ISSN:
1436-5073
Keywords:
electrogenerated Chemiluminescence
;
Chemiluminescence
;
luminol
;
Ru(bpy) 3 2+
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Chemiluminescence emission intensity is modulated by modulating the potential of a working electrode which is used to generate a key species in the electrogenerated Chemiluminescence (ECL) reaction. The emission is monitored synchronously using a lock-in amplifier. The reactions used in the characterization are luminol with hydrogen peroxide and tris(2,2′-bipyridyl)ruthenium (II) (or Ru(bpy) 3 2+ ) with oxalate. Modulation widths of ± 50 mV yield maximum signals for luminol when centered at 0.45 V (vs Ag/AgCl) and for Ru(bpy) 3 2+ when centered at 1.05 V. The resulting signal decreases with increasing modulation frequency and shows that luminol/H2O2 is a faster ECL system than Ru(bpy) 3 2+ /oxalate. Working curves for luminol and for oxalate have essentially the same linear range and slope with the modulated potential approach as with a DC electrode potential. This approach provides capability for differentiating the analytical signal from constant background emission or stray light.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01243623
