ISSN:
1432-2099
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Summary Wild type diploid yeast, Saccharomyces cerevisiae strain 211, was subjected to 250 kV X-rays or 50° C heat treatment for 30 min or to a combination of both. X-ray exposure took place either in air or in nitrogen. Cell number, percentage of budding cells and cell cycle progression was followed for up to 12 h post irradiation. The distribution of cell cycle stages was determined by flow cytofluorometry. All treatments cause a retardation of cell division rate. Hyperthermia leads mainly to a lengthening of G1, whereas X-rays arrest the cells reversibly in G2. The effect of the combined treatment appears to be merely additive. No selective action of hyperthermia on hypoxic cells was found.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01324370