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  • 1
    Electronic Resource
    Electronic Resource
    Protein Inhalation Powders: Spray Drying vs Spray Freeze Drying (1999)
    Springer
    Pharmaceutical research 16 (1999), S. 249-254 
    Details
    ISSN: 1573-904X
    Keywords: spray freeze drying ; spray drying ; dispersibility ; fine particle fraction ; liquid impingement ; cascade impaction ; aerodynamic particle size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. To develop a new technique, spray freeze drying, for preparing protein aerosol powders. Also, to compare the spray freeze-dried powders with spray-dried powders in terms of physical properties and aerosol performance. Methods. Protein powders were characterized using particle size analysis, thermogravimetric analysis, scanning electron microscopy, X-ray powder diffractometry, and specific surface area measurement. Aerosol performance of the powders was evaluated after blending with lactose carriers using a multi-stage liquid impinger or an Anderson cascade impactor. Two recombinant therapeutic proteins currently used for treating respiratory tract-related diseases, deoxyribonuclase (rhDNase) and anti-IgE monoclonal antibody (anti-IgE MAb), were employed and formulated with different carbohydrate excipients. Results. Through the same atomization but the different drying process, spray drying (SD) produced small (∼3 μm), dense particles, but SFD resulted in large (∼8−10 μm), porous particles. The fine particle fraction (FPF) of the spray freeze-dried powder was significantly better than that of the spray-dried powder, attributed to better aerodynamic properties. Powders collected from different stages of the cascade impactor were characterized, which confirmed the concept of aerodynamic particle size. Protein formulation played a major role in affecting the powder's aerosol performance, especially for the carbohydrate excipient of a high crystallization tendency. Conclusions. Spray freeze drying, as opposed to spray drying, produced protein particles with light and porous characteristics, which offered powders with superior aerosol performance due to favorable aerodynamic properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018828425184
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of Spray Drying and Subsequent Processing Conditions on Residual Moisture Content and Physical/Biochemical Stability of Protein Inhalation Powders (1998)
    Springer
    Pharmaceutical research 15 (1998), S. 768-775 
    Details
    ISSN: 1573-904X
    Keywords: spray drying ; residual moisture ; equilibrium moisture content ; relative humidity ; gravimetric moisture sorption isotherm ; protein aggregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. To understand the effect of spray drying and powder processing environments on the residual moisture content and aerosol performance of inhalation protein powders. Also, the long-term effect of storage conditions on the powder's physical and biochemical stability was presented. Methods. Excipient-free as well as mannitol-formulated powders of a humanized monoclonal antibody (anti-IgE) and recombinant human deoxyribonuclease (rhDNase) were prepared using a Buchi 190 model spray dryer. Residual moisture content and moisture uptake behavior of the powder were measured using thermal gravimetric analysis and gravimetric moisture sorption isotherm, respectively. Protein aggregation, the primary degradation product observed upon storage, was determined by size-exclusion HPLC. Aerosol performance of the dry powders was evaluated after blending with lactose carriers using a multi-stage liquid impinger (MSLI). Results. Spray-dried powders with a moisture level (~ 3%) equivalent to the freeze-dried materials could only be achieved using high-temperature spray-drying conditions, which were not favorable to large-male manufacturing, or subsequent vacuum drying. These dry powders would equilibrate with the subsequent processing and storage environments regardless of the manufacturing condition. As long as the relative humidity of air during processing and storage was lower than 50%, powders maintained their aerosol performance (fine particle fraction). However, powders stored under drier conditions exhibited better long-term protein biochemical stability. Conclusions. Manufacturing, powder processing, and storage environments affected powder's residual moisture level in a reversible fashion. Therefore, the storage condition determined powder's overall stability, but residual moisture had a greater impact on protein chemical stability than on powder physical stability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1011983322594
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  • 3
    Electronic Resource
    Electronic Resource
    The Effect of Formulation Excipients on Protein Stability and Aerosol Performance of Spray-Dried Powders of a Recombinant Humanized Anti-IgE Monoclonal Antibody1 (1999)
    Springer
    Pharmaceutical research 16 (1999), S. 350-358 
    Details
    ISSN: 1573-904X
    Keywords: aggregation ; glycation ; fine particle fraction ; protein formulation ; protein stability ; spray drying
