Publication Date:
2000-03-17
Description:
The initiation sites of bidirectional synthesis at the DNA replication origin located at the 3' end of the human lamin B2 gene were investigated. RNA-primed nascent DNA molecules were subjected to second-strand synthesis with appropriate primers, amplified by ligation-mediated polymerase chain reaction, and size fractionated. Evidence for precise start sites was obtained. Exploration of close to 1 kilobase, coupled to inhibition of Okazaki fragment synthesis, demonstrates that the leading strands initiate at precise nucleotides on either helix, overlapping by three base pairs, within the area bound to a protein complex possibly analogous to the prereplicative complex of yeast.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Abdurashidova, G -- Deganuto, M -- Klima, R -- Riva, S -- Biamonti, G -- Giacca, M -- Falaschi, A -- New York, N.Y. -- Science. 2000 Mar 17;287(5460):2023-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Molecular Biology and Molecular Medicine Units, International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34012 Trieste, Italy.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10720330" target="_blank"〉PubMed〈/a〉
Keywords:
AT Rich Sequence
;
Base Sequence
;
DNA/biosynthesis
;
DNA Primers/metabolism
;
*DNA Replication
;
Emetine/pharmacology
;
G1 Phase
;
HeLa Cells
;
Humans
;
*Lamin Type B
;
Lamins
;
Molecular Sequence Data
;
Nuclear Proteins/*genetics
;
Protein Binding
;
*Replication Origin
;
S Phase
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
