ISSN:
1617-4623
Keywords:
Soybean-Rhizobium symbiosis
;
Nodulins
;
cDNA clones
;
RNA analysis
;
DNA sequencing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Nine unique nodulin cDNA clones from soybean have been characterized with regard to the size of the RNA and the corresponding protein products. Based on the sequence homology between clones C51 and E27 and the multiple RNA species corresponding to clones D41 and E41, it is suggested that some of the nodulin genes represent members of small gene families. The amino acid sequence deduced from the nucleotide sequence of clones C51 and E27 revealed the presence of a “signal peptide” and no “stop transfer” signal, typical of membrane proteins, suggesting that the proteins encoded by these clones are localized in organelles and as such probably involved in ureide biosynthesis (Boland et al. 1982; Schubert and Boland 1984). Based on the timing of appearance of RNA corresponding to the nodulin clones and the pattern of their accumulation, at least three sets of nodulin genes are being represented here. Al1 the nodulin RNAs examined were made in Fix- nodules formed by strain Ag168 (which does not make Cl component of nitrogenase) at a level comparable to that in Fix+ nodules and at a very reduced level in Fix- nodules formed by strain HS124 (which show very few infected cells). It is concluded that all the nodulin genes examined here are induced independent of nitrogenase activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00422065