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. To study the effect of trehalose, lactose, and mannitol on the biochemical stability and aerosol performance of spray-dried powders of an anti-IgE humanized monoclonal antibody. Methods. Protein aggregation of spray-dried powders stored at various temperature and relative humidity conditions was assayed by size exclusion chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis. Protein glycation was determined by isoelectric focusing and affinity chromatography. Crystallization was examined by X-ray powder diffraction. Aerosol performance was assessed as the fine particle fraction (FPF) of the powders blended with coarse carrier lactose, and was determined using a multiple stage liquid impinger. Results. Soluble protein aggregation consisting of non-covalent and disulfide-linked covalent dimers and trimers occurred during storage. Aggregate was minimized by formulation with trehalose at or above a molar ratio in the range of 300:1 to 500:1 (excipient:protein). However, the powders were excessively cohesive and unsuitable for aerosol administration. Lactose had a similar stabilizing effect, and the powders exhibited acceptable aerosol performance, but protein glycation was observed during storage. The addition of mannitol also reduced aggregation, while maintaining the FPF, but only up to a molar ratio of 200:1. Further increased mannitol resulted in crystallization, which had a detrimental effect on protein stability and aerosol performance. Conclusions. Protein stability was improved by formulation with carbohydrate. However, a balance must be achieved between the addition of enough stabilizer to improve protein biochemical stability without compromising blended powder aerosol performance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018805232453
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  • 4
    Electronic Resource
    Electronic Resource
    Spray-drying performance of a bench-top spray dryer for protein aerosol powder preparation (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 60 (1998), S. 301-309 
    Details
    ISSN: 0006-3592
    Keywords: cyclone design and configuration ; receiving vessel ; spray drying ; system design ; production yield ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The objective of this work was to improve a bench-top spray dryer's efficiency in both production recovery and throughput for preparing protein aerosol powders. A Büchi mini-spray dryer was used to prepare the powders of recombinant humanized anti-IgE antibody. The resulting powder's physical properties such as particle size, residual moisture, and morphology, along with its recovery and production rate was the basis of this development work. Mass balance suggests that approximately 10-20% of powder was lost in the exhaust air, consisting primarily of particles less than 2 μm. Also, significant loss (20-30%) occurred in the cyclone. Attempts were made to improve product recovery in the receiving vessel using dual-cyclone configurations, different cyclone designs, cyclones with anti-static treatment, and different receiver designs. System modifications such as replacing the original bag-filter unit with a vacuum system effectively reduced drying air flow resistance, allowing the protein to be dried at a lower inlet air temperature and the production scale to be increased. We concluded that the modified spray-drying system is advantageous over the original bench-top spray dryer. This improvement will be beneficial to early-stage research and development involving high-valued protein powders. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 301-309, 1998.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 5
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    Investigation of protein patterns and antioxidant activity of collagen hydrolysates from skin of Fan-bellied leatherjacket Monacanthus chinensis by various enzymes (2019)
    Details
    Publication Date: 2021-05-19
    Description: Collagen extracted from the skin of fan-bellied leatherjacket was hydrolyzed and tested for antioxidant activity. The yields of acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were 14.8% and 19.6%, respectively, based on the wet weight of skin. The SDS-PAGE results showed that collagen from the skin of this fish consists of α1 and α2 chains with molecular weight (MW) approximately 100 kDa, identified as type I collagen. Hydrolysis of collagen by the other enzymes, such as papain, bromelain, pepsin and alcalase could produce the peptides with MW less than 28 kDa. Hydrolyzed collagen possessed the antioxidant activity with different levels and greater than that of normal collagen.
    Description: Published
    Keywords: Fan-bellied leatherjacket ; Antioxidant activity ; Hydrolyzed collagen ; Enzymes
    Repository Name: AquaDocs
    Type: Journal Contribution , Not Known
    Format: pp. 141 - 150
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  • 6
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    Certain properties of calcium hydroxyapatite from skipjack tuna bone, Katsuwonus pelamis (2019)
    Details
    Publication Date: 2021-05-19
    Description: This paper is concerned with certain properties of calcium hydroxyapatite from skipjack tuna bone (Katsuwonus pelamis) which are by-products of fish export industry. Hydroxyapatite Ca10(PO4)6(OH)2 and β-tricalcium phosphate Ca3(PO4)2, the high-value compounds, have been successfully extracted from skipjack tuna bones. The bones were heated at different temperatures of 600oC, 900oC, 1200oC. While at 600oC hydroxyapatites were obtained with Ca/P ratio of 1.658, comparable to the value of 1.67 found in human bone; the hydroxyapatite crystals of average size of 0.25 µm were formed with the same size distribution. In case of heated bone samples at 900°C and 1200°C, the calcium formed were biphasic calcium phosphate composed of hydroxyapatite and β-tricalcium phosphate; the Ca/P ratio was between 1.660–1.665; the calcium crystals of more than 1 µm were highly porous and connected to each other in priority orientation of tube direction.
