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The use of antibiotics in the culture of non-sterile plant protoplasts

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Summary

The use of antibiotics to control infections in cultures of protoplasts of leaf mesophyll cells has been examined. The antifungal agents nystatin and amphotericin B were non-toxic to protoplasts at concentrations that controlled fungal growth (25 units and 2.5 μg/ml respectively). Of the antibacterial agents examined, only carbenicillin and, to a lesser extent, gentamicin were active against the bacteria usually encountered whilst still permitting normal protoplast metabolism and regeneration. The most satisfactory control of contaminating microorganisms was obtained with a combination of nystatin (25 units/ml) or amphotericin B (2.5 μg/ml) and carbenicillin (250 μg/ml).

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Authors and Affiliations

  1. John Innes Institute, Colney Lane, NOR 70 F, Norwich, U. K.

    J. W. Watts & Janet M. King

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  1. J. W. Watts
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  2. Janet M. King
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Watts, J.W., King, J.M. The use of antibiotics in the culture of non-sterile plant protoplasts. Planta 113, 271–277 (1973). https://doi.org/10.1007/BF00390514

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  • DOI: https://doi.org/10.1007/BF00390514

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