Publication Date:
1994-06-24
Description:
Glycosyl-phosphatidylinositol (GPI)-anchored proteins have been reported to reside in clusters collected over small membrane invaginations called caveolae. The detection of different GPI-anchored proteins with fluorescently labeled monoclonal antibodies showed that these proteins are not constitutively concentrated in caveolae; they enter these structures independently after cross-linking with polyclonal secondary antibodies. Analysis of the cell surface distribution of the GPI-anchored folate receptor by electron microscopy confirms these observations. Thus, multimerization of GPI-anchored proteins regulates their sequestration in caveolae, but in the absence of agents that promote clustering they are diffusely distributed over the plasma membrane.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mayor, S -- Rothberg, K G -- Maxfield, F R -- DK27083/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 1994 Jun 24;264(5167):1948-51.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7516582" target="_blank"〉PubMed〈/a〉
Keywords:
3T3 Cells
;
Animals
;
Antibodies, Monoclonal
;
Antigens, CD/analysis/immunology/metabolism
;
Antigens, CD55
;
Antigens, Surface/analysis/immunology/metabolism
;
Antigens, Thy-1
;
Carrier Proteins/analysis/immunology/*metabolism
;
Caveolin 1
;
*Caveolins
;
Cell Membrane/*metabolism/ultrastructure
;
Fluorescent Antibody Technique
;
Folate Receptors, GPI-Anchored
;
Folic Acid/metabolism
;
Glycosylphosphatidylinositols/analysis/*metabolism
;
Humans
;
Immunoglobulin G/metabolism
;
Membrane Glycoproteins/analysis/immunology/metabolism
;
Membrane Proteins/analysis
;
Mice
;
Microscopy, Electron
;
*Receptors, Cell Surface
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