    Description: Published
    Keywords: Skipjack tuna bone ; Katsuwonus pelamis ; Calcium hydroxyapatite
    Repository Name: AquaDocs
    Type: Journal Contribution , Not Known
    Format: pp. 151-163
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  • 7
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    Antioxidant activity of extract from some squid species in Khanh Hoa (2021)
    Details
    Publication Date: 2021-09-30
    Description: The antioxidant activities against DPPH free radical of crude extracts using ethyl acetate and methanol from ink sac, muscles, and bones of 5 squid species (Uroteuthis chinensis, Uroteuthis sibogae, Uroteuthis duvaucelii, Sepia esculenta, Sepioteuthis lessoniana) collected in Khanh Hoa waters were investigated. These activities ranged from 4.21% (ethyl acetate extract from ink of U. chinensis) to 54.51% (methanol extract from muscle of S. esculenta.). The functional group analysis by infrared adsorption spectrum (FTIR) in these crude extracts revealed the absorption peaks of melanin in ink, polysaccharide in bone and protein in muscle extracts. In addition, the SDS-PAGE result of some methanol extracts showed the presence of proteins with a molecular weight of 30-150 kDa.
    Description: Published
    Description: Refereed
    Keywords: Squid ; Antioxidant ; Extract
    Repository Name: AquaDocs
    Type: Journal Contribution
    Format: pp. 187-198
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  • 8
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    Preparation of nanohydroxyapatite from skipjack tuna bone (Katsuwonus pelamis) by alkaline hydrolysis and thermal calcination methods (2022)
    Details
    Publication Date: 2022-06-07
    Description: As the main component of bones and teeth, hydroxyapatite (HAp) is considered a vital biomaterial in many fields. Meanwhile, fishbone by-products are abundant sources of HAp. Therefore, this study was conducted to prepare nano HAp from skipjack tuna bone (Katsuwonus pelamis) using alkaline hydrolysis and thermal calcination methods. After pre-treatment of fish bones for removing organic impurities, the bone powders were under alkaline hydrolysis in 1 M NaOH solution at 250o C for 1 hour. This method allows to obtain HAp nanopowders with an average particle size of only 22 nm; whereas, when heating at 600o C for four hours, the calcium formed was on average larger (40 nm) in size than the alkaline hydrolysis product. The HAp nanopowders formed by alkaline hydrolysis had a molar ratio of Ca/P 1.929, higher than that of the calcinated sample (1.848). These Ca/P molar ratios prove that the nano-HAp powders are B-type biological hydroxyapatites confirmed by the FTIR spectrum. In addition, the heavy metal contents of calcium powders are detected within safety limits of regulatory requirements of Vietnam regulations on dietary supplements and functional foods.
    Description: Published
    Description: Refereed
    Keywords: Skipjack tuna bon ; Nano hydroxyapatite ; Thermal calcination ; Skipjack tuna ; Katsuwonus pelamis
    Repository Name: AquaDocs
    Type: Journal Contribution
    Format: 67-80
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  • 9
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    Composition and antioxidant activity of crude extract from squid Uroteuthis chinensis (2022)
    Details
    Publication Date: 2022-06-07
    Description: The antioxidant activities against DPPH free radical of crude extracts using ethyl acetatee and methanol from Uroteuthis Chinensis were studied muscles of, ranged from 13.43% to 41.13%. TLC and FT-IR analysis of several fractions methanol extracts showed the presence of amino acids, peptides, and proteins. SDS-PAGE result of some fractions of methanol extracts described the presence of proteins with a molecular weight of 19-220 kDa. The antioxidant activities of different segments of the Uroteuthis Chinensis were various.
    Description: Published
    Description: Refereed
    Keywords: Squid ; Uroteuthis chinensis ; Biochemistry ; Antioxidant
    Repository Name: AquaDocs
    Type: Journal Contribution
    Format: 81-91
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  • 10
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    Frequent occurrence of tetrodotoxin in the marine gastropod Nassarius glans causing a food poisoning in Khanh Hoa province, Vietnam in 2020 (2024)
    Details
    Publication Date: 2024-03-27
    Description: A fatal neurotoxic poisoning case happened in Khanh Hoa province in 2020 after eating a certain number of marine gastropods, later identified as Nassarius glans, one of the common marine gastropods in Vietnam. As the remaining causative food in the incident, 62 specimens were collected to examine tetrodotoxin toxicity individual variation and frequency of toxic specimens by using HILIC/MS-MS analysis. 100% of studied specimens exhibited toxicity (556 ± 821 MU/g) beyond the regulatory level of consumption (10 MU/g) for puffer(fish) recommended in Japan and extensive variation (18–4,046 MU/g). The result pointed out that only 5 g of soft tissue from N. glans (equivalent to 2–3 specimens) containing maximum toxicity detected in the present study may cause human death if consumed. Fhe first time, this study identified TTXs in the gastropods as a causative toxin in the poisoning in Vietnam. Moreover, 65.5% of studied specimens with high toxicity higher than 100 MU/g, including 16.1%, showing extremely high toxicity (〉 1,000 MU/g). The results suggested that this gastropod is quite dangerous for human consumption and should be alerted to public awareness.
    Description: Published
    Description: Refereed
    Keywords: Gastropod ; Nassarius glans ; Poisoning ; Tetrodotoxin
    Repository Name: AquaDocs
    Type: Journal Contribution
    Format: 203-208
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