ISSN:
1436-5073
Keywords:
chemiluminescence
;
luminol
;
immobilized reagent
;
solidstate
;
flow injection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract To facilitate the application of luminol chemiluminescence in analysis, several approaches are investigated to provide the reagents in immobilized or solid-state format. The approaches are demonstrated with flow injection systems. Luminol is covalently bound or adsorbed to the surface of small support particles and packed into flow-through reactor/detector cells. The catalyst can be either covalently immobilized heme-containing species or a positively-biased electrode in an electrochemical cell. Peroxide can be obtained electrochemically at a negatively-biased electrode. These immobilized reagent systems can be combined to yield single-channel flow systems for determination of hydrogen peroxide (0.15μM detection limit) or luminol (0.1 nM detection limit).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01236108
