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The use of antibiotics in the culture of non-sterile plant protoplasts (1973)

Watts, J. W. ; King, Janet M.

Springer

Planta 113 (1973), S. 271-277

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ISSN: 1432-2048

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The use of antibiotics to control infections in cultures of protoplasts of leaf mesophyll cells has been examined. The antifungal agents nystatin and amphotericin B were non-toxic to protoplasts at concentrations that controlled fungal growth (25 units and 2.5 μg/ml respectively). Of the antibacterial agents examined, only carbenicillin and, to a lesser extent, gentamicin were active against the bacteria usually encountered whilst still permitting normal protoplast metabolism and regeneration. The most satisfactory control of contaminating microorganisms was obtained with a combination of nystatin (25 units/ml) or amphotericin B (2.5 μg/ml) and carbenicillin (250 μg/ml).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00390514

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