobesia botrana. This is the first evidence of inter-specific recognition of an egg-like signal in moths. We demonstrate that oviposition site selection is influenced by population density, avoidance of deterrent being most important when females are isolated. Inter-specific egg recognition might be an important phenomenon, especially in species competing for a common food resource. We propose the term ‘oviposition regulating synomone’ for molecules and blends that affect the inter-specific spacing of eggs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02125648
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  • 7
    Electronic Resource
    Electronic Resource
    Development of a method for the identification of S alleles in Brassica oleracea based on digestion of PCR-amplified DNA with restriction endonucleases (1993)
    Springer
    Sexual plant reproduction 6 (1993), S. 133-138 
    Details
    ISSN: 1432-2145
    Keywords: Brassica oleracea ; Self-incompatibility ; S alleles ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The development of a technique for the identification of S alleles involved in self-incompatibility in Brassica oleracea which is based on the polymerase chain reaction (PCR) amplification of genomic DNA followed by restriction analysis is described. Primers homologous to conserved regions near to the 5′ and 3′ ends of the S coding sequence were used to amplify a number of members of the S multigene family. However, by designing a selective primer and using a higher temperature for annealing in the PCR, we were able to amplify certain members from the multigene family preferentially. These were considered to be the S-locus glycoprotein genes (SLG), since the patterns of restriction bands of the PCR products were shown to correspond to those of the SLG where sequence data were available. DNA samples from plants with certain S alleles were found not to amplify efficiently using the “selective” primers and high annealing temperature. This property, however, could be used as a means of distinguishing plants homozygous for these S alleles, as was demonstrated by an examination of a small F2 population that was segregating for the S5 and S29 alleles. In the investigation of the F2 population, it was found that preferential amplification of one of the alleles of the heterozygotes occurred when the “consensus” primers were used in the PCR. However, by using different primers, homologous to another region of the S sequence, we were able to amplify both alleles of the heterozygotes equally. The genotypes of the plants were determined by restriction analysis of PCR products and agreed with results based on pollen-tube growth tests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227658
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  • 8
    Electronic Resource
    Electronic Resource
    Phylogenetic analysis of Syntrophobacter wolinii reveals a relationship with sulfate-reducing bacteria (1993)
    Springer
    Archives of microbiology 160 (1993), S. 238-240 
    Details
    ISSN: 1432-072X
    Keywords: Syntrophobacter wolinii ; Syntrophic bacteria ; Sulfate ; reducing bacteria ; PCR ; 16S rRNA ; Phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 16S rRNA sequence analysis of Syntrophobacter wolinii was done by using PCR amplification of the 16S rRNA-genes from DNA isolated from the S. wolinii-Desulfovibrio sp. coculture. Phylogenetic analysis using the obtained sequence revealed that S. wolinii was not related to bacteria growing syntrophically on other fatty acids than propionate, but was related to sulfate-reducing bacteria. The closest related bacteria are Desulfomonile tiedjei and Desulfoarculus baarsii.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249130
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  • 9
    Electronic Resource
    Electronic Resource
    Trail and arena marking by caterpillars ofArchips cerasivoranus (lepidoptera: Tortricidae) (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1479-1489 
    Details
    ISSN: 1573-1561
    Keywords: Archips cerasivoranus ; Yponomeuta cagnagella ; trail following ; silk ; trail pheromone ; Lepidoptera ; Tortricidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The activity ofArchips cerasivoranus caterpillars is largely limited to their colonial silk web and trails. Silk pulled directly from the spinnerets of caterpillars and wound onto paper strips to form artificial trails elicited locomotion from the larvae. Trails made from extracts of silk and silk glands also elicited locomotion. These and other observations reported here indicate that the caterpillars are responsive to a water-soluble pheromone that is a component of the silk strand. Marker pheromones appear not to be secreted from other regions of the body, as has been reported for some other trail-following caterpillars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00984891
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  • 10
    Electronic Resource
    Electronic Resource
    DNA amplification fingerprinting of the Azolla-Anabaena symbiosis (1993)
    Springer
    Plant molecular biology 21 (1993), S. 363-373 
    Details
    ISSN: 1573-5028
    Keywords: eukaryotic ; arbitrary oligonucleotide primer ; PCR ; prokaryotic ; silver staining ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Azolla-Anabaena symbiosis has been used for centuries as a nitrogen biofertilizer in rice paddies. Genetic improvement of the symbiosis has been limited by the difficulty in identifying Azolla-Anabaena accessions and Anabaena azollae strains. The recently developed technique of DNA amplification fingerprinting (DAF) was applied to this problem. DAF uses single, short, oligonucleotide primers of arbitrary sequence to direct amplification of a characteristic set of DNA products by a thermostable DNA polymerase in a thermocycling reaction. The products are separated in polyacrylamide gels and detected by silver staining. DAF could easily distinguish and positively identify accessions of Azolla-Anabaena with DNA extracted from the intact symbioses. The contribution of prokaryotic Anabaena sequences to the fingerprint of the intact symbioses, however, ranged from 0 to 77%, depending on the primer sequence. Therefore, DNA extracted from the intact symbioses would not be suitable for Azolla taxonomy studies. The fingerprints of Anabaena strains isolated by sucrose gradient centrifugation from different species of Azolla could be easily distinguished, and DAF patterns were used to confirm the maternal pattern of transmission of Anabaena in a sexual hybrid. Template DNA extracted from roots was used to produce fingerprints for Azolla without interference from the microsymbiont. Comparison of the patterns from the parents and a hybrid gave strong evidence confirming sexual hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019951
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