Sequestration of GPI-anchored proteins in caveolae triggered by cross-linking

Science. 1994 Jun 24;264(5167):1948-51. doi: 10.1126/science.7516582.

Abstract

Glycosyl-phosphatidylinositol (GPI)-anchored proteins have been reported to reside in clusters collected over small membrane invaginations called caveolae. The detection of different GPI-anchored proteins with fluorescently labeled monoclonal antibodies showed that these proteins are not constitutively concentrated in caveolae; they enter these structures independently after cross-linking with polyclonal secondary antibodies. Analysis of the cell surface distribution of the GPI-anchored folate receptor by electron microscopy confirms these observations. Thus, multimerization of GPI-anchored proteins regulates their sequestration in caveolae, but in the absence of agents that promote clustering they are diffusely distributed over the plasma membrane.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD / analysis
  • Antigens, CD / immunology
  • Antigens, CD / metabolism
  • Antigens, Surface / analysis
  • Antigens, Surface / immunology
  • Antigens, Surface / metabolism
  • CD55 Antigens
  • Carrier Proteins / analysis
  • Carrier Proteins / immunology
  • Carrier Proteins / metabolism*
  • Caveolin 1
  • Caveolins*
  • Cell Membrane / metabolism*
  • Cell Membrane / ultrastructure
  • Fluorescent Antibody Technique
  • Folate Receptors, GPI-Anchored
  • Folic Acid / metabolism
  • Glycosylphosphatidylinositols / analysis
  • Glycosylphosphatidylinositols / metabolism*
  • Humans
  • Immunoglobulin G / metabolism
  • Membrane Glycoproteins / analysis
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / metabolism
  • Membrane Proteins / analysis
  • Mice
  • Microscopy, Electron
  • Receptors, Cell Surface*
  • Thy-1 Antigens
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Surface
  • CD55 Antigens
  • Carrier Proteins
  • Caveolin 1
  • Caveolins
  • Folate Receptors, GPI-Anchored
  • Glycosylphosphatidylinositols
  • Immunoglobulin G
  • Membrane Glycoproteins
  • Membrane Proteins
  • Receptors, Cell Surface
  • Thy-1 Antigens
  • Folic Acid