Publication Date:
2010-06-22
Description:
Eukaryotic cells spatially organize mRNA processes such as translation and mRNA decay. Much less is clear in bacterial cells where the spatial distribution of mature mRNA remains ambiguous. Using a sensitive method based on quantitative fluorescence in situ hybridization, we show here that in Caulobacter crescentus and Escherichia coli, chromosomally expressed mRNAs largely display limited dispersion from their site of transcription during their lifetime. We estimate apparent diffusion coefficients at least two orders of magnitude lower than expected for freely diffusing mRNA, and provide evidence in C. crescentus that this mRNA localization restricts ribosomal mobility. Furthermore, C. crescentus RNase E appears associated with the DNA independently of its mRNA substrates. Collectively, our findings show that bacteria can spatially organize translation and, potentially, mRNA decay by using the chromosome layout as a template. This chromosome-centric organization has important implications for cellular physiology and for our understanding of gene expression in bacteria.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896451/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896451/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Montero Llopis, Paula -- Jackson, Audrey F -- Sliusarenko, Oleksii -- Surovtsev, Ivan -- Heinritz, Jennifer -- Emonet, Thierry -- Jacobs-Wagner, Christine -- GM065835/GM/NIGMS NIH HHS/ -- R01 GM065835/GM/NIGMS NIH HHS/ -- R01 GM065835-07/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2010 Jul 1;466(7302):77-81. doi: 10.1038/nature09152. Epub 2010 Jun 20.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20562858" target="_blank"〉PubMed〈/a〉
Keywords:
Bacterial Proteins/genetics
;
Caulobacter crescentus/cytology/*genetics/*metabolism
;
Chaperonins/genetics
;
Chromosomes, Bacterial/genetics/metabolism
;
DNA, Bacterial/metabolism
;
Diffusion
;
Endoribonucleases/metabolism
;
Escherichia coli/cytology/*genetics/*metabolism
;
Gene Expression Regulation, Bacterial
;
In Situ Hybridization, Fluorescence
;
Lac Operon/genetics
;
Protein Biosynthesis
;
RNA Stability
;
*RNA Transport
;
RNA, Bacterial/analysis/genetics/*metabolism
;
RNA, Messenger/analysis/genetics/metabolism
;
Ribosomes/metabolism
;
Transcription, Genetic/genetics
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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