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  • 1
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    Tetrodotoxin and saxitoxin in some Nassarius species (Nassarius Duméril, 1806) collected in Khanh Hoa waters (2017)
    Details
    Publication Date: 2021-05-19
    Description: Toxins of 5 species belonging to Nassarius collected in Khanh Hoa waters, including Nassarius siquijorensis, N. glans glans, N. livescens, N. pullus, and N. conoidalis conoidalis were detected. Among them, three species contained a high amount of toxin: 431.49 ± 206.50 MU/g in N. glans glans, 154.81 ± 85.02 MU/g in N. conoidalis conoidalis and 20.44 ± 9.77 MU/g in N. pullus. High-performance liquid chromatography with fluorometric detector (FPLC-FLD) revealed that the major toxin component was tetrodotoxin (TTX) 4epi-TTX, 4,9-anhydroTTX, whereas saxitoxin was not found in any specimen by this method.
    Description: Published
    Keywords: Gastropoda ; Nassarius ; Tetrodotoxin ; Saxitoxin ; Toxin ; TTX
    Repository Name: AquaDocs
    Type: Journal Contribution , Not Known
    Format: pp. 70-79
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  • 2
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    Investigation of protein patterns and antioxidant activity of collagen hydrolysates from skin of Fan-bellied leatherjacket Monacanthus chinensis by various enzymes (2019)
    Details
    Publication Date: 2021-05-19
    Description: Collagen extracted from the skin of fan-bellied leatherjacket was hydrolyzed and tested for antioxidant activity. The yields of acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were 14.8% and 19.6%, respectively, based on the wet weight of skin. The SDS-PAGE results showed that collagen from the skin of this fish consists of α1 and α2 chains with molecular weight (MW) approximately 100 kDa, identified as type I collagen. Hydrolysis of collagen by the other enzymes, such as papain, bromelain, pepsin and alcalase could produce the peptides with MW less than 28 kDa. Hydrolyzed collagen possessed the antioxidant activity with different levels and greater than that of normal collagen.
    Description: Published
    Keywords: Fan-bellied leatherjacket ; Antioxidant activity ; Hydrolyzed collagen ; Enzymes
    Repository Name: AquaDocs
    Type: Journal Contribution , Not Known
    Format: pp. 141 - 150
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  • 3
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    Certain properties of calcium hydroxyapatite from skipjack tuna bone, Katsuwonus pelamis (2019)
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    Publication Date: 2021-05-19
    Description: This paper is concerned with certain properties of calcium hydroxyapatite from skipjack tuna bone (Katsuwonus pelamis) which are by-products of fish export industry. Hydroxyapatite Ca10(PO4)6(OH)2 and β-tricalcium phosphate Ca3(PO4)2, the high-value compounds, have been successfully extracted from skipjack tuna bones. The bones were heated at different temperatures of 600oC, 900oC, 1200oC. While at 600oC hydroxyapatites were obtained with Ca/P ratio of 1.658, comparable to the value of 1.67 found in human bone; the hydroxyapatite crystals of average size of 0.25 µm were formed with the same size distribution. In case of heated bone samples at 900°C and 1200°C, the calcium formed were biphasic calcium phosphate composed of hydroxyapatite and β-tricalcium phosphate; the Ca/P ratio was between 1.660–1.665; the calcium crystals of more than 1 µm were highly porous and connected to each other in priority orientation of tube direction.
    Description: Published
    Keywords: Skipjack tuna bone ; Katsuwonus pelamis ; Calcium hydroxyapatite
    Repository Name: AquaDocs
    Type: Journal Contribution , Not Known
    Format: pp. 151-163
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  • 4
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    Preparation of nanohydroxyapatite from skipjack tuna bone (Katsuwonus pelamis) by alkaline hydrolysis and thermal calcination methods (2022)
    Details
    Publication Date: 2022-06-07
    Description: As the main component of bones and teeth, hydroxyapatite (HAp) is considered a vital biomaterial in many fields. Meanwhile, fishbone by-products are abundant sources of HAp. Therefore, this study was conducted to prepare nano HAp from skipjack tuna bone (Katsuwonus pelamis) using alkaline hydrolysis and thermal calcination methods. After pre-treatment of fish bones for removing organic impurities, the bone powders were under alkaline hydrolysis in 1 M NaOH solution at 250o C for 1 hour. This method allows to obtain HAp nanopowders with an average particle size of only 22 nm; whereas, when heating at 600o C for four hours, the calcium formed was on average larger (40 nm) in size than the alkaline hydrolysis product. The HAp nanopowders formed by alkaline hydrolysis had a molar ratio of Ca/P 1.929, higher than that of the calcinated sample (1.848). These Ca/P molar ratios prove that the nano-HAp powders are B-type biological hydroxyapatites confirmed by the FTIR spectrum. In addition, the heavy metal contents of calcium powders are detected within safety limits of regulatory requirements of Vietnam regulations on dietary supplements and functional foods.
    Description: Published
    Description: Refereed
    Keywords: Skipjack tuna bon ; Nano hydroxyapatite ; Thermal calcination ; Skipjack tuna ; Katsuwonus pelamis
    Repository Name: AquaDocs
    Type: Journal Contribution
    Format: 67-80
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  • 5
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    Composition and antioxidant activity of crude extract from squid Uroteuthis chinensis (2022)
    Details
    Publication Date: 2022-06-07
    Description: The antioxidant activities against DPPH free radical of crude extracts using ethyl acetatee and methanol from Uroteuthis Chinensis were studied muscles of, ranged from 13.43% to 41.13%. TLC and FT-IR analysis of several fractions methanol extracts showed the presence of amino acids, peptides, and proteins. SDS-PAGE result of some fractions of methanol extracts described the presence of proteins with a molecular weight of 19-220 kDa. The antioxidant activities of different segments of the Uroteuthis Chinensis were various.
    Description: Published
    Description: Refereed
    Keywords: Squid ; Uroteuthis chinensis ; Biochemistry ; Antioxidant
    Repository Name: AquaDocs
    Type: Journal Contribution
    Format: 81-91
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  • 6
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    Frequent occurrence of tetrodotoxin in the marine gastropod Nassarius glans causing a food poisoning in Khanh Hoa province, Vietnam in 2020 (2024)
    Details
    Publication Date: 2024-03-27
    Description: A fatal neurotoxic poisoning case happened in Khanh Hoa province in 2020 after eating a certain number of marine gastropods, later identified as Nassarius glans, one of the common marine gastropods in Vietnam. As the remaining causative food in the incident, 62 specimens were collected to examine tetrodotoxin toxicity individual variation and frequency of toxic specimens by using HILIC/MS-MS analysis. 100% of studied specimens exhibited toxicity (556 ± 821 MU/g) beyond the regulatory level of consumption (10 MU/g) for puffer(fish) recommended in Japan and extensive variation (18–4,046 MU/g). The result pointed out that only 5 g of soft tissue from N. glans (equivalent to 2–3 specimens) containing maximum toxicity detected in the present study may cause human death if consumed. Fhe first time, this study identified TTXs in the gastropods as a causative toxin in the poisoning in Vietnam. Moreover, 65.5% of studied specimens with high toxicity higher than 100 MU/g, including 16.1%, showing extremely high toxicity (〉 1,000 MU/g). The results suggested that this gastropod is quite dangerous for human consumption and should be alerted to public awareness.
    Description: Published
    Description: Refereed
    Keywords: Gastropod ; Nassarius glans ; Poisoning ; Tetrodotoxin
    Repository Name: AquaDocs
    Type: Journal Contribution
    Format: 203-208
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