Abstract
Expression of the Q5 k gene was examined by northern blot analysis and polymerase chain reaction (PCR) in the AKR mouse and various cell lines, each of the H-2 k haplotype. Our results show that Q5 k mRNA is present during the whole postimplantational development of the AKR embryo/fetus (gestation day 6 to 15). In the juvenile mouse (week 2 to 4) transcription of the Q5 k gene persisted in all organs examined. In contrast, in the adult animal expression of the Q5 k gene was limited to the thymus and uterus of the pregnant mouse. Upon malignant transformation, the amount of Q5 k-specific mRNA increased dramatically in thymus and could also be observed in the spleen of thymoma bearing animals. Expression of the Q5 k gene was also detectable in several transformed mouse cell lines. Mitogen stimulation or treatment with cytokines induced Q5 k expression in primary spleen cell cultures. A possible explanation for the tissue-restricted expression in the adult AKR mouse is discussed.
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Schwemmle, S., Bevec, D., Brem, G. et al. Developmental and tissue-specific expression of the Q5 k gene. Immunogenetics 34, 28–38 (1991). https://doi.org/10.1007/BF00212309
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DOI: https://doi.org/10.1007/BF00212309