Abstract
To facilitate the application of luminol chemiluminescence in analysis, several approaches are investigated to provide the reagents in immobilized or solid-state format. The approaches are demonstrated with flow injection systems. Luminol is covalently bound or adsorbed to the surface of small support particles and packed into flow-through reactor/detector cells. The catalyst can be either covalently immobilized heme-containing species or a positively-biased electrode in an electrochemical cell. Peroxide can be obtained electrochemically at a negatively-biased electrode. These immobilized reagent systems can be combined to yield single-channel flow systems for determination of hydrogen peroxide (0.15μM detection limit) or luminol (0.1 nM detection limit).
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Nieman, T.A. Immobilized and solid-state reagent systems for luminol chemiluminescence in flow systems. Mikrochim Acta 96, 239–247 (1988). https://doi.org/10.1007/BF01236108
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DOI: https://doi.org/10.1007/BF01236108