Abstract
Catechol 2,3-dioxygenase from the meta-cleavage pathway encoded on the TOL plasmid of Pseudomonas putida (pWWO) was investigated by electron microscopy. Negatively stained samples of the purified catechol 2,3-dioxygenase revealed that the enzyme consists of four subunits arranged in a tetrahedral conformation. Monoclonal antibodies raised against catechol 2,3-dioxygenase showed highly specific reactions and were used to localize the enzyme in Escherichia coli (pAW31) and P. putida (pWWO), using the protein A-gold technique carried out as a post-embedding immunoelectron microscopy procedure. Our in situ labeling studies revealed a cytoplasmic location of the catechol 2,3-dioxygenase in both cell types.
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Abbreviations
- C23O :
-
Catechol 2,3-dioxygenase
- 3MB 3 :
-
Methylbenzoate
- AK1 :
-
Anti-C23O-IgG-antibody
- G :
-
Gold particle
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Winkler, J., Eltis, L.D., Dwyer, D.F. et al. Tetrameric structure and cellular location of catechol 2,3-dioxygenase. Arch. Microbiol. 163, 65–69 (1995). https://doi.org/10.1007/BF00262205
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DOI: https://doi.org/10.1007/BF00262205