Skip to main content
SpringerLink
Log in

Modulated potential electrogenerated chemiluminescence of luminol and Ru(bpy) 2+3

  • Original Papers
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

Chemiluminescence emission intensity is modulated by modulating the potential of a working electrode which is used to generate a key species in the electrogenerated Chemiluminescence (ECL) reaction. The emission is monitored synchronously using a lock-in amplifier. The reactions used in the characterization are luminol with hydrogen peroxide and tris(2,2′-bipyridyl)ruthenium (II) (or Ru(bpy) 2+3 ) with oxalate. Modulation widths of ± 50 mV yield maximum signals for luminol when centered at 0.45 V (vs Ag/AgCl) and for Ru(bpy) 2+3 when centered at 1.05 V. The resulting signal decreases with increasing modulation frequency and shows that luminol/H2O2 is a faster ECL system than Ru(bpy) 2+3 /oxalate. Working curves for luminol and for oxalate have essentially the same linear range and slope with the modulated potential approach as with a DC electrode potential. This approach provides capability for differentiating the analytical signal from constant background emission or stray light.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. T. A. Nieman,Chemiluminescence, Overview of Techniques, in:Encyclopaedia of Analytical Science, Academic Press, New York.

  2. J. Kankare,Chemiluminescence, Electrogenerated Chemiluminescence, in:Encyclopaedia of Analytical Science, Academic Press, New York.

  3. G. M. Greenway,Trends Anal. Chem. 1990,9, 200.

    Google Scholar 

  4. T. Downey, T. A. Nieman,Anal. Chem. 1992,64, 261.

    Google Scholar 

  5. J. S. Littig, T. A. Nieman,Anal. Chem. 1992,64, 1140.

    Google Scholar 

  6. S. Sakura, J. Terao,Anal. Chim. Acta 1992,262, 217.

    Google Scholar 

  7. K. Uchikura, M. Kirisawa, A. Sugii,Anal. Sci. 1993,9, 121.

    Google Scholar 

  8. W. Y. Lee, T. A. Nieman,J. Chromatogr. 1994,659, 111.

    Google Scholar 

  9. G. F. Blackburn, H. P. Shah, J. H. Kenton, J. Leland, R. A. Kamin, J. Link, J. Peterman, M. J. Powell, A. Shah, D. B. Talley, S. K. Tyagi, E. Wilkens, T. G. Wu, R. J. Massey,Clin. Chem. 1991,37, 1534.

    Google Scholar 

  10. M. Aizawa, M. Tanka, Y. Ikariyama, H. Shinohara,J. Biolum. Chemilum. 1989,4, 535.

    Google Scholar 

  11. N. Egashira, N. Kondo, Y. Kurauchi, K. Ohga,Denki Kagaku 1992,60, 1148.

    Google Scholar 

  12. B. Epstein, T. Kuwana,Photochem. Photobiol. 1965,4, 1157.

    Google Scholar 

  13. I. Rubinstein, A. J. Bard,J. Am. Chem. Soc. 1983,55, 1580.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, 61801-3792, Urbana, IL, USA

    Glenn P. Jirka & Timothy A. Nieman

Authors
  1. Glenn P. Jirka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Timothy A. Nieman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jirka, G.P., Nieman, T.A. Modulated potential electrogenerated chemiluminescence of luminol and Ru(bpy) 2+3 . Mikrochim Acta 113, 339–347 (1994). https://doi.org/10.1007/BF01243623

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01243623

Key words

Search

Navigation