ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Treatment of cotyledons of 4-day-old cucumber (Cucumis sativus L. cv. Tokiwa-jibae) seedlings with cycloheximide (0.3 mM) inhibited protein synthesis in the cotyledons by 80%. In spite of this inhibition, the cycloheximide treatment induced a marked increase in the capacity of the alternative respiration in mitochondria, accompanied by an increase in the contribution of the pathway to the total respiration. In contrast, the activity of the cytochrome pathway was reduced by cycloheximide treatment. As a result, the total respiration was almost the same in mitochondria from cycloheximide-treated cotyledons and untreated cotyledons. Activities of some mitochondrial enzymes examined were also similar. Mitochondrial proteins synthesized during the treatment were separated by two-dimensional gel-electrophoresis and examined by fluorography. No new spots appeared and no spots disappeared on the fluorograms, but the labeling intensity of some polypeptides showed a relative increase or decrease as the result of cycloheximide treatment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1991.tb02481.x
