ISSN:
1617-4623
Keywords:
Dopa decarboxylase
;
P element
;
Transformation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Expression of the gene which encodes dopa decarboxylase in Drosophila melanogaster (Ddc) is temporally controlled. The variant strain Ddc +4 shows an altered pattern of enzyme activity during development compared with the standard Canton S laboratory strain. An examination of the DNA sequences which might control the expression of the variant gene was undertaken by reintroducing a cloned genomic fragment containing Ddc +4 into Drosophila via P element mediated genetic transformation. The analogous fragment from the Canton S strain was reintroduced as a control. Despite a generally reduced expression in one transformed line, all the reintegated Ddc alleles revealed temporal patterns of Ddc expression characteristic of the strain from which the transforming DNA had originally been derived. Thus, we conclude that the essential information of the variant Ddc +4 phenotype was included on a fragment which extended 2.9 kb upstream of the cap site for Ddc mRNA and 0.9 kb downstream of the poly(A) addition site.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00331613
