ISSN:
1435-232X
Keywords:
genotype
;
N-acetyltransferase
;
polymorphism
;
polymerase chain reaction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary PolymorphicN-acetyltransferase in human liver catalyzesN-acetylation of various arylamine-containing drugs and environmental chemicals. To accelerate the pharmacogenetic and ecogenetic studies ofN-acetyltransferase polymorphism, we have developed a rapid and simple method for genotyping using a polymerase chain reaction based restriction fragment length polymorphism. This method distinguishes four kinds of allele of theN-acetyltransferase gene using a single polymerase chain reaction starting with a set of primers, followed by successiveAsp718,BamHI andTaqI digestions, and then running the samples on a single electrophoresis lane. This method allows us to determine ten different genotypes easily and reliably.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01883706
