Publication Date:
1986-07-11
Description:
Mammalian cell lines have been engineered to produce a secreted form of the AIDS retrovirus envelope glycoprotein. The recombinant protein has been isolated from growth-conditioned culture media and used to immunize animals. Antibodies directed against the recombinant molecule were found to react with the envelope glycoprotein produced in virus-infected cells. Furthermore, these antibodies were able to directly inactivate the AIDS retrovirus in a neutralization assay in vitro. The expression system reported here should provide sufficient quantities of the AIDS retrovirus envelope protein for biological and vaccination studies.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lasky, L A -- Groopman, J E -- Fennie, C W -- Benz, P M -- Capon, D J -- Dowbenko, D J -- Nakamura, G R -- Nunes, W M -- Renz, M E -- Berman, P W -- HL 33774-01/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1986 Jul 11;233(4760):209-12.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3014647" target="_blank"〉PubMed〈/a〉
Keywords:
Acquired Immunodeficiency Syndrome/*immunology
;
Animals
;
Antibodies, Viral/*immunology
;
Antigens, Viral/biosynthesis/*immunology
;
Cricetinae
;
Deltaretrovirus/*immunology
;
Guinea Pigs
;
HIV Antibodies
;
HIV Antigens
;
Humans
;
Immune Sera/immunology
;
Male
;
Neutralization Tests
;
Rabbits
;
Recombinant Proteins/biosynthesis/immunology
;
Viral Envelope Proteins/biosynthesis/*immunology
;
Viral Vaccines/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
