Publication Date:
2005-06-04
Description:
Despite the greater information content of genomic DNA, ancient DNA studies have largely been limited to the amplification of mitochondrial sequences. Here we describe metagenomic libraries constructed with unamplified DNA extracted from skeletal remains of two 40,000-year-old extinct cave bears. Analysis of approximately 1 megabase of sequence from each library showed that despite significant microbial contamination, 5.8 and 1.1% of clones contained cave bear inserts, yielding 26,861 base pairs of cave bear genome sequence. Comparison of cave bear and modern bear sequences revealed the evolutionary relationship of these lineages. The metagenomic approach used here establishes the feasibility of ancient DNA genome sequencing programs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Noonan, James P -- Hofreiter, Michael -- Smith, Doug -- Priest, James R -- Rohland, Nadin -- Rabeder, Gernot -- Krause, Johannes -- Detter, J Chris -- Paabo, Svante -- Rubin, Edward M -- T32 HL07279/HL/NHLBI NIH HHS/ -- U1 HL66681B/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 2005 Jul 22;309(5734):597-9. Epub 2005 Jun 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉United States Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15933159" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cloning, Molecular
;
Computational Biology
;
DNA/genetics/history
;
Dogs/genetics
;
*Genome
;
Genomic Library
;
History, Ancient
;
Molecular Sequence Data
;
Phylogeny
;
Sequence Alignment
;
*Sequence Analysis, DNA
;
Ursidae/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
