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Transparency: issues are not that simple (2016)

Warren Pearce; Sarah Hartley; Brigitte Nerlich

Springer Nature

In: Nature

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Publikationsdatum: 2016-03-03

Beschreibung: Transparency: issues are not that simple Nature 531, 7592 (2016). doi:10.1038/531035d Authors: Warren Pearce, Sarah Hartley & Brigitte Nerlich We find Stephan Lewandowsky and Dorothy Bishop's framing of science governance to be overly simplistic and in need of a firmer evidence base ('Don't let transparency damage science'Nature529, 459–461;10.1038/529459a2016).The authors' analysis is biased by

Print ISSN: 0028-0836

Digitale ISSN: 1476-4687

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Publiziert von Springer Nature

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Materials, Vol. 9, Pages 276: In Vitro Testing of Scaffolds for Mesenchymal Stem Cell-Based Meniscus Tissue Engineering—Introducing a New Biocompatibility Scoring System (2016)

Felix Achatz; Richard Kujat; Christian Pfeifer; Matthias Koch; Michael Nerlich; Peter Angele; Johannes Zellner

MDPI Publishing

In: Materials

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Publikationsdatum: 2016-04-08

Beschreibung: A combination of mesenchymal stem cells (MSCs) and scaffolds seems to be a promising approach for meniscus repair. To facilitate the search for an appropriate scaffold material a reliable and objective in vitro testing system is essential. This paper introduces a new scoring for this purpose and analyzes a hyaluronic acid (HA) gelatin composite scaffold and a polyurethane scaffold in combination with MSCs for tissue engineering of meniscus. The pore quality and interconnectivity of pores of a HA gelatin composite scaffold and a polyurethane scaffold were analyzed by surface photography and Berliner-Blau-BSA-solution vacuum filling. Further the two scaffold materials were vacuum-filled with human MSCs and analyzed by histology and immunohistochemistry after 21 days in chondrogenic media to determine cell distribution and cell survival as well as proteoglycan production, collagen type I and II content. The polyurethane scaffold showed better results than the hyaluronic acid gelatin composite scaffold, with signs of central necrosis in the HA gelatin composite scaffolds. The polyurethane scaffold showed good porosity, excellent pore interconnectivity, good cell distribution and cell survival, as well as an extensive content of proteoglycans and collagen type II. The polyurethane scaffold seems to be a promising biomaterial for a mesenchymal stem cell-based tissue engineering approach for meniscal repair. The new score could be applied as a new standard for in vitro scaffold testing.

Digitale ISSN: 1996-1944

Thema: Maschinenbau

Publiziert von MDPI Publishing

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Materials, Vol. 9, Pages 381: Higher Ratios of Hyaluronic Acid Enhance Chondrogenic Differentiation of Human MSCs in a Hyaluronic Acid–Gelatin Composite Scaffold (2016)

Christian Pfeifer; Arne Berner; Matthias Koch; Werner Krutsch; Richard Kujat; Peter Angele; Michael Nerlich; Johannes Zellner

MDPI Publishing

In: Materials

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Publikationsdatum: 2016-05-18

Beschreibung: Mesenchymal stem cells (MSCs) seeded on specific carrier materials are a promising source for the repair of traumatic cartilage injuries. The best supportive carrier material has not yet been determined. As natural components of cartilage’s extracellular matrix, hyaluronic acid and collagen are the focus of biomaterial research. In order to optimize chondrogenic support, we investigated three different scaffold compositions of a hyaluronic acid (HA)-gelatin based biomaterial. Methods: Human MSCs (hMSCs) were seeded under vacuum on composite scaffolds of three different HA-gelatin ratios and cultured in chondrogenic medium for 21 days. Cell-scaffold constructs were assessed at different time points for cell viability, gene expression patterns, production of cartilage-specific extracellular matrix (ECM) and for (immuno-)histological appearance. The intrinsic transforming growth factor beta (TGF-beta) uptake of empty scaffolds was evaluated by determination of the TGF-beta concentrations in the medium over time. Results: No significant differences were found for cell seeding densities and cell viability. hMSCs seeded on scaffolds with higher ratios of HA showed better cartilage-like differentiation in all evaluated parameters. TGF-beta uptake did not differ between empty scaffolds. Conclusion: Higher ratios of HA support the chondrogenic differentiation of hMSCs seeded on a HA-gelatin composite scaffold.

Digitale ISSN: 1996-1944

Thema: Maschinenbau

Publiziert von MDPI Publishing

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Transparency: issues are not that simple (2016)

W. Pearce ; S. Hartley ; B. Nerlich

Nature Publishing Group (NPG)

In: Nature. 531(7592): 35. doi: 10.1038/531035d.

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Publikationsdatum: 2016-03-05

Beschreibung: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pearce, Warren -- Hartley, Sarah -- Nerlich, Brigitte -- England -- Nature. 2016 Mar 3;531(7592):35. doi: 10.1038/531035d.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉University of Nottingham, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26935688" target="_blank"〉PubMed〈/a〉

Schlagwort(e): Humans ; *Information Dissemination ; Research/*standards

Print ISSN: 0028-0836

Digitale ISSN: 1476-4687

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Publiziert von Nature Publishing Group (NPG)

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