Publication Date:
2001-12-18
Description:
The pore-forming subunits of canonical voltage-gated sodium and calcium channels are encoded by four repeated domains of six-transmembrane (6TM) segments. We expressed and characterized a bacterial ion channel (NaChBac) from Bacillus halodurans that is encoded by one 6TM segment. The sequence, especially in the pore region, is similar to that of voltage-gated calcium channels. The expressed channel was activated by voltage and was blocked by calcium channel blockers. However, the channel was selective for sodium. The identification of NaChBac as a functionally expressed bacterial voltage-sensitive ion-selective channel provides insight into both voltage-dependent activation and divalent cation selectivity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ren, D -- Navarro, B -- Xu, H -- Yue, L -- Shi, Q -- Clapham, D E -- New York, N.Y. -- Science. 2001 Dec 14;294(5550):2372-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Children's Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11743207" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Motifs
;
Amino Acid Sequence
;
Animals
;
Bacillus/*chemistry/genetics/metabolism
;
*Bacterial Proteins
;
CHO Cells
;
COS Cells
;
Calcium/metabolism
;
Calcium Channel Blockers/pharmacology
;
Calcium Channels/chemistry/metabolism
;
Cricetinae
;
Dihydropyridines/pharmacology
;
Genes, Bacterial
;
Ion Channel Gating
;
Membrane Potentials
;
Molecular Sequence Data
;
Molecular Weight
;
Open Reading Frames
;
Patch-Clamp Techniques
;
Protein Structure, Tertiary
;
Recombinant Proteins/metabolism
;
Sodium/*metabolism
;
Sodium Channels/chemistry/*genetics/*metabolism
;
Tetrodotoxin/pharmacology
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
