ISSN:
1432-072X
Keywords:
Key words Catechol 2
;
3-dioxygenase
;
Pseudomonas putida
;
TOL plasmid
;
Monoclonal antibody
;
Immunoelectron microscopy
;
Quaternary structure
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Catechol 2,3-dioxygenase from the meta-cleavage pathway encoded on the TOL plasmid of Pseudomonas putida (pWWO) was investigated by electron microscopy. Negatively stained samples of the purified catechol 2,3-dioxygenase revealed that the enzyme consists of four subunits arranged in a tetrahedral conformation. Monoclonal antibodies raised against catechol 2,3-dioxygenase showed highly specific reactions and were used to localize the enzyme in Escherichia coli (pAW31) and P. putida (pWWO), using the protein A-gold technique carried out as a post-embedding immunoelectron microscopy procedure. Our in situ labeling studies revealed a cytoplasmic location of the catechol 2,3-dioxygenase in both cell types.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00262205
