Publication Date:
2013-06-14
Description:
The tips of mammalian digits can regenerate after amputation, like those of amphibians. It is unknown why this capacity is limited to the area associated with the nail. Here we show that nail stem cells (NSCs) reside in the proximal nail matrix and that the mechanisms governing NSC differentiation are coupled directly with their ability to orchestrate digit regeneration. Early nail progenitors undergo Wnt-dependent differentiation into the nail. After amputation, this Wnt activation is required for nail regeneration and also for attracting nerves that promote mesenchymal blastema growth, leading to the regeneration of the digit. Amputations proximal to the Wnt-active nail progenitors result in failure to regenerate the nail or digit. Nevertheless, beta-catenin stabilization in the NSC region induced their regeneration. These results establish a link between NSC differentiation and digit regeneration, and suggest that NSCs may have the potential to contribute to the development of novel treatments for amputees.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936678/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936678/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Takeo, Makoto -- Chou, Wei Chin -- Sun, Qi -- Lee, Wendy -- Rabbani, Piul -- Loomis, Cynthia -- Taketo, M Mark -- Ito, Mayumi -- 1R01AR059768-01A1/AR/NIAMS NIH HHS/ -- 5P30CA0016087-32/CA/NCI NIH HHS/ -- P30 CA016087-30/CA/NCI NIH HHS/ -- R01 AR059768/AR/NIAMS NIH HHS/ -- S10 RR023704-01A1/RR/NCRR NIH HHS/ -- England -- Nature. 2013 Jul 11;499(7457):228-32. doi: 10.1038/nature12214. Epub 2013 Jun 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉The Ronald O. Perelman Department of Dermatology, New York University, School of Medicine, New York, New York 10016, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23760480" target="_blank"〉PubMed〈/a〉
Keywords:
Amputation
;
Animals
;
Bone and Bones/cytology/metabolism
;
Cell Differentiation
;
Cells, Cultured
;
Epithelium/metabolism
;
Extremities/growth & development/innervation/*physiology
;
Hoof and Claw/cytology/*growth & development/metabolism
;
Mesoderm/cytology/metabolism
;
Mice
;
Regeneration/*physiology
;
Stem Cells/cytology/metabolism
;
Wnt Proteins/*metabolism
;
Wnt Signaling Pathway
;
beta Catenin/genetics/metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
