ISSN:
1432-072X
Keywords:
Neurospora crassa
;
Morphological mutant
;
Cyclic adenosine 3′,5′-monophosphate
;
Protein kinase
;
Cyclic AMP-binding protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A cpk mutant of Neurospora crassa with morphological alteration was obtained spontaneously during the cross between the wild-type and a glycerol utilizing cr-l strain. The growth rate of cpk was intermediate between the wild-type and cr-1 mutant strains. The cpk conidia contained a reduced level of carotenoid pigments as compared to the wild-type conidia. The cpk mutant had no detectable amount of cyclic adenosine 3′,5′-monophosphate (cAMP)-binding protein at all stages of growth tested. On a DEAE-Sephacel column chromatogram, protein kinase activity of the wild type was eluted at two peaks; the first peak was cAMP-dependent, and the second one was not. In contrast, the cpk strain had two peaks of cAMP-independent enzymes. It is suggested that cAMP-dependent protein kinase may be altered in the cpk mutant into a cAMP-independent type by an alteration of the regulatory subunit of this enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00447052
