Publication Date:
2013-11-10
Description:
Mitotic chromosomes are among the most recognizable structures in the cell, yet for over a century their internal organization remains largely unsolved. We applied chromosome conformation capture methods, 5C and Hi-C, across the cell cycle and revealed two distinct three-dimensional folding states of the human genome. We show that the highly compartmentalized and cell type-specific organization described previously for nonsynchronous cells is restricted to interphase. In metaphase, we identified a homogenous folding state that is locus-independent, common to all chromosomes, and consistent among cell types, suggesting a general principle of metaphase chromosome organization. Using polymer simulations, we found that metaphase Hi-C data are inconsistent with classic hierarchical models and are instead best described by a linearly organized longitudinally compressed array of consecutive chromatin loops.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040465/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040465/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Naumova, Natalia -- Imakaev, Maxim -- Fudenberg, Geoffrey -- Zhan, Ye -- Lajoie, Bryan R -- Mirny, Leonid A -- Dekker, Job -- HG003143/HG/NHGRI NIH HHS/ -- R01 HG003143/HG/NHGRI NIH HHS/ -- U54CA143874/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2013 Nov 22;342(6161):948-53. doi: 10.1126/science.1236083. Epub 2013 Nov 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School (UMMS), 368 Plantation Street, Worcester, MA 01605-0103, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24200812" target="_blank"〉PubMed〈/a〉
Keywords:
Biopolymers/chemistry
;
Cell Cycle/genetics
;
Chromatin/chemistry
;
Chromosomes, Human, Pair 21/*chemistry
;
HeLa Cells
;
Humans
;
Metaphase/genetics
;
Mitosis/*genetics
;
Models, Chemical
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
