Publication Date:
2010-11-16
Description:
The chemotaxis signalling network in Escherichia coli that controls the locomotion of bacteria is a classic model system for signal transduction. This pathway modulates the behaviour of flagellar motors to propel bacteria towards sources of chemical attractants. Although this system relaxes to a steady state in response to environmental changes, the signalling events within the chemotaxis network are noisy and cause large temporal variations of the motor behaviour even in the absence of stimulus. That the same signalling network governs both behavioural variability and cellular response raises the question of whether these two traits are independent. Here, we experimentally establish a fluctuation-response relationship in the chemotaxis system of living bacteria. Using this relationship, we demonstrate the possibility of inferring the cellular response from the behavioural variability measured before stimulus. In monitoring the pre- and post-stimulus switching behaviour of individual bacterial motors, we found that variability scales linearly with the response time for different functioning states of the cell. This study highlights that the fundamental relationship between fluctuation and response is not constrained to physical systems at thermodynamic equilibrium but is extensible to living cells. Such a relationship not only implies that behavioural variability and cellular response can be coupled traits, but it also provides a general framework within which we can examine how the selection of a network design shapes this interdependence.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230254/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230254/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Park, Heungwon -- Pontius, William -- Guet, Calin C -- Marko, John F -- Emonet, Thierry -- Cluzel, Philippe -- 1U54CA143869-01/CA/NCI NIH HHS/ -- P50 GM081892/GM/NIGMS NIH HHS/ -- P50 GM081892-04/GM/NIGMS NIH HHS/ -- R01 AI059195-03/AI/NIAID NIH HHS/ -- R01AI059195-03/AI/NIAID NIH HHS/ -- U54 CA143869/CA/NCI NIH HHS/ -- U54 CA143869-01/CA/NCI NIH HHS/ -- England -- Nature. 2010 Dec 9;468(7325):819-23. doi: 10.1038/nature09551. Epub 2010 Nov 14.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉The James Franck Institute, The Institute for Biophysical Dynamics, and The Department of Physics, University of Chicago, Chicago, Illinois 60637, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21076396" target="_blank"〉PubMed〈/a〉
Keywords:
Aspartic Acid/metabolism/pharmacology
;
Calibration
;
Chemotaxis/drug effects/*physiology
;
Chromatography, High Pressure Liquid
;
*Environment
;
Escherichia coli/*cytology/drug effects/*physiology
;
Flagella/drug effects/physiology
;
Molecular Motor Proteins/metabolism
;
Rotation
;
*Signal Transduction/drug effects
;
Stochastic Processes
;
Time Factors
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
