Publication Date:
2004-01-24
Description:
What are the components that control the assembly of subcellular organelles in eukaryotic cells? Although membranes can clearly be distorted by cytosolic factors, very little is known about the intrinsic mechanisms that control the biogenesis, shape, and organization of organellar membranes. Here, we found that the unconventional phospholipid lysobisphosphatidic acid (LBPA) could induce the formation of multivesicular liposomes that resembled the multivesicular endosomes that exist where this lipid is found in vivo. This process depended on the same pH gradient that exists across endosome membranes in vivo and was selectively controlled by Alix. In turn, Alix regulated the organization of LBPA-containing endosomes in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Matsuo, Hirotami -- Chevallier, Julien -- Mayran, Nathalie -- Le Blanc, Isabelle -- Ferguson, Charles -- Faure, Julien -- Blanc, Nathalie Sartori -- Matile, Stefan -- Dubochet, Jacques -- Sadoul, Remy -- Parton, Robert G -- Vilbois, Francis -- Gruenberg, Jean -- New York, N.Y. -- Science. 2004 Jan 23;303(5657):531-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva 4, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14739459" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Annexin A2/metabolism
;
Arylsulfonates/metabolism
;
Calcium-Binding Proteins/genetics/*metabolism
;
Carrier Proteins/genetics/*metabolism
;
Cell Cycle Proteins
;
Cell Line
;
Coloring Agents/metabolism
;
Cytosol/metabolism
;
Endocytosis
;
Endosomal Sorting Complexes Required for Transport
;
Endosomes/*metabolism/ultrastructure
;
HeLa Cells
;
Humans
;
Hydrogen-Ion Concentration
;
Lipid Bilayers
;
Liposomes/*metabolism
;
Lysophospholipids/chemistry/*metabolism
;
Membrane Glycoproteins/metabolism
;
Molecular Structure
;
Monoglycerides
;
RNA Interference
;
RNA, Small Interfering/metabolism
;
Vesicular stomatitis Indiana virus/physiology
;
Viral Envelope Proteins/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
