Summary
Wild type diploid yeast, Saccharomyces cerevisiae strain 211, was subjected to 250 kV X-rays or 50° C heat treatment for 30 min or to a combination of both. X-ray exposure took place either in air or in nitrogen. Cell number, percentage of budding cells and cell cycle progression was followed for up to 12 h post irradiation. The distribution of cell cycle stages was determined by flow cytofluorometry. All treatments cause a retardation of cell division rate. Hyperthermia leads mainly to a lengthening of G1, whereas X-rays arrest the cells reversibly in G2. The effect of the combined treatment appears to be merely additive. No selective action of hyperthermia on hypoxic cells was found.
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Dedicated to Prof. Dr. A. Schraub on the occasion of his 70th birthday
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Fingerhut, R., Otto, F., Oldiges, H. et al. Cellular radiation effects and hyperthermia: Cytokinetic investigations with stationary phase yeast cells. Radiat Environ Biophys 18, 19–26 (1980). https://doi.org/10.1007/BF01324370
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DOI: https://doi.org/10.1007/BF01324370