Publication Date:
1999-03-19
Description:
Activity shapes the structure of neurons and their circuits. Two-photon imaging of CA1 neurons expressing enhanced green fluorescent protein in developing hippocampal slices from rat brains was used to characterize dendritic morphogenesis in response to synaptic activity. High-frequency focal synaptic stimulation induced a period (longer than 30 minutes) of enhanced growth of small filopodia-like protrusions (typically less than 5 micrometers long). Synaptically evoked growth was long-lasting and localized to dendritic regions close (less than 50 micrometers) to the stimulating electrode and was prevented by blockade of N-methyl-D-aspartate receptors. Thus, synaptic activation can produce rapid input-specific changes in dendritic structure. Such persistent structural changes could contribute to the development of neural circuitry.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Maletic-Savatic, M -- Malinow, R -- Svoboda, K -- New York, N.Y. -- Science. 1999 Mar 19;283(5409):1923-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10082466" target="_blank"〉PubMed〈/a〉
Keywords:
2-Amino-5-phosphonovalerate/pharmacology
;
Animals
;
Culture Techniques
;
Dendrites/drug effects/physiology/*ultrastructure
;
Electric Stimulation
;
Excitatory Amino Acid Antagonists/pharmacology
;
Green Fluorescent Proteins
;
Hippocampus/cytology
;
Luminescent Proteins/genetics
;
Microscopy/methods
;
Morphogenesis/drug effects
;
Pseudopodia/drug effects/physiology/*ultrastructure
;
Pyramidal Cells/physiology/*ultrastructure/virology
;
Rats
;
Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors/*physiology
;
Sindbis Virus/genetics/physiology
;
Synapses/*physiology/ultrastructure
;
Synaptic Membranes/physiology
;
Synaptic Transmission
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
