Publication Date:
1990-02-09
Description:
The Na+/H+ antiporter, which regulates intracellular pH in virtually all cells, is one of the best examples of a mitogen- and oncogene-activated membrane target whose activity rapidly changes on stimulation. The activating mechanism is unknown. A Na+/H+ antiporter complementary DNA fragment was expressed in Escherichia coli as a beta-galactosidase fusion protein, and a specific antibody to the fusion protein was prepared. Use of this antibody revealed that the Na+/H+ antiporter is a 110-kilodalton glycoprotein that is phosphorylated in growing cells. Mitogenic activation of resting hamster fibroblasts and A431 human epidermoid cells with epidermal growth factor, thrombin, phorbol esters, or serum, stimulated phosphorylation of the Na+/H+ antiporter with a time course similar to that of the rise in intracellular pH.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sardet, C -- Counillon, L -- Franchi, A -- Pouyssegur, J -- New York, N.Y. -- Science. 1990 Feb 9;247(4943):723-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Centre de Biochimie-CNRS, Nice, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2154036" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Blood
;
Carrier Proteins/genetics/*metabolism
;
Cell Line
;
Cricetinae
;
DNA/genetics
;
Epidermal Growth Factor/pharmacology
;
Escherichia coli/genetics
;
Fibroblasts/metabolism
;
Glycosylation
;
Growth Substances/*pharmacology
;
Humans
;
Immunoblotting
;
Mammary Tumor Virus, Mouse/genetics
;
Molecular Weight
;
Phosphorylation
;
Promoter Regions, Genetic
;
Recombinant Fusion Proteins/metabolism
;
Sodium-Hydrogen Antiporter
;
Thrombin/pharmacology
;
Transfection
;
beta-Galactosidase/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
