ISSN:
1432-1211
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Expression of the Q5 k gene was examined by northern blot analysis and polymerase chain reaction (PCR) in the AKR mouse and various cell lines, each of the H-2 k haplotype. Our results show that Q5 k mRNA is present during the whole postimplantational development of the AKR embryo/fetus (gestation day 6 to 15). In the juvenile mouse (week 2 to 4) transcription of the Q5 k gene persisted in all organs examined. In contrast, in the adult animal expression of the Q5 k gene was limited to the thymus and uterus of the pregnant mouse. Upon malignant transformation, the amount of Q5 k -specific mRNA increased dramatically in thymus and could also be observed in the spleen of thymoma bearing animals. Expression of the Q5 k gene was also detectable in several transformed mouse cell lines. Mitogen stimulation or treatment with cytokines induced Q5 k expression in primary spleen cell cultures. A possible explanation for the tissue-restricted expression in the adult AKR mouse is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00212309
